Hepatoprotective Activity of Herbal Preparation (Hp-4) against Alcohol Induced Hepatotoxicity in Mice

Abstract

Free radicals include both Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS).When free radicals are produced in a regulated manner in a healthy human body it is scavenged efficiently by antioxidant defense system. But excess generation of pro-oxidants by continuous chain reaction in the form of ROS and RNS cause several human diseases. The shift of the balance in the favour of pro-oxidants results in a condition called “oxidative stress”. Alcohol is primarily metabolized in the liver to generate ROS and RNS, leading to diseases such as cirrhosis, fatty liver and chronic hepatitis. Alcohol induced damage is associated with oxidative stress. The excess generation of prooxidants and reduced antioxidant levels provide an effective model of Hepatotoxicity which is noteworthy. Recent trend is to discover polyherbal formulation of medicinal plants which have hepatoprotective function. In the present study 80% alcoholic extract of leaves of Aloe vera, Bacopa monniera, Moringa oleifera and rhizome of Zingiber officinale were utilized to prepare Herbal Preparation or HP-4.Further the hepatoprotective effects of HP-4 was tested in alcohol induced Hepatotoxicity in mice. Silymarin is a well known hepatoprotective drug was used as a standard for comparison. Biochemical and histopathological studies provided ample evidence that HP-4 provided a hepatoprotective role in alcohol induced hepatotoxicity which was comparable to drug Silymarin. The presence of phytochemicals in HP-4 provided a synergistic, supra-additive and co-operative effects in the hepatoprotective function in alcohol induced hepatotoxicity mice model. DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9346 Int J Appl Sci Biotechnol, Vol 2(1): 50-58

Identification of the Therapeutic Effects of Gamma-Irradiated Chamomile Aqueous Extract Against Alcohol Induced Hepato-nephrotoxicity in Rats

“…These results are in agreement with Padmanabhan and Jangle (2014) who illustrated that alcohol feeding induces increases in serum activities of AST, ALT, ALP, GGT and LDH enzymes which are markers of liver damage. Alcohol intoxication, as a result of structural changes leads to a significant increase in the cell membrane permeability to ions.…”

Protective Effects of Moringa oleifera extract on Isoniazid and Rifampicin Induced Hepatotoxicity in Rats: Involvment of Adiponectin and Tumor Necrosis Factor-α

“…In current study, silymarin administration elevated the activity of GPx compared to the hepatotoxic rats, similar to the study previously mentioned by Padmanabhan and Jangle [55].…”

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“…The DPPH ,nitric oxide and hydroxyl radical scavenging activity The total antioxidant activity by FRAP assay, reducing property was determined, by the methods described by authors Padmanabhan and Jangle; 2012 7 "b" . The anti lipid peroxidation of wheat grass juice was carried out in mouse liver as well as in brain homogenate of D-galactosamine induced hepatic damage (Padmanabhan and Jangle ;2014) 8 The 10% liver homogenate was centrifuged at 3000 rpm for 10 minutes and the supernatant obtained was used. 0.5 ml of the supernatant obtained from liver homogenate was added to 0.5 ml saline in a series of test tubes to which wheat grass juice was added in concentration ranging from 50μg/ml to 250μg/ml.…”

“…The test tubes were allowed to cooled and thereafter centrifuged and the absorbances of the supernatants were measured on spectrophotometer at 530 nm. 8 , antioxidant…”