Hepatopancreas transcriptome alterations in red claw crayfish (Cherax quadricarinatus) under microcystin-LR (MC-LR) stress

Lu, Yao-Peng; Zheng, Ping; Zhang, Ze-Long; Zhang, Xiuxia; Li, Juntao; Wang, Dongmei; Xu, Jia-Rui; Xian, Jian-An; Wang, An‐Li

doi:10.1016/j.aqrep.2023.101478

Cited by 3 publications

(4 citation statements)

References 79 publications

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“…It plays a crucial role in controlling metabolism, transcription, cell division, and cell death in crustaceans. ALF, an integral component of AMPs, involves in the first-line host defense mechanism of the innate immune response . Previous studies have shown that ALF5 and other immune factors in mud crab (Scylla paramamosain) are upregulated after stimulation with Vibrio parahemolyticus .…”

Section: Resultsmentioning

confidence: 99%

“…ALF, an integral component of AMPs, involves in the first-line host defense mechanism of the innate immune response. 28 Previous studies have shown that ALF5 and other immune factors in mud crab (Scylla paramamosain) are upregulated after stimulation with Vibrio parahemolyticus. 29 Legumain was reported as a downregulated gene in white shrimp (Litopenaeus vannamei) when exposed to cold stress.…”

Section: Validation Of Tmt-based Proteomics Approachesmentioning

confidence: 99%

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Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

Du,

Wu,

Liao

et al. 2024

J. Agric. Food Chem.

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Physicochemical properties and protein alterations in Ovalipes punctatus during cold-chain transportation were examined via sensory scores, water-holding capacity (WHC), glucose (GLU) content, catalase (CAT) activity, urea nitrogen (UN) content, and tandem mass tag (TMT)-based proteomic analysis. The results revealed that sensory characteristics and texture of crab muscle deteriorated during transportation. Proteomic analysis revealed 442 and 470 different expressed proteins (DEPs) in crabs after 18 h (FC) and 36 h (DC) of transportation compared with live crabs (LC). Proteins related to muscle structure and amino acid metabolism significantly changed, as evidenced by the decreased WHC and sensory scores of crab muscle. Glycolysis, calcium signaling, and peroxisome pathways were upregulated in the FC/LC comparison, aligning with the changes in GLU content and CAT activity, revealing the stress response of energy metabolism and immune response in crabs during 0−18 h of transportation. The downregulated tricarboxylic acid (TCA) cycle and carcinogenesis-reactive oxygen species pathways were correlated with the decreasing trend in CAT activity, suggesting a gradual retardation in both energy and antioxidant metabolism in crabs during 18−36 h of transportation. Furthermore, the regulated purine nucleoside metabolic and nucleoside diphosphate-related processes, with the increasing changes in UN content, revealed the accumulation of metabolites in crabs.

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Section: Resultsmentioning

confidence: 99%

Section: Validation Of Tmt-based Proteomics Approachesmentioning

confidence: 99%

Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

Du,

Wu,

Liao

et al. 2024

J. Agric. Food Chem.

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“…SSRs and SNPs are essential molecular markers in the fields of genetic diversity analysis and molecular-assisted breeding, among others [96,97]. In recent years, they have been applied to the study of genetic variation in numerous crustacean species [98][99][100]. However, there have been no reports of SSRs or SNPs identified in the hepatopancreas of M. rosenbergi under Cu exposure.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Zhang,

Bao,

Guo

et al. 2024

Animals

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The contamination of aquatic ecosystems by the heavy metal copper (Cu) is an important environmental issue and poses significant risks to the physiological functions of aquatic organisms. Macrobrachium rosenbergii is one of the most important freshwater-cultured prawns in the world. The hepatopancreas of crustaceans is a key organ for immune defense, heavy metal accumulation, and detoxification, playing a pivotal role in toxicological research. However, research on the molecular response of the hepatopancreas in M. rosenbergii to Cu exposure is still lacking. In this study, the transcriptomic response in the hepatopancreas of M. rosenbergii was studied after Cu exposure for 3 and 48 h. Compared with the control group, 11,164 (7288 up-regulated and 3876 down-regulated genes) and 10,937 (6630 up-regulated and 4307 down-regulated genes) differentially expressed genes (DEGs) were identified after 3 and 48 h exposure, respectively. Most of these DEGs were up-regulated, implying that gene expressions were largely induced by Cu. Functional enrichment analysis of these DEGs revealed that immunity, copper homeostasis, detoxification, DNA damage repair, and apoptosis were differentially regulated by Cu. Seven genes involved in immunity, detoxification, and metabolism were selected for validation by qRT-PCR, and the results confirmed the reliability of RNA-Seq. All these findings suggest that M. rosenbergii attempts to resist the toxicity of Cu by up-regulating the expression of genes related to immunity, metabolism, and detoxification. However, with the excessive accumulation of reactive oxygen species (ROS), the antioxidant enzyme system was destroyed. As a result, DNA damage repair and the cellular stress response were inhibited, thereby exacerbating cell damage. In order to maintain the normal function of the hepatopancreas, M. rosenbergii removes damaged cells by activating the apoptosis mechanism. Our study not only facilitates an understanding of the molecular response mechanisms of M. rosenbergii underlying Cu toxicity effects but also helps us to identify potential biomarkers associated with the stress response in other crustaceans.

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“…The hepatopancreas is a vital organ in C. fluminea that plays a crucial role in digestion, nutrient storage, and metabolism [12][13][14]. Therefore, it is an essential target for investigating the effects of NP and MC-LR stress.…”

Section: Introductionmentioning

confidence: 99%

Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

Zhang,

Wang,

Liu

et al. 2023

Water

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In agricultural and fishery production, the occurrence of cyanobacterial blooms and the contamination of freshwater systems with microplastics have become increasingly important research focuses. However, the individual and combined toxic mechanisms of these two pollutants are not yet fully understood. Therefore, in this study, we analyzed the effects of these two stressors, microcystins (MC) and nanoplastics (NP), on the transcriptome and metabolome of the hepatopancreas of river clams. RNA and metabolites extracted from river clams treated with MC, NP, and a combination of MC and NP were used to construct standardized cDNA libraries, which were then subjected to integrated analysis. Significant enrichment of 49 pathways, 34 pathways, and 44 pathways was observed in the MC group compared to the control group, NP group compared to the control group, and NP-MC group compared to the control group, respectively. In these three experimental groups, we found that the lysosome pathway, which affects immune function and cell apoptosis, was enriched with numerous differentially expressed genes and metabolites. Changes in ATP6N and ADP may impair lysosomal acidification and disrupt normal lysosomal degradation processes, indicating interference with the hepatopancreatic metabolism of pollutants. Interestingly, we observed significant alterations in the cathepsin family, and the downregulation of cathepsin genes, along with the downregulation of ATP6N, implies a potential disruption in lysosomal proteolysis. In the NP-MC group, the downregulation of purine expression levels suggests an impact on the immune system of river clams by NP-MC. In conclusion, while there is some overlap in the damage caused to the hepatopancreas of river clams by MCs, MPs, and the combination of NP-MC, further research is necessary to fully understand their effects.

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Hepatopancreas transcriptome alterations in red claw crayfish (Cherax quadricarinatus) under microcystin-LR (MC-LR) stress

Cited by 3 publications

References 79 publications

Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

Comparative Transcriptome Analysis of the Hepatopancreas from Macrobrachium rosenbergii Exposed to the Heavy Metal Copper

Combining Transcriptomic and Metabolomic Analyses to Investigate the Acute Effects of Microcystin-LR and Nanoplastics of Asian Clams

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