Hepatopancreatic metabolomics shedding light on the mechanism underlying unsynchronized growth in giant freshwater prawn, Macrobrachium rosenbergii

Abstract

The giant freshwater prawn, Macrobrachium rosenbergii (M. rosenbergii) as an important freshwater aquaculture species with high commercial value, exhibited unsynchronized growth. However, the potentially metabolic mechanism remains unclear. In this study, we used liquid chromatography tandem mass spectrometry (LC-MS/MS) to investigate the hepatopancreatic metabolic profiles of twenty giant freshwater prawns between the fast-growing group and slow-growing group. In the metabolomics assay, we isolated 8,293 peaks in positive and negative iron mode. Subsequently, 44 significantly differential metabolites were identified. Functional pathway analysis revealed that these metabolites were significantly enriched in three key metabolic pathways. Further integrated analysis indicated that glycerophospholipid metabolism and aminoacyl-tRNA biosynthesis have significant impact on growth performance in M.rosenbergii. Our findings presented here demonstrated the critical metabolites and metabolic pathways involved in growth performance, moreover provided strong evidence for elucidating the potentially metabolic mechanism of the unsynchronized growth in M. rosenbergii.

Effect of Two Kinds of Fertilizers on Growth and Rhizosphere Soil Properties of Bayberry with Decline Disease

“…Among the ten obtained pathways, CF caused significant change in seven metabolic pathways, including aminoacyl-tRNA biosynthesis, C5-branched dibasic acid metabolism, galactose metabolism, cyanoamino acid metabolism, phenylalanine metabolism, bacterial chemotaxis, and pyrim-idine metabolism (Figure 8). The aminoacyl-tRNA biosynthesis is one essential for protein biosynthesis, and has significant impact on growth performance in Macrobrachium rosenbergii [60]. In higher plants, the amino acid phenylalanine is a substrate of both primary and secondary metabolic pathways.…”

Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)

“…Growth rate is a key factor to associate with the yield and economic benefits for the crustacean farming industry. However, for most crustacean species, the growth is unsynchronized, which means even cultured under the same genetic and environmental conditions (Ren et al, 2017;Jiang et al, 2020;Uengwetwanit et al, 2020), individual crustacean grows at different rates. The unsynchronized growth phenomenon expands the total harvest time, leads to more management efforts and higher farming cost (Hao et al, 2019;Jiang et al, 2020).…”

“…However, for most crustacean species, the growth is unsynchronized, which means even cultured under the same genetic and environmental conditions (Ren et al, 2017;Jiang et al, 2020;Uengwetwanit et al, 2020), individual crustacean grows at different rates. The unsynchronized growth phenomenon expands the total harvest time, leads to more management efforts and higher farming cost (Hao et al, 2019;Jiang et al, 2020). With more and more large-scale farming models being developed in recent years, how to efficiently minimize the unsynchronized growth becomes a critical question for the industry.…”