Hepatitis B virus preS1-derived lipopeptide functionalized liposomes for targeting of hepatic cells

Carbohydrate‐Based Nanocarriers Exhibiting Specific Cell Targeting with Minimum Influence from the Protein Corona

“…Them annose-functionalized HES-NCs exhibit binding to the enzyme,h owever,t he protein corona influenced the binding of C-type lectin:w ith adsorbed plasma proteins,t he binding of C-type lectin is reduced but clear binding can still be detected (Figure 3). These results demonstrate that PEGylated nanocarriers can be further functionalized with targeting groups,f or example,m annose,i no rder to specifically reach both immature and mature dendritic cells,even in the presence of plasma proteins.T he interaction between plasma proteins and surface-bound mannose does not prevent it from being recognized by specific acceptors.T his finding is in accordance with other works demonstrating minor protein adsorption to polysaccharides including HES, [25] chitosan, [26] hyaluronic acid. However, as phagocytis cells,i DCs are known to also take up NCs unspecifically.I no rder to verify that after adsorption of plasma proteins,the uptake of mannose-functionalized HES-NCs into DCs is ar eal receptor-mediated phenomenon, mature dendritic cells (mDCs) were also investigated.…”

“…Figure 4s hows as imilar uptake of HES-PEG5000-C1-Man into mDCs before ( Figure 4A)a nd after ( Figure 4B) incubation with human plasma ( Figure S5 shows the corresponding microscopy images). These results demonstrate that PEGylated nanocarriers can be further functionalized with targeting groups,f or example,m annose,i no rder to specifically reach both immature and mature dendritic cells,even in the presence of plasma proteins.T he interaction between plasma proteins and surface-bound mannose does not prevent it from being recognized by specific acceptors.T his finding is in accordance with other works demonstrating minor protein adsorption to polysaccharides including HES, [25] chitosan, [26] hyaluronic acid. [27] In conclusion, hydroxyethyl starch nanocarriers were efficiently PEGylated and functionalized at the outer-layer with amino derivatives of mannose by aconvenient two-step method that relies on sequential nucleophilic additions to isocyanates.T he influence of mannose attached to the PEGylated NCs on the pattern of the protein corona after plasma incubation was shown to be minimal by ITC,D LS, SDS-PAGE, and mass spectrometry.F urthermore,c ellular uptake of the NCs by dendritic cells and abinding assay with C-type lectin showed that the targeting moieties are accessible to the biological receptors after incubation with plasma.…”

Carbohydrate nanocarriers in biomedical applications: functionalization and construction

“…(i) protecting the payload 133 and increasing the plasma halflife times; 23,24,137 (ii) specific targeting; 132,136,137,167 (iii) enzyme-induced release or activation of a therapeutic agent; [23][24][25][26][27] (v) decreased toxicity of the payload; 131,134,136,167 (vi) kinetics of metabolization are tunable by chemical functionalization to balance biocompatibility and stability. 23,24 As carbohydrates are omnipresent as functional surface coatings in nature, their use in biomedical applications is obvious.…”

Tumor-Targeted Paclitaxel Delivery and Enhanced Penetration Using TAT-Decorated Liposomes Comprising Redox-Responsive Poly(Ethylene Glycol)

“…Furthermore, no significant inhibition of cellular uptake was observed in presence of nocodazole (microtubule‐active agent), which suggested that microtubule function was not necessary. Additionally, the uptake of C‐TAT‐LP (in the presence of GSH) was strongly decreased by 39.5% ( p < 0.01) and 28.8% ( p < 0.05), respectively, in the presence of colchicine (macropinocytosis‐mediated endocytosis pathway inhibitor) or Filipin (caveolae‐mediated endocytosis pathway inhibitor), but chlorpromazine (clathrin‐mediated endocytosis pathway inhibitor) exerted little inhibition efficacy on B16F1 cells. This illustrated the active endocytosis of C‐TAT‐LP (in the presence of GSH) was mediated by macropinocytosis with the involvement of cell skeleton and lysosome.…”