Hepatoprotective effect of a polyherbal extract containing Andrographis Paniculata, Tinospora Cordifolia and Solanum Nigrum against paracetamol induced hepatotoxicity

Abstract

Background:Traditionally, a number of medicinal plants are used to treat various types of hepatic disorders but few of them were pharmacologically evaluated for their safety and efficacy. The combination of Andrographis paniculata (Kalmegha), Tinospora cordifolia (Guduchi), and Solanum nigrum (Kakmachi) was traditionally used in Indian System of Medicine (Ayurveda) for the treatment of various liver-related disorders.Objective:In the present study, an attempt was made to substantiate the ethnopharmacological use of a traditional formulation in hepatoprotection against paracetamol-induced hepatotoxicity.Subjects and Methods:Swiss albino mice (weight 20–25 g) were used for this study. Intraperitoneal injection of paracetamol (500 mg/kg body weight) was used to induce hepatotoxicity. Serum levels of alanine transaminase, aspartate aminotransferase, bilirubin, alkaline phosphatase, were used as indices of liver injury. In addition total cholesterol, triglyceride, low-density lipoprotein, high-density lipoprotein and creatinine were also assayed using the standard procedure.Results:Among the two different doses, pretreatment with Polyherbal extract at 500 mg/kg body weight exhibited a significant (P < 0.05) hepatoprotective activity as compared to paracetamol group.Conclusion:The polyherbal extract exhibits a significant hepatoprotective effect in vivo. The study contributes to its use in traditional Ayurveda system for the management of liver diseases.SUMMARY Traditionally, a number of medicinal plants are used to treat various types of liver disorders but few of them were pharmacologically evaluated for their safety and efficacy. Combination of Andrographis paniculata (Kalmegha), Tinospora cordifolia (Guduchi), and Solanum nigrum (Kakmachi) was traditionally used in Ayurveda for the treatment of various liver related disorders. In the present study an attempt was made to validate the ethnopharmacological use of a traditional formulation in hepatoprotection against paracetamol induced hepatotoxicity. Swiss albino mice (weight 20-25 g) were used for this study. Intraperitoneal injection (IP) of paracetamol (500 mg/kg body weight) was used to induce hepatotoxicity. Serum levels of Alanine transaminase (ALT), Aspartate Aminotransferase (AST), Bilirubin, Alkaline phosphatase (ALP),. were used as indices of liver injury. In addition total cholesterol, triglyceride, Low density lipoprotein (LDL), High density lipoprotein (HDL) and creatinine were also assayed using standard procedure. Among the two different doses, pre-treatment with Polyherbal extract at 500 mg/kg body weight exhibited a significant (P < 0.05) hepatoprotective activity as compared to paracetamol group. The polyherbal extract exhibits significant hepatoprotective effect in vivo. The study contributes to its use in traditional Ayurveda system for the management of liver diseases.

Heavy Metal Toxicity in Ayurvedic Drug Formulated using Tinospora cordifolia (Giloy) – An Approach to Probe the Veracity Behind the Blanket Assertions

“…Furthermore, T. cordifolia has been reported to effectively prevent hepatotoxicity induced by antitubercular drugs in rats and humans [17]. Further, the combination of Andrographis paniculata (Kalmegha), T. cordifolia (Guduchi), and Solanum nigrum (Kakmachi) was effective against paracetamol-induced hepatotoxicity [18]. All these studies support the hepatoprotective property of T. cordifolia.…”

Hepatoprotective activities of polyherbal formulations: A systematic review

“…Figure 1 and Figure 2 illustrate the hepatotoxins utilised in the experiments to induce liver dysfunction in animal models, as well as the frequency with which each hepatotoxin was used. They include CCl 4 (Ahmad et al 2020;Arsul, Wagh & Mayee 2011;Balkrishna, Lochab & Varshney 2022;Bera et al 2012;Gurusamy et al 2010;Nipanikar, Chitlange & Nagore 2017;Said et al 2022;Sarhadynejad et al 2016;Yogi & Mishra 2017), thioacetamide (Balkrishna et al 2022), hydrogen peroxide (H 2 O 2 ) (Ndefo et al 2021), gentamicin (Aziz et al 2017), methotrexate (MTX) (Anila et al 2015), cadmium chloride (CdCl 2 ) (Singh et al 2023), D-galactosamine (Khan et al 2015;Sachdeva, Bajpai & Razdan 2013;Shetty et al 2020), alcohol (Dey et al 2020;Kanchana & Jayapriya 2013;Nipanikar et al 2017;Rafi Reshi et al 2022;Shetty et al 2018), cisplatin , isoniazid (INH) (Sankar, Rajkumar & Sridhar 2015), acetaminophen (Ali et al 2014;Fiaz et al 2017;Gupta et al 2013;Mayuren et al 2010;Nipanikar et al 2017;Patel et al 2013;Ray, Taraphdar & Gupta 2022;Saroj, Mani & Mishra 2012;Sen et al 2015;Shakya 2011;Shrivastava & Garg 2015;…”

Recent Progress in Environmental Toxins-Induced Cardiotoxicity and Protective Potential of Natural Products

“…Miers ex Hook. f. & Thomson, belonging to the family Menispermaceae Juss., is a medicinal herb used in Ayurveda for treating varied metabolic disorders and toxic conditions ( Patgiri et al, 2014 ; Singh et al, 2015 ). Antioxidant and cardioprotective activity of Tinospora cordifolia (Willd.)…”