Hepatoprotective Activity of Methanol Extract of Melastoma malabathricum Leaf in Rats

Kamisan, Farah Hidayah; Yahya, Farhana; Ismail, Nurul Ain; Din, Syafawati Shamsahal; Mamat, Siti Syariah; Zabidi, Zalina; Zainulddin, Wan Noraziemah Wan; Mohtarrudin, Norhafizah; Husain, Hadijah; Ahmad, Zubair; Zakaria, Zainul Amiruddin

doi:10.1016/j.jams.2012.08.002

Cited by 24 publications

(11 citation statements)

References 11 publications

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“…[20] showed that the aqueous extract L. nepetifolia, given as chronic post-treatment, protected against CCl 4 -induced hepatic damage in rats in a similar manner. Additionally, several studies have shown that the methanol and aqueous extracts of some medicinal plants protect against APAPinduced hepatotoxicity by abrogating the drug-induced increase in liver enzymes and maintaining normal liver morphology [21][22][23][24]. Several studies have identified antioxidant phytochemicals, such as polyphenols and flavonoids, which were suggested to be responsible for the medicinal plants' protective properties against APAP-induced hepatic injury [25][26][27][28].…”

Section: Pre-treatment and Post-treatment Effects Of Extracts On Hepamentioning

confidence: 99%

Leonotis nepetifolia Protects against Acetaminophen-Induced Hepatotoxicity: Histological Studies and the Role of Antioxidant Enzymes

AF¹,

YN²

2016

Nat Prod Chem Res

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Aim of the study: High dose acetaminophen (APAP) increases the risk of liver injury caused by oxidative stress due to accumulation of reactive species. Although N-acetyl cysteine is the standard antidote used to treat acute APAPinduced liver failure, we proposed that known antioxidant phytochemicals in Leonotis nepetifolia extracts would protect against APAP-induced hepatic injury by modulating the activities of antioxidant enzymes. Materials and methods:Methanol and aqueous extracts of L. nepetifolia were orally administered in doses ranging (250 mg/kg to 1000 mg/kg) as pre-and post-treatment with high dose APAP (550 mg/kg) to Swiss albino mice. Twenty-four hours after the final dose, animals were euthanized and blood and liver collected for liver enzymes (ALT and AST), histological assessment and antioxidant enzyme assays.Results: Methanol and aqueous extracts as pre-treatment and post-treatment protected against hepatic injury. Extracts abrogated the 14-fold and 4-fold APAP-induced increases in ALT and AST respectively, including histopathological damage (p<0.05). Extracts reversed APAP-induced 4-fold and 14-fold increases in GR and SOD activities respectively (p<0.05). Additionally, extracts reversed APAP-induced decline in GPx activity; particularly the aqueous extract as pre-treatment increased GPx activity up to 2.2-fold over saline-treated controls (p<0.05). Conclusions:Extracts, as pre-treatment and post-treatment, prevented APAP-induced hepatic injury by modulating the activities of antioxidant enzymes. Of particular interest, is the reversal of APAP-induced decrease in GPx activity and increase in SOD activity? Extract-induced increase in GPx activity would facilitate the scavenging of hydroperoxide and peroxide reactive species generated by high dose APAP. We propose that antioxidant phytochemicals in L. nepetifolia may be acting as free radical scavengers which results in reduced SOD activity.

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Section: Pre-treatment and Post-treatment Effects Of Extracts On Hepamentioning

confidence: 99%

Leonotis nepetifolia Protects against Acetaminophen-Induced Hepatotoxicity: Histological Studies and the Role of Antioxidant Enzymes

AF¹,

YN²

2016

Nat Prod Chem Res

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“…Rats with carbon tetrachloride (CCl4)-induced liver injury were used to assess the hepatoprotective and antioxidant effect of MIE in vivo. The level or activity of alkaline phosphatase (ALP), glutamate pyruvate transaminase (ALT), aspartate aminotransferase (AST), and total bilirubin (TB) in the blood serum and thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) in the liver and kidney of the rats were assayed using standard procedures 48 . 40.…”

