Hepatocellular Carcinoma Is the Most Frequent Final Diagnosis of Focal Liver Lesions Identified in a Cross-Sectional Evaluation of Patients with Chronic Liver Disease in Saudi Arabia

Agha, Adnan; Furnari, Manuele; Chakik, Rafaat; Ali, Mamdouh M.; Alsaudi, Dib; Bazeed, Mohammed; Savarino, Vincenzo; Giannini, Edoardo G.

doi:10.1155/2015/492782

Cited by 1 publication

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cancer rates are rising worldwide, and breast cancer is considered to be the second leading cause tumor-related deaths in women in Saudi Arabia 48 , with colon cancer being the third leading cause of death, and a high mortality rate observed for breast cancer worldwide 49 . Liver malignancy is another common diagnosis in middle-eastern patients who suffer from chronic liver disease 50 . The toxicity of the ASNase produced by B. licheniformis in this study was evaluated for cancer cell lines, the results of which showed that the enzyme was highly toxic toward HepG-2 cells, more so than against MCF-7 and HCT-116 cells.…”

Section: Discussionmentioning

confidence: 99%

Production and Anticancer Activity of an L-Asparaginase from Bacillus licheniformis Isolated from the Red Sea, Saudi Arabia

Alrumman

Mostafa

Al-izran

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Microbial L-asparaginase (ASNase) is an important anticancer agent that is used extensively worldwide. In this study, 40 bacterial isolates were obtained from the Red Sea of Saudi Arabia and screened for ASNase production using a qualitative rapid plate assay, 28 of which were producing large L-asparagine hydrolysis zones. The ASNase production of the immobilized bacterial cells was more favorable than that of freely suspended cells. A promising isolate, KKU-KH14, was identified by 16S rRNA gene sequencing as Bacillus licheniformis . Maximal ASNase production was achieved using an incubation period of 72 h, with an optimum of pH 6.5, an incubation temperature of 37 °C, an agitation rate 250 rpm, and with glucose and (NH 4 ) 2 SO 4 used as the carbon and nitrogen sources, respectively. The glutaminase activity was not detected in the ASNase preparations. The purified ASNase showed a final specific activity of 36.08 U/mg, and the molecular weight was found to be 37 kDa by SDS-PAGE analysis. The maximum activity and stability of the purified enzyme occurred at pH values of 7.5 and 8.5, respectively, with maximum activity at 37 °C and complete thermal stability at 70 °C for 1 h. The K m and V max values of the purified enzyme were 0.049995 M and of 45.45 μmol/ml/min, respectively. The anticancer activity of the purified ASNase showed significant toxic activity toward HepG-2 cells (IC 50 11.66 µg/mL), which was greater than that observed against MCF-7 (IC 50 14.55 µg/mL) and HCT-116 cells (IC 50 17.02 µg/mL). The results demonstrated that the Red Sea is a promising biological reservoir, as shown by the isolation of B. licheniformis , which produces a glutaminase free ASNase and may be a potential candidate for further pharmaceutical use as an anticancer drug.

show abstract

Section: Discussionmentioning

confidence: 99%