Hepatoprotective, cardioprotective, and renal-protective effects of organic and conventional grapevine leaf extracts (Vitis labrusca var. Bordo) on Wistar rat tissues

Abstract

The purpose of this study was to evaluate the beneficial effects of organic and conventional grapevine (Vitis labrusca L.) leaf extracts in reducing hydrogen peroxide-induced stress in the liver, heart and kidney of Wistar rats by measuring lipids and proteins damages (carbonyl assay), as well as the activity of the antioxidant enzymes superoxide dismutase and catalase. The preincubation with 5 mg/mL of organic and conventional grapevine (Vitis labrusca L.) leaf extracts prevented both lipids and proteins oxidative damages in all tissues analyzed. The organic leaf extract was able to restore superoxide dismutase (kidney and liver) and catalase (heart) activities, which were modified by the treatment with H 2 O 2 . The conventional extract was able to restore only the catalase activity in liver and heart tissues. The beneficial effects of the V. labrusca leaf extract shown in this study could probably be important for formulating dietary supplements, as well as for developing new ingredients with improved antioxidant properties from other plant sources.

Evaluation of antioxidant activity of grapevine leaves extracts (Vitis labrusca) in liver of Wistar rats

“…grape juices (Dani et al 2007(Dani et al , 2008a and grapevine leaves extracts (Dani et al 2010). With regards to the content of catechin, the conventional extract showed higher values than the organic extract, which corroborates fi ndings in grapevine leaves extracts, both organic and conventional, of V. labrusca (Dani et al 2010, Oliboni et al 2011. Many factors can affect the amount of phenolic compounds, among them, genetic factors, climate, soil conditions, degree of ripeness, postharvest storage and growth method (organic or conventional) (Mikašauskaitė et al 2013).…”

“…This result was already observed in grapevine leaves from Vitis labrusca (Dani et al 2010), and in grape juices, where organic juice was shown to be richer in polyphenols than conventional juice (Dani et al 2008a). Grapevines leaves are rich in phenolic compounds and have shown signifi cant antioxidant activity in vitro and in vivo (Monagas et al 2006, Kosar et al 2007, Orhan et al 2007, Pari and Suresh 2008, Dani et al 2010, Oliboni et al 2011.…”

“…These results corroborate with Orhan et al (2007), they showed that ethanol extracts of grapevine leaves V. vinifera were able to reduce the damage caused by CCl 4 in the liver of rats (Orhan et al 2007). The same occurred in extracts of V. labrusca against damage caused by hydrogen peroxide (H 2 O 2 ) in liver tissue of rats in an in vitro study (Oliboni et al 2011). This benefi t of derivatives of grape was also demonstrated in a study with grape juice (V. …”

“…Recent studies showed that the extracts from grapevines leaves of the Bordo variety (V. labrusca), both organic and conventional, prevent the damage caused by hydrogen peroxide in different tissues (liver, kidney, heart and brain tissues) from Wistar rats (Dani et al 2010, Oliboni et al 2011. In these studies, the extracts reduced the levels of lipid peroxidation and protein oxidation, in addition to modulating the activity of antioxidant enzymes.…”

Use of the correlation coefficient in agricultural sciences: problems, pitfalls and how to deal with them

“…It is therefore a very popular tool for analysing data that arise in many scientific disciplines, for instance biology (e.g., Soares et al 2011, Juliá and Peris 2010, Camargo et al 2011, biomedical and medical sciences (e.g., Camacho et al 2010, Oliboni et al 2011, earth sciences (e.g., Fontana et al 2010, Rangel et al 2011, social sciences (e.g., Conte et al 2011), economics (e.g., Misztal 2011), ergonomics (e.g., Bin andRichardson 2010, Zadry et al 2011), and of course agriculture (e.g., Chełkowski et al 2000, Cheng et al 2010, Lakhesar et al 2010, Bandehagh and Hossein Zadeh Moghbeli 2011, Cherati et al 2011, Heidari Zooleh et al 2011, Herrero et al 2011, Kesavacharyulu et al 2011, Rogiers et al 2011.…”