Hepatocyte transplantation for total liver repopulation

Abstract

Hepatocyte transplantation (HT) is an attractive therapeutic alternative to liver transplantation. A number of experiments have shown the feasibility of total liver parenchymal cell replacement by transplanted hepatocytes. In this review, we would like to highlight researches and clinical reports of HT for liver repopulation. Cellular source of clinical HT should be safety. Immortalized cells, hepatic stem cells, and other stem cells have been used for an experimental model for HT. The exact mechanism of the cell engraftment after HT has not been completely understood, although there were some markers to detect and investigate transplanted cells. In order to achieve liver repopulation following HT, a mild hepatic damage may need to facilitate cell engraftment and replace the host liver by transplanted cells. Hormonal factor may use for the same purpose. Despite the results of preclinical studies promising clinical benefits for cell therapy, the clinical experience of HT has been disappointing, except in a few cases. HT may become an alternative for liver transplantation in the future; however, many efforts should made before establishing an effective method for HT and liver replacement therapy.

Serum-derived hepatitis C virus infectivity in interferon regulatory factor-7-suppressed human primary hepatocytes

“…When cultured in vitro, however, they proliferate poorly and divide only a few times [10]. Continuous proliferation could be achieved however by introducing oncogenes, such as Simian virus large tumor antigen (SV40 T) [11]. This often resulted in tumor development [12] together with numerical (aneuploidy) and structural (aberrations) chromosome abnormalities [13].…”

Zonal expression of hepatocytic marker enzymes during liver repopulation

“…So far, animal transplantation models oVer unique opportunities to investigate the mechanisms of both the integration and interaction of transplanted hepatocytes with host liver cells (Mizuguchi et al 2005). Hepatocytes are metabolically specialised cells displaying distinctive gene expression patterns within the liver lobule.…”

Autologous Serum Improves Yield and Metabolic Capacity of Monocyte-Derived Hepatocyte-Like Cells: Possible Implication for Cell Transplantation

“…One likely reason during the dedifferentiation step could be simply Hepatocyte transplantation is a promising alternative to whole-organ transplantation either to bridge patients described by a graft versus host reaction (7,11) of the cells towards the applied human type AB sera or FCS. awaiting a donor liver or to correct at least temporarily genetic deficiencies of the liver (19,27,29,33,34,38,40).…”