Hepatitis E Virus Replication Requires an Active Ubiquitin-Proteasome System

Karpe, Yogesh A.; Meng, Xiang‐Jin

doi:10.1128/jvi.07039-11

Cited by 45 publications

(39 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accumulating evidence has shown that UPS is involved in numerous cellular activities, such as cell cycle control, apoptosis, and immune responses (Choudhury et al, 2013;Rastogi and Mishra, 2012;Tu et al, 2012). It has been demonstrated that UPS is important for the replication of positive-stranded RNA viruses such as CVB3, hepatitis E virus, and dengue virus (Kanlaya et al, 2010;Karpe and Meng, 2012;Si et al, 2007). In the present study, we demonstrated that EV71 infection promoted ubiquitination, while the activity of proteasome remained unchanged.…”

Section: Discussioncontrasting

confidence: 36%

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

Lin

Qin

et al. 2015

Virus Research

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 36%

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

Lin

Qin

et al. 2015

Virus Research

View full text Add to dashboard Cite

“…HEV may also manipulate the UPS to regulate its life cycle, especially since inhibiting an active UPS affects HEV replication, possibly by inhibiting transcription or translation (31). Overexpression of ubiquitin in inhibitortreated cells partially reverses the inhibitor effect on HEV replication (31). Although the influence of PPR ubiquitination on the regulation of virus replication must still be demonstrated, these findings suggest that the UPS regulates HEV replication.…”

Section: Discussionmentioning

confidence: 90%

“…Ubiquitination of the polymerase of coxsackievirus B3 is also necessary for completion of the virus life cycle (30). HEV may also manipulate the UPS to regulate its life cycle, especially since inhibiting an active UPS affects HEV replication, possibly by inhibiting transcription or translation (31). Overexpression of ubiquitin in inhibitortreated cells partially reverses the inhibitor effect on HEV replication (31).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Polyproline Region of the Hepatitis E Virus in Immunocompromised Patients

Lhomme

Abravanel

Dubois

et al. 2014

J Virol

View full text Add to dashboard Cite

Little is known about virus adaptation in immunocompromised patients with chronic genotype 3 hepatitis E virus (HEV3) infections. Virus-host recombinant strains have been isolated recently from chronically infected patients. The nature and incidence of such recombinant events occurring during infections of solid-organ transplant (SOT) recipients are essentially unknown. The polyproline region (PPR) of strains isolated from SOT patients was sequenced during the acute-infection phase (n ‫؍‬ 59) and during follow-up of patients whose infections became chronic (n ‫؍‬ 27). These 27 HEV strains included 3 (11%) that showed recombinant events 12, 34, 48, or 88 months after infection. In one strain, parts of the PPR and the RNA-dependent RNA polymerase were concomitantly inserted. In the second, a fragment of a human tyrosine aminotransferase (TAT) gene was inserted first, followed by a fragment of PPR. A fragment of the human inter-␣-trypsin inhibitor (ITI) gene was inserted in the third. All the inserted sequences were rich in aliphatic and basic amino acids. In vitro growth experiments suggest that the ITI insertion promoted more vigorous virus growth. In silico studies showed that the inserted sequences could provide potential acetylation, ubiquitination, and phosphorylation sites. We found that recombinant events had occurred in the HEV PPR in approximately 11% of the strains isolated from chronically infected transplant patients followed up in Toulouse University Hospital. These inserted fragments came from the HEV genome or a human gene and could enhance virus replication. IMPORTANCE Hepatitis E virus (HEV) can cause chronic infections in immunocompromised patients, including solid-organ transplant (SOT)recipients. Two strains that had undergone recombination with human ribosomal genes were described recently. The strains with inserted sequences replicated better in vitro. Little is known about the frequency of such recombinant events or how such an insertion enhances replication. We therefore investigated 59 SOT patients infected with HEV and found 3 strains with 4 recombinant events in 27 of these patients whose infection became chronic. The 4 inserted sequences were of different origins (human gene or HEV genome), but all were enriched in aliphatic and basic amino acids and provided potential regulation sites. Our data indicate that recombinant events occur in approximately 11% of strains isolated from chronically infected patients. The structures of the inserted sequences provide new clues as to how the inserted sequences could foster virus replication.

show abstract

“…Recently Ramakrishnaiah et al (2013) suggested that HCV might transmit through exosomes to evade the immune response. It has been also demonstrated that an active ubiquitin-proteasome system plays an important role in Hepatitis E virus replication (Karpe and Meng, 2012). Identification of DIS3, PTK-7 and CD3 as UTR interacting proteins suggest that exosomes might play important roles in the immune response against HEV.…”

Section: Discussionmentioning

confidence: 97%

Identification and characterization of cellular proteins interacting with Hepatitis E virus untranslated regions

Paingankar

Arankalle

2015

Virus Research

View full text Add to dashboard Cite

Hepatitis E Virus Replication Requires an Active Ubiquitin-Proteasome System

Cited by 45 publications

References 52 publications

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

Characterization of the Polyproline Region of the Hepatitis E Virus in Immunocompromised Patients

Identification and characterization of cellular proteins interacting with Hepatitis E virus untranslated regions

Contact Info

Product

Resources

About