Hepatitis B is an infectious viral disease. The symptoms of this infection are vomiting sickness, tiredness, skin appears yellow in colour (yellowish skin), dark urine and pain in abdomen. The virus is mainly transmitted by blood or body fluids which are infected with viral pathogens. Health care workers and medical students in clinical years who come in contact with the patients are at the highest risk of acquiring this kind of infection. This study deals with the knowledge, attitude and awareness of hepatitis B vaccine. Questionnaire survey was conducted among 100 medical college students by giving them 20 questions based on the awareness of hepatitis B infection, mode of its transmission, dues of vaccination, and any booster dose. The obtained results were analysed in SPSS software. Descriptive and association statistics were done with chi square test. Based on the result it is shown that 64 % of students were aware of hepatitis B. 61% of students were aware about the cause of Hepatitis B. 73 % of people were aware about the Ideal age for vaccination. 41 % of students are aware about the Hepatitis B through doctors awareness programs and 38% of students are aware through the media. From the survey done it is proven that the knowledge, attitude and awareness of hepatitis B vaccine among medical and dental students was prevalent but not followed by the students due to their negligence.