Hepatoprotective activity of <i> Vitex trifolia</i> against carbon tetrachloride-induced hepatic damage

Abstract

Aqueous and ethanol extracts of leaf of Vitex trifolia was investigated for hepatoprotective activity against carbon tetrachloride induced liver damage. To assess the hepatoprotective activity of the extracts, various biochemical parameters viz., total bilirubin, total protein, alanine transaminase, aspartate transaminase and alkaline phosphatase activities were determined. Results of the serum biochemical estimations revealed significant reduction in total bilirubin and serum marker enzymes and increase in total protein in the animals treated with ethanol and aqueous extracts. However significant rise in these serum enzymes and decrease in total protein level was noticed in CCl4 treated group indicating the hepatic damage. The hepatoprotective activity is also supported by histological studies of liver tissue. Histology of the liver tissue treated with ethanol and aqueous extracts showed normal hepatic architecture with few fatty lobules. Hence the present study revealed that Vitex trifolia could afford significant protection against CCl4 induced hepatocellular injury.

Hepatoprotective Role of Ethanolic Extract ofVitex negundoin Thioacetamide-Induced Liver Fibrosis in Male Rats

“…Concurrently, the effect of SY on liver cells suggests that the hepatoprotective action is due to the presence of flavonoids and phenols and their regenerative ability and its antioxidant effects [37, 38]. …”

Traditional phytotherapy remedies used in Southern Rwanda for the treatment of liver diseases

“…Using various in vivo and in vitro models, some of the plants used in Rwanda, or other species from the same genus, have been previously investigated against an hepatotoxicity induced (i) by CCl 4 in rats and/or mice (Hypoestes triflora, Acanthus ilicifolius, Solanum nigrum, Leucas hirta, Indigofera sp., Myrica rubra) (Van Puyvelde et al, 1989;Babu et al, 2001;Singh et al, 2001;Manjunatha et al, 2005;Shahjahan et al, 2005;Singh et al, 2006;Lin et al, 2008;Mukherjee et al, 2009;Xu et al, 2009); (ii) by CCl 4 and dgalactosamine in rats (Ocimum gratissimum and Ocimum basilicum) (Lin et al, 1995); (iii) by CCl 4 and acetaminophen in rats and/or mice (Vernonia amygdalina, Carica papaya, Bidens pilosa) (Babalola et al, 2001;Rajkapoor et al, 2003;Iwalokun et al, 2006;Yuan et al, 2008); (iv) by acetaminophen and thioacetamide in rats (Hygrophila auriculata) (Singh and Handa, 1995;Shanmugasundaram and Venkataraman, 2006); (v) by CCl 4 , acetaminophen, ethanol, dgalactosamine, iron and Amanita phalloides in rats and mice (Silybum marianum) (Chih et al, 1996;Pradhan and Girish, 2006); and (vi) by CCl 4 and acetaminophen in guinea pigs and rats (Ocimum lamiifolium, Crassocephalum vitellinum, Guizotia scabra and Vernonia lasiopus) (Mukazayire et al, 2010). An antihepatitis C activity has also been demonstrated for Embelia schimperi that acts on the HCVProtease (Hussein et al, 2000).…”

A comprehensive review on herbal drugs for hepatoprotection of 21stCentury

“…In this review article, an attempt has been made to compile the reported hepatoprotective plants from India or abroad and may be useful to the health professionals, scientists and scholars working in the field of pharmacology and therapeutics to develop evidence-based alternative medicine to cure different kinds of liver diseases in men and animals. Vitex trifolia [82] Leaf Aqueous and ethanol Persicogenin, artemetin, luteolin, penduletin and chrysosplenol-D Decreases the rise of serum enzymes and total protein level in liver Vitis vinifera [83] Leaves Ethanol Halimane-type diterpenes, vitetrifolins…”