Hepatitis G viral RNA in Italian haemophiliacs with and without hepatitis C infection

Tagariello, Giuseppe; Infantolino, D.; Biasin, Maria Raffaella; Davoli, Pier Giorgio; Traldi, A

doi:10.1016/s0140-6736(05)65677-2

Cited by 18 publications

(13 citation statements)

References 4 publications

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“…High prevalence rates for both GBV-C and HCV were observed in the group of hemophiliacs studied and in all age groups in agreement with previous studies reporting a high risk for HCV infection [Brettler et al, 1990;Rumi et al, 1990; Makris et al, 1990; Simmonds Naoumov and Rumi, 1991] as well as an increased risk for GBV-C infection in patients with this background [Schleicher et al, 1996;Tagariello et al, 1996]. Dual infections have also been reported owing to both viruses sharing transmission pathways [Masuko et al, 1996;Nü bling and Lower, 1996;Feucht et al, 1996;Linnen et al, 1996].…”

Section: Discussionsupporting

confidence: 91%

High prevalence of GB virus C strains genetically related to strains with Asian origin in Nicaraguan hemophiliacs

et al. 1997

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The presence of hepatitis GB virus C (GBV-C), also known as hepatitis G virus (HGV), and hepatitis C virus (HCV) were investigated in sera from 45 hemophiliacs from nine locations in Nicaragua using a nested polymerase chain reaction (PCR). Primers used to detect GBV-C and HCV derived from the helicase region and 5'UTR, respectively. Seventeen (38%) patients were positive for GBV-C RNA in serum by PCR. Twelve (27%) patients were positive for HCV RNA by PCR. Six (13%) of these were coinfected with GBV-C. Anti-HCV was detected in all the 12 HCV RNA positive hemophiliacs and in another 14 (31%) individuals, in whom GBV-C RNA was found in 2. Ten patients (22%) lacked markers for both GBV-C and HCV. The mean age of the patients positive for GBV-C but negative for HCV by PCR was significantly lower than for those negative for GBV-C but positive for HCV by PCR (P < 0.05; Student's t-test), indicating that the risk for this group of hemophiliacs to acquire GBV-C infection is higher as compared to the risk of acquiring HCV infection. Eleven GBV-C strains were sequenced in the 5'UTR. Sequence comparison to previously published GBV-C strains revealed that all 11 strains were more similar to Asian strains than to strains of European and African origin. Sequences in the NS5-B region were available for 8 HCV strains, all of which were found to belong to genotype 1a. The similarity of the Nicaraguan GBV-C strains to strains from Asia indicates that the GBV-C strains in the region presumably have an Amerindian origin. It is also considered that the HTLV II strains in the New World aboriginal populations are ancient and brought there by the ancestral Amerindian populations from Asia. Further, the genotype F of hepatitis B virus, known to represent the strains in populations with Amerindian background, predominates in Central American populations with Hispanic background. It remains to be clarified why Amerindian strains of GBV-C as well as of HBV predominate also in populations with mixed ethnic background in Central America.

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Section: Discussionsupporting

confidence: 91%

High prevalence of GB virus C strains genetically related to strains with Asian origin in Nicaraguan hemophiliacs

et al. 1997

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“…This compares with a rate of 18% in U.S.A. haemophilic patients reported by Linnen et al (1996) and a rate of 6% in 70 Italian patients with severe haemophilia, a number of whom were young patients mainly treated with virally inactivated concentrates (Tagariello et al, 1996).…”

Section: Discussionsupporting

confidence: 49%

Hepatitis G virus infection in patients with bleeding disorders

et al. 1997

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Eighty‐two patients with bleeding disorders registered with our centre were screened for infection with hepatitis G virus (HGV). 80 patients were positive for hepatitis C (HCV) antibodies, 66 of whom (83%) were HCV PCR positive. 11 patients (13%) were HGV RNA‐positive, a similar prevalence rate to that of other studies of patients with bleeding disorders who received factor concentrates prior to the introduction of viral inactivation procedures. There was no significant difference in histological activity index (HAI) between the 10 HGV RNA‐positive and the 31 HGV RNA‐negative patients who underwent liver biopsy for assessment of HCV infection (median HAI scores 5.5, range 2–10 and four, range 0–10 respectively, P = 0.07). One patient in each group had established cirrhosis. In patients who underwent HCV quantitation there was no significant difference in HCV viral titre between HGV RNA‐positive and negative patients (median HCV titre in HGV RNA‐positive patients 2.10 × 105 DNA copies /ml (n = 8) range 4.17 × 102 to 4.17 × 106, median HCV titre in HGV RNA‐negative patients 3.33 × 105 (n = 31) range 1.00 × 103 to 6.67 × 106, P = 0.68). In this study there was no evidence that individuals co‐infected with HGV and HCV have more severe liver disease than those infected with HCV alone.

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“…Jarvis et al (1996) reported high frequencies of contamination with HGV RNA in batches of archived coagulation factor concentrates prepared without viral inactivation procedures. A high prevalence of HGV RNA has been reported in Italian haemophiliacs (Tagariello et al, 1996) and in Belgian haemophilacs (Sheng et al, 1996), but the pathological significance of hepatitis G virus infection remains unclear. In this study we investigated the presence of HGV/GBV-C RNA in sera from 92 haemophiliacs.…”

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confidence: 99%

Prevalence of hepatitis G virus RNA in a monocentric population of French haemophiliacs

Gérolami

Halfon

Chambost³

et al. 1997

Br J Haematol

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Summary. Hepatitis G virus (HGV) and hepatitis GB virus (GBV-C) have been reported as possible causes of non-A-E transfusional hepatitis. To assess the prevalence of hepatitis G virus infection in haemophiliacs we retrospectively investigated the presence of viral RNA in 92 patients with and without HCV infection. HGV/GBV-C RNA was reverse transcribed and amplified with primers from the 5 0 noncoding region of the genome. RNA was detected in 16/92 patients (17 . 4%). Restriction enzyme analysis revealed that the 16 patients belonged to the HGV-like genotype. Serology with E2-specific antibodies demonstrated that HGV viraemia underestimates previous infection by HGV. 33 patients were positive for HGV; all but two have cleared HGV RNA. 47/92 patients had a marker of prior infection by HGV.No difference between HGV RNA positive and negative patients was observed concerning age, diagnosis, HIV and HCV status. Previous HBV infection correlated with the frequency of HGV infection. There was no difference in alanine aminotransferase levels between HGV positive and negative patients. All 18 patients exposed to only virally inactivated plasma-derived concentrates were negative for both HGV RNA and anti E2 antibodies.Prior exposure to untreated concentrates correlated with HGV viraemia (P¼0 . 03), HGV seropositivity (P¼0 . 0002), and markers of HGV infection (P<0 . 0001).In haemophiliacs with a past exposure to non-inactivated concentrates, persistence of HCV RNA (53/74 patients) was more frequent than HGV RNA persistence (16/74 patients) although HGV viraemia is more frequent than HCV viraemia in blood donors. This may be related to a greater ability of individuals to clear HGV infection and suggests that hepatitis G virus infection in multi-transfused patients has a better outcome than infection with other blood-borne viruses.

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Hepatitis G viral RNA in Italian haemophiliacs with and without hepatitis C infection

Cited by 18 publications

References 4 publications

High prevalence of GB virus C strains genetically related to strains with Asian origin in Nicaraguan hemophiliacs

High prevalence of GB virus C strains genetically related to strains with Asian origin in Nicaraguan hemophiliacs

Hepatitis G virus infection in patients with bleeding disorders

Prevalence of hepatitis G virus RNA in a monocentric population of French haemophiliacs

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