Hepatoma-derived growth factor stimulates smooth muscle cell growth and is expressed in vascular development

Lobe, David R.; Matsumura, Martin E.; Nakamura, Hitomi; McNamara, Coleen A.

doi:10.1172/jci7497

Cited by 122 publications

(146 citation statements)

References 17 publications

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“…3A). As shown in Figure 3, the HDGF cDNA hybridized to a single 2.3-kb band as reported previously (Everett et al, 2000;Nakaumra et al, 1994). As expected, HDGF expression was much higher in the conotruncus as compared to the newborn aorta.…”

Section: Hdgf Expression In the Developing Ratsupporting

confidence: 80%

“…Consistent with the evidence that HDGF is a secreted protein, in vitro studies have shown HDGF to be an exogenous mitogen for HuH-7 (Nakamura et al, 1994), COS-7 (Nakamura et al, 1994), aortic vascular smooth muscle (Everett et al, 2000), and endothelial cells (Oliver and Al-Awqati, 1998). HDGF may play an important role in vascular growth as we recently identified HDGF as a potent mitogen for vascular smooth muscle cells in vitro with re-expression in response to vascular injury in pathologic animal models and in human atherosclerotic vascular disease (Everett et al, 2000).…”

Section: Introductionmentioning

confidence: 69%

“…We have previously shown that HDGF is re-expressed in vascular smooth muscle cells in response to injury and that this expression of HDGF was linked to cell proliferation (Everett et al, 2000). Furthermore, HDGF is a mitogen for endothelial cells (Oliver and Al-Awqati, 1998) and vascular smooth muscle cells (Everett et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, HDGF is a mitogen for endothelial cells (Oliver and Al-Awqati, 1998) and vascular smooth muscle cells (Everett et al, 2000). However, HDGF may play other roles such as regulating cell fate or differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…A polyclonal HDGF antibody previously shown to detect HDGF (Everett et al, 2000) was used in an immunocytochemical assay to map the cellular expression of HDGF in the developing rat cardiovascular Fig. 1.…”

Section: Cellular Mapping Of Hdgf Expression In the Developing Rat Camentioning

confidence: 99%

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Identification, cloning, and developmental expression of hepatoma‐derived growth factor in the developing rat heart

Everett

2001

Developmental Dynamics

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Hepatoma derived growth factor (HDGF) was identified as a developmentally regulated cardiac gene by mRNA differential display using 12-day rat fetal conotruncus vs. newborn aorta. The full-length rat HDGF cDNA was cloned from a rat fetal heart cDNA library and found to be 94 and 88% homologous to the mouse and human sequence, respectively. The rat sequence, like the human and mouse, contains a highly conserved amino portion and putative bipartite nuclear localization sequence. By Northern analysis, HDGF is highly expressed in the fetal conotruncus, heart, kidney, brain, and gut. By immunocytochemistry, HDGF was first detected only in atrial myocytes, hind gut epithelia, and notochord of the E10 rat with a nuclear expression pattern. By E12, expression had broadened to include the ventricular myocytes, endocardial cells, and cells of the ventricular outflow tract. HDGF is unique in that it is the first described nuclear targeted growth factor in the developing heart. The early expression of HDGF in embryonic heart and fetal gut suggests that HDGF may play a role in cardiovascular growth and differentiation.

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Section: Hdgf Expression In the Developing Ratsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Cellular Mapping Of Hdgf Expression In the Developing Rat Camentioning

confidence: 99%

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Identification, cloning, and developmental expression of hepatoma‐derived growth factor in the developing rat heart

Everett

2001

Developmental Dynamics

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Expression of hepatoma‐derived growth factor in hepatocellular carcinoma

Huang

Liu

et al. 2003

Cancer

110

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The design of a broad application tumor vaccine requires the identification of tumor antigens expressed in a majority of tumors of various origins. We questioned whether the major stress‐inducible heat shock protein Hsp70 (also known as Hsp72), a protein frequently overexpressed in human tumors of various histological origins, but not in most physiological normal tissues, constitutes a tumor antigen. We selected the p391 and p393 peptides from the sequence of the human inducible Hsp70 that had a high affinity for HLA‐A*0201. These peptides were able to trigger a CTL response in vivo in HLA‐A*0201‐transgenic HHD mice and in vitro in HLA‐A*0201+ healthy donors. p391‐ and p393‐specific human and murine CTL recognized human tumor cells overexpressing Hsp70 in a HLA‐A*0201‐restricted manner. Tetramer analysis of TILs showed that these Hsp70 epitopes are targets of an immune response in many HLA‐A*0201+ breast cancer patients. Hsp70 is a tumor antigen and the Hsp70‐derived peptides p391 and p393 could be used to raise a cytotoxic response against tumors of various origins. © 2003 Wiley‐Liss, Inc.

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Hepatoma‐derived growth factor (HDGF) is dispensable for normal mouse development

Gallitzendoerfer¹,

Abouzied²,

Hartmann³

et al. 2008

Developmental Dynamics

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Hepatoma-derived growth factor (HDGF) is suggested to be involved in organ development and exhibits proliferative, angiogenic, and neurotrophic activity. The in vivo functions are, however, so far unknown. In this study, we generated HDGF-deficient mice, in which parts of the HDGF gene were replaced by a gene encoding green fluorescent protein (eGFP). HDGF؊/؊ mice are viable with no apparent morphological abnormalities. Cultured HDGF-deficient dermal fibroblasts show unaltered proliferation rates and cellcycle distributions. In contrast to previous studies, our data demonstrate that signal pathways involved in the response to extracellular HDGF do not depend on the presence of intracellular HDGF. Contrary to the reported role of HDGF as a modulator of apoptosis, similar apoptotic rates were found between wild-type and HDGF-deficient fibroblasts following tumor necrosis factor ␣ (TNF␣) -induced apoptosis or cellular stress. The lack of obvious biochemical and morphological phenotypes in HDGF-deficient mice demonstrates that in vivo HDGF is dispensable for normal development in mice.

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Hepatoma-derived growth factor stimulates smooth muscle cell growth and is expressed in vascular development

Cited by 122 publications

References 17 publications

Identification, cloning, and developmental expression of hepatoma‐derived growth factor in the developing rat heart

Identification, cloning, and developmental expression of hepatoma‐derived growth factor in the developing rat heart

Expression of hepatoma‐derived growth factor in hepatocellular carcinoma

Hepatoma‐derived growth factor (HDGF) is dispensable for normal mouse development

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