Section: Meconopsis Integrifoliamentioning

confidence: 99%

Untitled

2014

IJPSR

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Hepatic diseases (Liver diseases) are a major worldwide health problem, with high endemicity in developing countries. Hepatic disease is a term for a collection of conditions, diseases, and infections that affect the cells, tissues structures, or functions of the liver. Liver has a wide range of functions, including detoxification, protein synthesis, and production of biochemical necessary for digestion and synthesis as well as breakdown of small and complex molecules, many of which are necessary for normal vital functions. There are no specific allopathic medicines used as hepatoprotective, although different research works are going on some drug. Herbal drugs are more widely used than allopathic drugs as hepatoprotectives because they are inexpensive, have better cultural acceptability, better compatibility with the human body and minimal side effects. The present review discusses different types of recently herbal plants containing hepatoprotective activity. INTRODUCTION:Liver is a vital organ of paramount importance involved in the maintenance of metabolic functions and detoxification of the exogenous and endogenous challenges like xenobiotics, drugs, viral infections and chronic alcoholism 1 .Diverse homeostatic mechanisms are affected if liver function is impaired, with potentially serious consequences. About 20, 000 deaths occur every year due to liver diseases. Hepatocellular carcinoma is one of the ten most common tumors in the world with over 2, 50,000 new cases each year.

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“…Melastoma malabathricum possesses a myriad of chemicals exhibiting antibacterial (Alwash, 2013;Choudhury et al, 2011;Omar et al, 2012), anti-inflammatory, antioxidant (Susanti et al, 2008), antidiarrheal (Sunilson et al, 2009, antiulcer (Balamurugan et al, 2013), antidiabetic (Balamurugan et al, 2014), and hepatoprotective properties (Kamisan et al, 2013). Biological activities of M. malabathricum are closely mediated via a wide range of bioactive chemical constituents such as flavonoids, flavonoid glycosides, terpenoids, steroids, anthocyanins, and tannins (Joffry et al, 2012;Susanti et al, 2008;Wong et al, 2012;Yoshida et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Metabolic fingerprinting of Melastoma malabathricum L. extracts using high-performance liquid chromatography-diode array detector combined with chemometric data analysis

Mayasari¹,

Bayu²,

Tunjung³

et al. 2021

J Appl Pharm Sci

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Melastoma malabathricum L. is an essential herb that has been traditionally used to treat several ailments such as diarrhea, dysentery, wound, and stomachache. This plant is common across tropical Indo-Pacific archipelagos distributed in various geographical locations of Indonesia. In this study, an effective, accurate, and efficient highperformance liquid chromatography (HPLC) coupled to diode array detectors was developed to evaluate the effect of geographical variations on secondary metabolites of M. malabathricum samples through establishing a fingerprint profile, identification of variations of chromatogram peaks, and determination of anti-methicillin-resistant Staphylococcus aureus (MRSA) activity. Fingerprinting analysis of the extracts was analyzed using chemometric tools including unsupervised data analysis: principal component analysis (PCA) and hierarchical cluster analysis (HCA). PCA values were identified as principal component 1 (PC1) and principal component 2 with 41.229% and 19.208%, respectively. Anti-MRSA activity of all samples showed significant inhibition against MRSA bacteria. The results indicate the significance of considering geographic distribution during field-collection efforts since they demonstrate regional metabolic variations in secondary metabolites of M. malabathricum L., as illustrated by HPLC and their anti-MRSA activity. The results also confirm the utility of this approach as a comprehensive evaluation of metabolic variations based on their different geographical locations and pharmacological effects.

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Hepatoprotective Activity of Methanol Extract of Melastoma malabathricum Leaf in Rats

Cited by 24 publications

References 11 publications

Leonotis nepetifolia Protects against Acetaminophen-Induced Hepatotoxicity: Histological Studies and the Role of Antioxidant Enzymes

Leonotis nepetifolia Protects against Acetaminophen-Induced Hepatotoxicity: Histological Studies and the Role of Antioxidant Enzymes

Untitled

Metabolic fingerprinting of Melastoma malabathricum L. extracts using high-performance liquid chromatography-diode array detector combined with chemometric data analysis

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