Background: to determine the prevalence of HBs Ag positive in staff of Amir Kabir Hospital (referral center) and compare with the results given by others.Methods: 202 cases, working in Amir Kabir Hospital for more than 5 years were selected. ELISA Micro Plate Kit (Radim Company Italy) and Heponostrik kit were used for estimation. Positive samples were re-tested by the same method and confirm them by Iranian Blood Transfusion Organization, Arak branch.Results: Our results showed that prevalence of infection to hepatitis B among Amir Kabir Hospital staff 2.9 %.The ratio of male to female were 2.4. However out of positive cases 83.4 % were male and 16.6 % female .All the positive cases had the previous contact with the blood or discharges of patients and needle.
Conclusion:The results of present study showed increment in infection of male staff to HBsAg as compared to the female .The rate of positive HBs Ag was 2.9 %, which showed increment as compared the results of 0.6-1.6% given by other