Hepatoprotective activity of aqueous and ethanolic Bixa orellana L. leaf extracts against carbon tetrachloride-induced hepatotoxicity

Abstract

Background: There is an increasing incidence of liver failure and lack of effective drugs for liver diseases. Aim and Objective: This study identified the phytochemicals present in aqueous and ethanolic Bixa orellana L. leaf extracts and investigated the hepatoprotective activity against carbon tetrachloride (CCl 4 )-induced hepatotoxicity in mice. Specifically, the study compared serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and examined liver tissues histopathology. Materials and Methods: Aqueous and ethanolic B. orellana L. leaf extracts were prepared and screened for phytochemical contents. An oral dose of 500 mg/kg body weight was identified from toxicity test and administered to albino mice. Microscopic examination of the liver tissues assessed the extent of hepatic injury. Serum AST and ALT levels were compared using one-way analysis of variance with Bonferroni post hoc analysis at 5% level of significance using Stata/SE V12.0 software. Results: B. orellana L. leaves contained alkaloids, anthraquinones, sugars, and tannins. Aqueous and ethanolic leaf extracts of B. orellana L. did not show any toxicity up to 2000 mg/kg body weight oral dose in mice. Pre-treatment for 7 days before CCl 4 administration significantly prevented elevation of serum AST and ALT levels with histopathologic findings showing a protective effect on the hepatocytes. Conclusion: B. orellana L. leaves have potent hepatoprotective activity against oxidative damage.

Phytochemistry and pharmacological activity of the genus artemisia

“…Hydroalcoholic extract of A. dracunculus reinstated the elevated serum enzyme extents, reduced liver antioxidant markers, strived, and proficient antioxidant activity under in-vitro situations demonstrating that it has hepatoprotective antioxidant efficiencies in CCl 4 -intoxicated rats (Mankani et al 2005;Gilani and Janbaz 1993). The hepatoprotective property of hydro-alcoholic extracts of aerial parts of A. dracunculus (HAAD) caused by extenuated lipid peroxidation improved protection of the hepatocytes against reactive oxygen species (ROS) (Lopez et al 2017). The histopathological reports also proved the effectiveness of the plant extract.…”

Study The protective Effects of Cichorium intybus L. Root Extract Against Carbon Tetrachloride-Induced Hepatotoxicity in Broiler Chickens

“…In the broiler chicken industry, it is indispensable to monitor and manage of the oxidative stress because this event can directly affect the rate of production and increase the mortality rate of chicks [15]. Several investigations announced the administration of natural medicinal plants to control and decrease the adverse effects of oxidative stress on hepatic functions [16,17].…”

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“…Its leaves have antiseptic, antibacterial, and antiemetic effects, whereas the seeds were used for fever, buccal tumors, jaundice. Many pharmacologic studies have been conducted on its potential as a source of medicine 13 . B. orellana was shown activity against protozoan, helminths and had platelet anti-aggregant activity 14 .…”