Hepatoprotective and Nephroprotective Activity of Artemisia absinthium L. on Diclofenac-induced Toxicity in Rats

Abstract

Background: Artemisia absinthium L. is known for its antimalarial activity however, hepatoprotective activity of aqueous extracts has also been reported but, nephroprotective activity not yet evaluated. Objective: To evaluate the hepatoprotective and nephroprotective activities of A. absinthium against diclofenac-induced toxicity on rats. Materials and Methods: Three different doses of methanol and ethyl acetate extract of A. absinthium (50, 100 and 200 mg/kg/day) were evaluated and compared with silymarin 100 mg/kg. Rats received these doses for 5 days and on the 3rd and 4th day diclofenac (50 mg/kg i.p.) was administered 1 h after treatment. Animals were sacrificed 48 h after the last injection of diclofenac. Biochemical blood parameters like aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), urea and creatinine, and histopathologic changes of liver and kidney were studied and evaluated. Results: A. absinthium reduced the elevated blood levels of ALT, AST, ALP, urea and creatinine with the methanol extract to 200 mg/kg/day being more effective. The histopathologic evaluation suggested that A. absinthium decreased hepatic and renal necrosis induced by diclofenac. Conclusions: Hepatoprotective and nephroprotective activities of methanol and ethyl acetate extract of A. absinthium were demonstrated, being methanol extract to 200 mg/kg/day the most effective. This provides scientific support for the use of medicinal plants such as A. absinthium in the treatment of liver and kidney disorders.

Tubuloside A, a phenylethanoid glycoside, alleviates diclofenac induced hepato‐nephro oxidative injury via Nrf2/HO‐1

“…29 It has been reported that treatment of ibuprofen and celecoxib, NSAID drugs, in rats at a dose of 40 mg/kg for 28 days increased liver and kidney function parameters. 30 Like these drugs, the treatment of DF in rats at a dose of 10 mg/kg intramuscularly for 14 days 31 and 50 mg/kg intraperitoneally for 2 days 32 has been reported to increase AST, ALT and ALP activity levels as well as BUN and creatinine levels. Similarly, this study found that DF increased liver enzyme activities and kidney function parameters, whereas TA treatment reduced these values.…”

Acute Toxicity and Hepatoprotective Influence of Hypericum perforatum Against Thioactamid-Induced Liver Fibrosis in Rats

“…The blood specimens collected from the jugular vein were analyzed for different biochemical parameters and the dissected livers were evaluated by histopathologic techniques. 32…”

“…The blood specimens collected from the jugular vein were analyzed for different biochemical parameters and the dissected livers were evaluated by histopathologic techniques. 32 Macroscopic Viewing of the Liver. The dissected liver organs were cleaned with normal saline (cold), and dried by the filter papers for the estimation of gross weight and gross morphological studies.…”

Effect of Artemisia vulgaris Hydro-Alcoholic Extract on Oxidative Stress and Inflammatory Damages in a Rat Model of Experimental Colitis

“…The treatment period was 7 days before induction and 3 days after. The doses of A. vulgaris extracts and the duration of treatment were selected according to previous studies (29)(30)(31). The body weight of the rats was measured at the beginning and end of the interventions using an FEW (Japan) scale with a sensitivity of 1 g.…”