Hepatocyte apoptotic index and p53 expression in obstructive jaundice rats

Abstract

Objective: Obstructive jaundice is a serious disease. It can detoriate critical functions in the liver. Material and method: A total of 20 male Wistar-Albino rats were randomly allocated into two groups consisting of ten in each as follows: Group I (Control) was subjected to a sham operation isolating the bile duct. Group II(Study) was subjected to acute cholestasis induced by bile duct ligation with 4/0 silk suture from two different places and full fold cut between ligatures. On the 7th day, group II rats were re-operated for liver sampling and sacrifi cation-aimed histological analysis through the old incision with anaesthesia. Hepatic tissues were histologically and immunohistochemically processed. The number of apoptotic and p53(+) cells were measured. Results: On the 7th day, the averages of direct and indirect bilirubin values in Group II rats were found to be 6.99 and11.70 mg/dl, respectively. They were observed to be statistically signifi cant. In the immunohistochemical evaluation p53 expression in hepatocytes was assessed, p53-positive hepatocytes were determined to exist quite widely in the tissue samples taken from the livers of rats in the study group. Nevertheless, no cells exhibiting p53 expression were found in the tissue samples of the control group. Conclusion: Apoptosis is a closed box and it might make it possible to stop the many disesase processes or accelerate the healing. If the principal effective mechanism in the liver under a certain stress factor is apoptosis, it is defi nite that it will make a difference in the treatment approach. Consequently, we can say that both apoptotic index and p53 expression increase in the rats' liver with biliary obstruction (Fig. 4, Ref. 51). Text in PDF www.elis.sk.

Bile acids and bilirubin effects on osteoblastic gene profile. Implications in the pathogenesis of osteoporosis in liver diseases

“…Both phenomena occur after only few hours of treatment, suggesting that bilirubin could play a major role in the regulation of the apoptotic related genes. This observation leads us to confirm our earlier observations about the pro-apoptotic role of bilirubin on osteoblastic cells [24] and other cell types and tissues [15][16]25,26]. Likewise, the relationship between LCA and apoptosis in different tissues and cell types has been widely demonstrated.…”

“…Bilirubin (Sigma) stock solution of 1600 M was prepared just before use by dissolving bilirubin in 10 ml 0.01N NaOH under dim light as previously described [12,[15][16][17][18]. It was filtered through a sterile filter (0.22 m pore size) and adjusted to pH 7.2-7.4 with 0.1N HCl, if necessary.…”

The Effect of Montelukast on Liver Damage in an Experimental Obstructive Jaundice Model

“…The experimental obstructive jaundice model used in the present study is an irreversible obstruction model which does not fully simulate the clinical course of obstructive jaundice in humans. However, this model is widely used in the literature for the evaluation of pathophysiology and the treatment of experimental obstructive jaundice [ 31 , 32 , 33 , 34 ]. Thus, we preferred to use this model in the current study.…”

Yinchenhao decoction attenuates obstructive jaundice-induced liver injury and hepatocyte apoptosis by suppressing protein kinase RNA-like endoplasmic reticulum kinase-induced pathway

“…Apoptosis is a programmed mechanism of cell death caused by various physiological and pathological conditions characterized by DNA damage[4]. In 2014, Kosar et al[5] confirmed that serious cellular damage occurs in hepatocytes during OJ. If DNA damage in a cell cannot be resolved, apoptotic signaling mechanisms can be activated, forcing the cell to self-destruct[6].…”