Hepatite crônica B oculta: prevalência e aspectos clínicos em população de elevada endemicidade de infecção pelo vírus da hepatite B na Amazônia Ocidental Brasileira

Júnior, Gildo Maia Barros; Braga, Wornei Silva Miranda; Oliveira, Cíntia Mara Costa de; Castilho, Márcia da Costa; Araújo, José de Ribamar

doi:10.1590/s0037-86822008000600010

Cited by 10 publications

(5 citation statements)

References 23 publications

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“…Wong et al reported 62% of OBI in a cohort of 48 HCC patients among them 80% of cryptogenic cases, 3% of HCV infection and 17% of alcohol related HCC [26]. On the other hand, Ferrari et al reported 4.4% of OBI in 62 paraffin-embedded liver tissue samples obtained from patients with diagnosis of liver cirrhosis [47]; Similarly, Barros-Junior et al reported 17.64% of OBI in 17 liver biopsies obtained from patients diagnosed with chronic hepatitis B [81]. …”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia

et al. 2017

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BackgroundHepatitis B virus (HBV) occult infection (OBI) is a risk factor to be taken into account in transfusion, hemodialysis and organ transplantation. The aim of this study was to identify and characterize at the molecular level OBI cases in patients with end-stage liver disease.MethodsSixty-six liver samples were obtained from patients with diagnosis of end-stage liver disease submitted to liver transplantation in Medellin (North West, Colombia). Samples obtained from patients who were negative for the surface antigen of HBV (n = 50) were tested for viral DNA detection by nested PCR for ORFs S, C, and X and confirmed by Southern-Blot. OBI cases were analyzed by sequencing the viral genome to determine the genotype and mutations; additionally, viral genome integration events were examined by the Alu-PCR technique.ResultsIn five cases out of 50 patients (10%) the criteria for OBI was confirmed. HBV genotype F (subgenotypes F1 and F3), genotype A and genotype D were characterized in liver samples. Three integration events in chromosomes 5q14.1, 16p13 and 20q12 affecting Receptor-type tyrosine-protein phosphatase T, Ras Protein Specific Guanine Nucleotide Releasing Factor 2, and the zinc finger 263 genes were identified in two OBI cases. Sequence analysis of the viral genome of the 5 OBI cases showed several punctual missense and nonsense mutations affecting ORFs S, P, Core and X.ConclusionsThis is the first characterization of OBI in patients with end-stage liver disease in Colombia. The OBI cases were identified in patients with HCV infection or cryptogenic cirrhosis. The integration events (5q14.1, 16p13 and 20q12) described in this study have not been previously reported. Further studies are required to validate the role of mutations and integration events in OBI pathogenesis.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia

et al. 2017

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“…Previously, Barros Jr et al (2008) assessed the occurrence of OBI in 51 patients with an anti-HBc-positive and HBsAg-negative profile in the Brazilian Amazon Region. Patients were tested by PCR, and 17% were found to exhibit this profile.…”

Section: Discussionmentioning

confidence: 99%

Detection and molecular characterisation of a diagnosis escape variant associated with occult hepatitis B virus in Brazil

Almeida

Mello

Menegoy

et al. 2017

Mem. Inst. Oswaldo Cruz

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BACKGROUND Many studies have identified mutations in the hepatitis B surface antigen (HBsAg) as important factors limiting the ability of commercial serological assays to detect this viral antigen. However, an association between mutations in the HBsAg gene and the occurrence of occult HBV infection (OBI) in patients has not been established.OBJECTIVES To detect hepatitis B virus (HBV) DNA in patients with anti-HBc as a unique serological marker, a previously published, cost-effective TaqMan-based real-time polymerase chain reaction (PCR) test with minor groove binding probes was adapted for use in this study. The current study also aimed to investigate HBsAg mutations and genotypes of HBV in OBI at the Viral Hepatitis Ambulatory Clinic in Rio de Janeiro to determine any possible association.METHODS Intra-assay and inter-assay reproducibility were determined, and the mean coefficient of variation values obtained were 2.07 and 3.5, respectively. Probit analysis indicated that the 95% detection level was 25 IU/mL. The prevalence of OBI was investigated in 35 serum samples with an ‘anti-HBc alone’ profile from individuals who attended our clinic between 2011 and 2013.FINDINGS HBV DNA was detected in only one sample, resulting in an OBI rate of 2.9%. Nucleotide sequencing of the pre-S/S region was performed to genotype and analyse mutations within the HBsAg gene of this HBV DNA. The HBV in the OBI case was classified as sub-genotype A1, and a sequence analysis of the small S gene revealed 12 mutations in the major hydrophilic region compared to the consensus A1 sequence. Most of these mutations occurred in amino acid residues that have been reported as clinically relevant because they have been implicated in vaccine escape and/or inability to detect HBsAg by commercial serological assays.MAIN CONCLUSIONS Our study suggests the importance of specific HBsAg mutations, different from those in D, B, and C genotypes, in sub-genotype A1 HBV associated with OBI.

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“…Por otra parte, siete artículos abordan el tema de la coinfección y la superinfección con el VHD. Ecuador, Brasil y Perú presentan mayor prevalencia de coinfección con 72%, 65% y 39%, respectivamente ( 11 , 17 , 18 ). Russell y cols.…”

Section: Resultsunclassified

Hepatitis B en indígenas de América Latina: una revisión de la literatura

Castro-Arroyave

Martínez-Gallego

Montoya-Guzmán

et al. 2022

Revista Panamericana De Salud Pública

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Objetivo. Identificar publicaciones realizadas entre los años 2000 y 2020 sobre hepatitis B en indígenas de América Latina, con el fin de conocer avances y vacíos en el tema durante los últimos 20 años. Métodos. Revisión exploratoria y síntesis rápida de la evidencia. El proceso de organización documental se realizó con los programas Excel® y Rayyan®. Resultados. Se seleccionaron 30 de 107 artículos encontrados, 17 corresponden a estudios epidemiológicos, 10 a revisiones documentales, dos estudios clínicos y una carta al editor. Brasil fue el país con más publicaciones (50%), la mayoría con enfoque epidemiológico. La temática más abordada fue la medición de prevalencia de la infección por hepatitis B con 22 publicaciones, le siguieron 11 estudios que informan resultados de estudios moleculares del virus, siete estudios sobre vacunación, cinco estudios sobre factores de riesgo y cuatro publicaciones con temas como la transmisión vertical y estudios sociales. Conclusión. En comparación con revisiones previas realizadas por otros autores, se observa una mayor diversidad en los temas y métodos de investigación utilizados; sin embargo, aún prevalecen los enfoques epidemiológicos convencionales centrados en la medición de la prevalencia de marcadores serológicos. Esto hace necesario encarar otro tipo de investigaciones centradas en los determinantes socioculturales.

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Hepatite crônica B oculta: prevalência e aspectos clínicos em população de elevada endemicidade de infecção pelo vírus da hepatite B na Amazônia Ocidental Brasileira

Cited by 10 publications

References 23 publications

Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia

Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia

Detection and molecular characterisation of a diagnosis escape variant associated with occult hepatitis B virus in Brazil

Hepatitis B en indígenas de América Latina: una revisión de la literatura

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