Hepatitis C Virus Genotyping in Saudi Population

Abstract

Background: Hepatitis C is one of the common types of viral hepatitis that threatens human life worldwide. Hepatitis C virus (HCV) is a single-strand RNA virus that has the ability to be transmitted by contaminated blood products and intravenous drug use. HCV is characterized by its ability to have a high genetic diversity, which eventually results in different HCV genotypes and numerous subtypes; however, this genetic variety is a core reason for the lack of a successful vaccine for these types of viruses. Methods: HCV nucleic acid was isolated from 597 patients diagnosed with chronic hepatitis C in Security Forces Hospital (SFHR) in Riyadh. HCV nucleic acid was extracted to make the target accessible for amplification and genotype detection. The process of nucleic acid extraction and amplification was accomplished with the Abbott Molecular Systems m2000sp and m2000rt using the Abbott RealTime HCV Genotype II. Results: The findings in this study illustrated a major prevalence of genotype 4 (60% of cases). The study also showed a large number of patients (27%) identified as HCV genotype 1. Furthermore, the HCV genotype 1 testing detected subtypes 1a and 1b in a number of patients. It was also demonstrated that a limited number of hepatitis C patients had mixed genotypes and undermined genotypes. Conclusion: The study has confirmed that HCV genotype 4 has a high prevalence when compared to the rest of the identified genotypes. Furthermore, the majority of identified genotypes and subtypes were found to be linked with age more than 50 years, which might indicate an association of HCV genotype detection with age.

Distribution of hepatitis C virus (HCV) genotypes in a Saudi Arabian hospital during the 2015-2020 period

“…Variations in genotype prevalence present challenges to the development of vaccines and therapeutics. The geographic distribution of HCV genotypes varies, and the transmission mode may influence the dominance of specific genotypes [3,12]. The study demonstrated the difference in the distribution of hepatitis C virus genotypes between the age groups of patients 50 years and older and less than 50 years.…”

“…In this study, most chronically HCV-infected individuals were infected with GT-2 and GT-4 (33.1% and 25.8%), respectively. In Saudi Arabia, GT-4 was cited as the most common HCV genotype [2,9,12]. In addition, few studies reported a high prevalence of GT-4 across Saudi Arabia [2,9,12].…”

“…In Saudi Arabia, GT-4 was cited as the most common HCV genotype [2,9,12]. In addition, few studies reported a high prevalence of GT-4 across Saudi Arabia [2,9,12].…”

Prevalence of Hepatitis C Viral Infection among Diabetes Mellitus Patients in Qassim Region, Saudi Arabia

“…18 Genotypes 1 and 4 are responsible for 86% of chronic HCV infections in Saudi Arabia. [19][20][21] HCV is primarily transmitted through exposure of infected blood. 22 Unsafely performed injection practices is the most predominant mode for transmission and it accounts to 40% of HCV infections reported worldwide.…”