Hepatitis B Virus Genetic Typing Using Mass-Spectrometry

Malakhova, M V; Ilina, Elena N.; Govorun, Vadim M.; Shut'ko, S A; Dudina, Kristina; Znoyko, O. O.; Климова, Е А; Iushchuk, N D

doi:10.1007/s10517-009-0479-1

Cited by 10 publications

(4 citation statements)

References 14 publications

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“…Viruses present a particular challenge to typing assays because their replication machinery generates a high error rate, resulting in the extensive genetic variability of the intra-and interhost viral populations. One of the first HBV genotyping assays adapted to MS was based on MALDI-TOF [63]. Recently, an improved approach to HBV genotyping was designed and implemented using the MassARRAY platform.…”

Section: Pathogen Genotypingmentioning

confidence: 99%

Application of mass spectrometry to molecular diagnostics of viral infections

Ganova-Raeva¹,

Khudyakov

2013

Expert Review of Molecular Diagnostics

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Mass spectrometry (MS) has found numerous applications in life sciences. It has high accuracy, sensitivity and wide dynamic range in addition to medium-to high-throughput capabilities. These features make MS a superior platform for analysis of various biomolecules including proteins, lipids, nucleic acids and carbohydrates. Until recently, MS was applied for protein detection and characterization. During the last decade, however, MS has successfully been used for molecular diagnostics of microbial and viral infections with the most notable applications being identification of pathogens, genomic sequencing, mutation detection, DNA methylation analysis, tracking of transmissions, and characterization of genetic heterogeneity. These new developments vastly expand the MS application from experimental research to public health and clinical fields. Matching of molecular techniques with specific requirements of the major MS platforms has produced powerful technologies for molecular diagnostics, which will further benefit from coupling with computational tools for extracting clinical information from MS-derived data.

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Section: Pathogen Genotypingmentioning

confidence: 99%

Application of mass spectrometry to molecular diagnostics of viral infections

Ganova-Raeva¹,

Khudyakov

2013

Expert Review of Molecular Diagnostics

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“…Polymerase chain reaction-mass spectrometry (PCR-MS) has been used to detect/identify infectious pathogens (Fox, 2006;Sampath et al, 2007a;Eshoo et al, 2010;Fabris, 2010). The detection of SNPs with a MALDI-MS-based minisequencing method has been used to identify hepatitis B virus in HBsAgpositive patients with chronic hepatitis B (Malakhova et al, 2009) and to detect drug resistance-related mutations in N. gonorrhoeae (Vereshchagin et al, 2005) and M. tuberculosis (Ikryannikova et al, 2007). The same method has been successfully applied to the rapid detection of clinically significant TEM-type extended-spectrum beta-lactamases in clinical strains of E. coli and Klebsiella pneumonia (Ikryannikova et al, 2008).…”

Section: Applicationsmentioning

confidence: 99%

Advances in mass spectrometry for the identification of pathogens

Reddy

2011

Mass Spectrometry Reviews

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Mass spectrometry (MS) has become an important technique to identify microbial biomarkers. The rapid and accurate MS identification of microorganisms without any extensive pretreatment of samples is now possible. This review summarizes MS methods that are currently utilized in microbial analyses. Affinity methods are effective to clean, enrich, and investigate microorganisms from complex matrices. Functionalized magnetic nanoparticles might concentrate traces of target microorganisms from sample solutions. Therefore, nanoparticle-based techniques have a favorable detection limit. MS coupled with various chromatographic techniques, such as liquid chromatography and capillary electrophoresis, reduces the complexity of microbial biomarkers and yields reliable results. The direct analysis of whole pathogenic microbial cells with matrix-assisted laser desorption/ionization MS without sample separation reveals specific biomarkers for taxonomy, and has the advantages of simplicity, rapidity, and high-throughput measurements. The MS detection of polymerase chain reaction (PCR)-amplified microbial nucleic acids provides an alternative to biomarker analysis. This review will conclude with some current applications of MS in the identification of pathogens.

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“…Given the vital role of genomic progress in the HBV life cycle, conventional detection technologies-including polymerase chain reaction (PCR), uorescence in situ hybridization (FISH), electrochemical sensing and mass spectrometry-have been conducted in this research eld. [13][14][15][16] Although these methods exhibit favorable detection performance with high sensitivity and selectivity, the shortcomings of labor-intensive, timeconsuming, enzyme-demanding, and complicated chemical modication create a noticeable burden inuencing their efficiency and universality in real detection scenarios. To address these challenges, several facile colorimetric or uorescent detection methods based on nanomaterials have been developed for accurate and point-of-care analysis of the HBV DNA genome in real samples.…”

Section: Introductionmentioning

confidence: 99%