Hepatoprotective effect of dihydroxy piperlongumine in high cholesterol-induced non-alcoholic fatty liver disease zebrafish via antioxidant activity

Haridevamuthu, B.; Boopathi, Seenivasan; Priya, P. Snega; Sundararaman, Muthuraman; Kumar, Rajendran Saravana; Manikanadan, K; Almutairi, Bader O.; Almutairi, Mikhlid H.; Arokiyaraj, Selvaraj; Gopinath, Pushparathinam; Arockiaraj, Jesu

doi:10.1016/j.ejphar.2023.175605

Cited by 21 publications

(6 citation statements)

References 44 publications

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“…The procedures were performed with slight modifications as described above [ 8 ]. Wild-type AB strain zebrafish larvae were anaesthetized with a 0.02% tricaine solution and underwent fixation in 4% PFA at 4 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

“…By 5 days post-fertilization (dpf), all digestive organs of zebrafish larvae reach maturity. The potential for evaluating drug induced hepatotoxicity in zebrafish culminates within a week, positioning them as ideal candidates for hepatotoxic assessments and hepatoprotective screening [ 8 ]. Ethanol exposure in zebrafish larvae substantially modulates the hepatic expressions of alcohol dehydrogenase and cytochrome P450 isoforms [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of Jiuzao polysaccharides in ameliorating alcoholic fatty liver disease and modulating gut microbiota

Li,

Pei,

Chen

et al. 2024

Heliyon

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of Jiuzao polysaccharides in ameliorating alcoholic fatty liver disease and modulating gut microbiota

Li,

Pei,

Chen

et al. 2024

Heliyon

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“…Don and DZ were administered via the intraperitoneal ( i.p .) route using a 31G Ultra‐Fine™ II Insulin Syringe (6 mm, U‐100, Becton, Dickinson and Company, India) as described in our previous study (Haridevamuthu, Seenivasan, et al, 2023). In brief, each fish was anaesthetised with buffered tricaine methanesulfonate (MS222, 100 mg·L −1 ) until loss of motor coordination and then gently placed in a wet, soft sponge fish holder.…”

Section: Methodsmentioning

confidence: 99%

Daidzein ameliorates nonmotor symptoms of manganese‐induced Parkinsonism in zebrafish model: Behavioural and biochemical approach

Haridevamuthu,

Sudhakaran,

Pachaiappan

et al. 2024

British J Pharmacology

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Background and PurposeParkinson's disease (PD) is a prevalent neurodegenerative movement disorder characterized by motor dysfunction. Environmental factors, especially manganese (Mn), contribute significantly to PD. Existing therapies are focused on motor coordination, whereas nonmotor features such as neuropsychiatric symptoms are often neglected. Daidzein (DZ), a phytoestrogen, has piqued interest due to its antioxidant, anti‐inflammatory, and anxiolytic properties. Therefore, we anticipate that DZ might be an effective drug to alleviate the nonmotor symptoms of Mn‐induced Parkinsonism.Experimental ApproachNaïve zebrafish were exposed to 2 mM of Mn for 21 days and intervened with DZ. Nonmotor symptoms such as anxiety, social behaviour, and olfactory function were assessed. Acetylcholinesterase (AChE) activity and antioxidant enzyme status were measured from brain tissue through biochemical assays. Dopamine levels and histology were performed to elucidate neuroprotective mechanism of DZ.Key ResultsDZ exhibited anxiolytic effects in a novel environment and also improved intra and inter fish social behaviour. DZ improved the olfactory function and response to amino acid stimuli in Mn‐induced Parkinsonism. DZ reduced brain oxidative stress and AChE activity and prevented neuronal damage. DZ increased DA level in the brain, collectively contributing to neuroprotection.Conclusion and ImplicationsDZ demonstrated a promising effect on alleviating nonmotor symptoms such as anxiety and olfactory dysfunction, through the mitigation of cellular damage. These findings underscore the therapeutic potential of DZ in addressing nonmotor neurotoxicity induced by heavy metals, particularly in the context of Mn‐induced Parkinsonism.

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“…The lipoxidation levels were quantified by their fluorescence intensity. The fluorescence intensity was assessed using Image J software [ 24 , 25 , 26 , 27 ].…”

Section: Methodsmentioning

confidence: 99%

Ophthalmic Intervention of Naringenin Decreases Vascular Endothelial Growth Factor by Counteracting Oxidative Stress and Cellular Damage in In Vivo Zebrafish

Sudhakaran,

Chandran,

Sreekutty

et al. 2023

Molecules

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Diabetes Mellitus is a metabolic disease that leads to microvascular complications like Diabetic retinopathy (DR), a major cause of blindness worldwide. Current medications for DR are expensive and report multiple side effects; therefore, an alternative medication that alleviates the disease condition is required. An interventional approach targeting the vascular endothelial growth factor (VEGF) remains a treatment strategy for DR. Anti-VEGF medicines are being investigated as the main therapy for managing vision-threatening complications of DR, such as diabetic macular oedema. Therefore, this study investigated the effect of flavonoid naringenin (NG) from citrus fruits on inhibiting early DR in zebrafish. When exposed to 130 mM glucose, the zebrafish larvae developed a hyperglycaemic condition accompanied by oxidative stress, cellular damage, and lipid peroxidation. Similarly, when adult zebrafish were exposed to 4% Glucose, high glucose levels were observed in the ocular region and massive destruction in the retinal membrane. High glucose upregulated the expression of VEGF. In comparison, the co-exposure to NG inhibited oxidative stress and cellular damage and restored the glutathione levels in the ocular region of the zebrafish larvae. NG regressed the glucose levels and cellular damage along with an inhibition of macular degeneration in the retina of adult zebrafish and normalized the overexpression of VEGF as a promising strategy for treating DR. Therefore, intervention of NG could alleviate the domestication of alternative medicine in ophthalmic research.

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Hepatoprotective effect of dihydroxy piperlongumine in high cholesterol-induced non-alcoholic fatty liver disease zebrafish via antioxidant activity

Cited by 21 publications

References 44 publications

Efficacy of Jiuzao polysaccharides in ameliorating alcoholic fatty liver disease and modulating gut microbiota

Efficacy of Jiuzao polysaccharides in ameliorating alcoholic fatty liver disease and modulating gut microbiota

Daidzein ameliorates nonmotor symptoms of manganese‐induced Parkinsonism in zebrafish model: Behavioural and biochemical approach

Ophthalmic Intervention of Naringenin Decreases Vascular Endothelial Growth Factor by Counteracting Oxidative Stress and Cellular Damage in In Vivo Zebrafish

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