Hepatocellular carcinoma-associated antigen 59 and ADP-ribosylation factor 1 with poly (lactic-co-glycolic acid): A promising candidate as nanovaccine against haemonchosis

Wang, Qiangqiang; Muhammad, Tahir Aleem; Muhammad, Waqqas Hasan; Muhammad, Ali; Haseeb, Muhammad; Ren, Yan; Xu, Lixin; Song, Xiaoling; Hasan, Muhammad Waqqas

doi:10.1016/j.micpath.2022.105614

Cited by 3 publications

(2 citation statements)

References 62 publications

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“…Suppressor of cytokine signaling (SOCS) proteins were identified as the inducible feedback inhibitors of various hematopoietic cytokines triggering the JAK/STAT signaling pathway, playing an important role in inhibiting immunity and inflammation [40,41]. ADPribosylation factor 1 (ARF1) is recognized to largely regulate the immune responses of host cells in Haemonchus contortus [42]. The transporter ADP ribosylation factor 1(ARF1) differentially regulated humoral immunity in Drosophila and played a pivotal part in regulating the cellular immune response by controlling the crystal cell melanization and phenoloxidase activity with the blood cell-specific endosomal regulator ASRIJ [43].…”

Section: Discussionmentioning

confidence: 99%

First Characterization and Regulatory Function of piRNAs in the Apis mellifera Larval Response to Ascosphaera apis Invasion

Sun,

Fan,

Long

et al. 2023

IJMS

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piRNAs are a class of small non-coding RNAs that play essential roles in modulating gene expression and abundant biological processes. To decode the piRNA-regulated larval response of western honeybees (Apis mellifera) to Ascosphaera apis infection, the expression pattern of piRNAs in Apis mellifera ligustica larval guts after A. apis inoculation was analyzed based on previously obtained high-quality small RNA-seq datasets, followed by structural characterization, target prediction, regulatory network investigation, and functional dissection. Here, 504, 657, and 587 piRNAs were respectively identified in the 4-, 5-, and 6-day-old larval guts after inoculation with A. apis, with 411 ones shared. These piRNAs shared a similar length distribution and first base bias with mammal piRNAs. Additionally, 96, 103, and 143 DEpiRNAs were detected in the 4-, 5-, and 6-day-old comparison groups. Targets of the DEpiRNAs were engaged in diverse pathways such as the phosphatidylinositol signaling system, inositol phosphate metabolism, and Wnt signaling pathway. These targets were involved in three energy metabolism-related pathways, eight development-associated signaling pathways, and seven immune-relevant pathways such as the Jak-STAT signaling pathway. The expression trends of five randomly selected DEpiRNAs were verified using a combination of RT-PCR and RT-qPCR. The effective overexpression and knockdown of piR-ame-945760 in A. apis-infected larval guts were achieved by feeding a specific mimic and inhibitor. Furthermore, piR-ame-945760 negatively regulated the expression of two target immune mRNAs, SOCS5 and ARF1, in the larval gut during the A. apis infection. These findings indicated that the overall expression level of piRNAs was increased and the expression pattern of piRNAs in larval guts was altered due to the A. apis infection, DEpiRNAs were putative regulators in the A. apis-response of A. m. ligustica worker larvae. Our data provide not only a platform for the functional investigation of piRNAs in honeybees, especially in bee larvae, but also a foundation for illuminating the piRNA-involved mechanisms underlying the host response to the A. apis infection.

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Section: Discussionmentioning

confidence: 99%

First Characterization and Regulatory Function of piRNAs in the Apis mellifera Larval Response to Ascosphaera apis Invasion

Sun,

Fan,

Long

et al. 2023

IJMS

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“…However, these molecular mechanisms are yet to be explored and need further in-depth research. Therefore, to control parasitic infections researchers are actively exploring alternative methods such as nanovaccine ( 14 , 15 ), nanoparticle ( 16 ), and plant extracts have shown promising anti-parasitic activity to counter anthelminthic resistance in H. contortus ( 17–19 ).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants

Ahmad,

Khan,

Majid

et al. 2024

Front. Vet. Sci.

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IntroductionHaemonchus contortus (H. contortus) is a blood-feeding nematode causing infectious disease haemonchosis in small ruminants of tropical and subtropical regions around the world. This study aimed to explore the prevalence and phylogeny of H. contortus in small ruminants using the internal transcribed spacer-2 (ITS-2) gene. In addition, a comprehensive review of the available literature on the status of H. contortus in Pakistan was conducted.MethodsFecal samples were collected from sheep and goats (n = 180). Microscopically positive samples were subjected to DNA extraction followed by PCR using species-specific primers.ResultsThe overall prevalence of H. contortus was 25.55% in small ruminants. The prevalence of H. contortus was significantly associated with months and area. The highest occurrence of haemonchosis was documented in July (38.70%), whereas the lowest occurred in December (11.11%), with significant difference. The prevalence was highest in the Ghamkol camp (29.4%) and lowest in the arid zone of the Small Ruminant Research Institute (17.5%) (p = 0.01). The results of the systematic review revealed the highest prevalence of haemonchosis (34.4%) in Khyber Pakhtunkhwa (p = 0.001).DiscussionPhylogenetic analysis revealed a close relationship between H. contortus and isolates from Asia (China, India, Iran, Bangladesh, Malaysia, and Mongolia) and European countries (Italy and the United Kingdom). It has been concluded that H. contortus is prevalent in small ruminants of Kohat district and all over Pakistan, which could be a potential threat to food-producing animals, farmers, dairy, and the meat industry. Phylogenetic analysis indicates that H. contortus isolates share close phylogenetic relationships with species from Asia and Europe.

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Encapsulation of Adp-Ribosylation Factor 1 within Polymer (Plga) Mitigates the Fecal Egg Burden in Goats Challenged with Haemonchus Contortus

Hasan,

Gadahi,

Ehsan

et al. 2024

Preprint

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Hepatocellular carcinoma-associated antigen 59 and ADP-ribosylation factor 1 with poly (lactic-co-glycolic acid): A promising candidate as nanovaccine against haemonchosis

Cited by 3 publications

References 62 publications

First Characterization and Regulatory Function of piRNAs in the Apis mellifera Larval Response to Ascosphaera apis Invasion

First Characterization and Regulatory Function of piRNAs in the Apis mellifera Larval Response to Ascosphaera apis Invasion

Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants

Encapsulation of Adp-Ribosylation Factor 1 within Polymer (Plga) Mitigates the Fecal Egg Burden in Goats Challenged with Haemonchus Contortus

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