Hepatocellular transplantation into the lung for temporary support of acute liver failure in the rat

Sandbichler, P.; P, Then; Vogel, Wolfgang; Erhart, Reinhold; Dietze, Otto; Philadelphy, Horst; Fridrich, L.; Klima, G.; Margreiter, Raimund

doi:10.1016/0016-5085(92)90109-c

Cited by 42 publications

(5 citation statements)

References 18 publications

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“…Activated endothelial cells in turn would release adhesion molecules, facilitating hepatocyte anchorage. Moreover, induction of xanthine oxidase-mediated release of reactive oxygen species and superoxide radical production could lead to endothelial injury, paving the way for transplanted cells to enter the liver plate after approximately 16 to 20 hours of transplantation. Endothelial disruption is facilitated by the release of vascular endothelial growth factor (VEGFIVPF) from transplanted as well as host hepatocytes prior to the entry of cells into liver plates.…”

Section: Mechanisms Of Cell Engraftment and Proliferation In The Disementioning

confidence: 99%

Mechanisms of Cell Engraftment During Liver Repopulation with Hepatocyte Transplantation

1999

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Hepatocyte transplantation has excited much interest among investigators for applications in cell therapy and studies of fundamental mechanisms concerning liver biology. Progress in these areas has been greatly facilitated by the development of novel animal models. An understanding of early events during engraftment of transplanted cells is essential for optimal repopulation of the liver. Insights into how transplanted cells integrate in the parenchyma of the liver with reconstitution of specific plasma membrane structures is critical in devising therapeutic strategies for specific disorders. Moreover, these insights are necessary for understanding mechanisms concerning regulation of cell proliferation and gene expression in transplanted hepatocytes. Finally, analysis of the safety of cell transplantation is necessary for clinical applications of liver repopulation with cell transplantation. This review highlights selected advances in related areas concerning liver repopulation.

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Section: Mechanisms Of Cell Engraftment and Proliferation In The Disementioning

confidence: 99%

Mechanisms of Cell Engraftment During Liver Repopulation with Hepatocyte Transplantation

1999

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“…Among these procedures is the transplantation of isolated hepatocytes to various systemic sites. Numerous sites have been examined, including fat pads, muscle, subcutaneous tissue, peritoneum, lungs, kidney, liver, and spleen (Balladur et al 1994; Fuller 1988; Rivas et al 1992; Sandbichler et al 1992; Selden et al 1991). In spite of the disadvantages of the intrasplenic site, such as the need for syngeneic hepatocytes, which would otherwise require immunosuppressive therapy, and its size limitation, which allows the spleen to accommodate hepatic tissue amounting to only about 3–4% of normal liver weight, the spleen has been shown to be a highly effective site, at least in experimental animals (Dixit 1995).…”

Section: Introductionmentioning

confidence: 99%

Intrasplenic Transplantation of Isolated Adult Rat Hepatocytes

2011

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Adult male and female rat hepatocytes were individually transplanted into the spleens of adult male and female rats. The recipients were euthanized at either 8, 16, 30 or 45 weeks following transplantation at which time hepatic and splenic levels of liver-specific rat albumin mRNA as well as sex-dependent transcript levels of CYP2C11, -2C12, -2C7, -2A1 and -3A2, comprising >60% of the total concentration of hepatic constituent cytochrome P450, were determined. Whereas the pre-infused hepatocytes expressed their expected cytochrome P450 sexual dimorphisms (female-specific CYP2C12, male-specific CYP3A2 and female-predominant CYP2A1), their post transplantational competence now reflected the sexual dimorphisms of the recipient (as observed in the host’s liver) supporting the concept that the sex-dependent growth hormone circulating profiles are the determinants regulating the expression levels of hepatic cytochromes P450. Also expressed at normal concentrations in the pre-infused hepatocytes, male-specific CYP2C11 and female-predominant CYP2C7 were inexplicably undetectable in the spleens of both recipient males and females, irrespective of the sex of the donor hepatocytes, almost one year after transplantation.

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“…Nonetheless, in animals with liver failure induced by 90% hepatectomy, transplantation of microcarrier‐attached hepatocytes into the peritoneal cavity, subsequent to the onset of liver failure, did not alter survival21, although others showed that transplantation of microencapsulated hepatocytes improved survival16, 22–24. Transplantation of hepatocytes into lungs has also been reported to improve survival in acute liver failure25. However, the survival of hepatocytes in pulmonary capillaries is extremely limited and transplanted cells are cleared rapidly26, suggesting the possibility of alternative mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Integration and proliferation of transplanted cells in hepatic parenchyma followingD-galactosamine-induced acute injury in F344 rats

Gupta¹,

Rajvanshi²,

Irani³

et al. 2000

J. Pathol.

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Hepatocellular transplantation into the lung for temporary support of acute liver failure in the rat

Cited by 42 publications

References 18 publications

Mechanisms of Cell Engraftment During Liver Repopulation with Hepatocyte Transplantation

Mechanisms of Cell Engraftment During Liver Repopulation with Hepatocyte Transplantation

Intrasplenic Transplantation of Isolated Adult Rat Hepatocytes

Integration and proliferation of transplanted cells in hepatic parenchyma followingD-galactosamine-induced acute injury in F344 rats

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