Hepatocellular and Immuno-toxicopathological Effects of Acrylonitrile in Albino Male Mice

Abstract

This study to provide details on role of Acrylonitrile (AN) in blood profile and histopathological changes in liver in male albino mice, the aims of current study were detected influence and toxication of AN in the male mice, the present article was include (30 albino mice) divided into 3 groups, the age ranged from 8-9 week. The dose of Acrylonitrile (25mg / kg of BW) and the period of this study 60 days.The hematological examination showed increased in WBCs (9.32±1.11 / 11.802±0.75), Lymphocyte (9.25±2.20 / 11.05±1.95), Neutrophile (5.55±0.88 / 7.03±1.50) and Monocyte (0.19±0.97 / 0.33±0.18) in 2 nd and 3 rd group respectively, while showed decreased in RBCs (6.40±1.11 / 5.82±0.70), PCV (38.65±1.20 / 36.40±2.13), MCV (44.70±1.49 /41.95±0.49), MCH (14.85±1.44 / 10.00±1.84) and Hb (13.24±1.82 / 12.63±1.91) in 2 nd and 3 rd group respectively, The histopathological changes in liver showed extensive necrosis, thickening and congestion of blood vessels, infiltration of inflammatory cells, mast cell aggregation in hepatocytes, hepatocyte atrophy and extensive areas of abscess in 2 nd group, while in 3 rd group showed perihyperplastic bile duct, large extensive granuloma, multiple giant cells, dystrophic calcified and hepatic necrosis.