Hepatoprotective activity of Lepidium sativum seeds against D-galactosamine/lipopolysaccharide induced hepatotoxicity in animal model

Abstract

BackgroundFulminant hepatic failure (FHF) is clinical syndrome with very poor prognosis and high mortality there is urgent need for the development of safe and non-toxic hepatoprotective agents for the adequate management of hepatitis. Hepatoprotective effect of the Lepidium sativum ethanolic extract (LSEE) was assessed by D-galactosamine-induced/lipopolysaccharide (400 mg/kg and 30 μg/kg) liver damage model in rats.MethodsHepatoprotective activity of LSEE (150 and 300 mg/kg) and silymarin on D-GalN/LPS induced FHF in rat was assessed using several liver function enzyme parameters. Antioxidant properties as antioxidant stress enzymes were assessed in hepatic Liver as well as mRNA expression of cytokines genes such as TNF-α, IL-6, and IL-10 and stress related genes iNOS and HO-1 were determined by RT-PCR. Protein expression of apoptotic genes were evaluated through western blot. MPO and NF-κB DNA-binding activity was analyzed by ELISA. The magnitude of hepatic impairment was investigated through histopathological evaluation.ResultsMarked amelioration of hepatic injuries by attenuation of serum and lipid peroxidation has been observed as comparable with silymarin (25 mg/kg p.o). D-GalN/LPS induced significant decrease in oxidative stress markers protein level, and albumin. LSEE significantly down-regulated the D-GalN/LPS induced pro-inflammatory cytokines TNFα and IL-6 mRNA expression in dose dependent fashion about 0.47 and 0.26 fold and up-regulates the IL-10 by 1.9 and 2.8 fold, respectively. While encourages hepatoprotective activity by down-regulating mRNA expression of iNOS and HO-1. MPO activity and NF-κB DNA-binding effect significantly increased and was mitigated by LSEE in a dose-dependent style as paralleled with silymarin.ConclusionOur data suggests that pretreatment of LSEE down regulates the caspase 3 and up-regulates the BCl2 protein expression. The above findings revealed that Lepidium sativum has significant hepatoprotective activity.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1483-4) contains supplementary material, which is available to authorized users.

Anti-glycation study of hydro-alcohol and aqueous extracts of Moroccan plant species

“…These compounds were extracted from plants selected because of their traditional use in medicine as an ingredient of many polyherbal formulations for the treatment of several pathologies (Baharvand-Ahmadi B et al, 2015;Pusadkar P.P. et al, 2015;Rahmani A.H. and Aly S.M., 2015;Bahmani M et al, 2016;Raish M et al, 2016;Vardapetyan H et al, 2016;Özcan M.M. and Matthäus B, 2017;Sim M.O.…”

S‐allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/d‐galactosamine in mouse: Underlying mechanisms

“…Apoptosis also elevates subsequent to LPS/D-Gal challenge in the liver. [65,66] In this regard, it has been proved that LPS-D/Gal enhances caspase 3 and upregulates expression of BCl2 [67] and raises DNA fragmentation. [68] On the contrary, SAC (100 mg/kg) administration significantly reduced these apoptotic indices.…”

Phytoconstituents, Pharmacological Activity, and Medicinal Use of Lepidium Sativum Linn.: A Review

“…Thus, significantly alleviate the reduction of lipid peroxidation and restored the antioxidant enzymes and total protein to normal levels. LSEE decreases hepatic injuries and structural damage through the decline of oxidative stress, inflammation, and apoptosis in the liver [44].…”