Hepatoprotective effect of polysaccharides from Boschniakia rossica on carbon tetrachloride-induced toxicity in mice

Abstract

The purpose of this study was to investigate the protective effect of polysaccharides from Boschniakia rossica against hepatotoxicity induced by carbon tetrachloride (CCl4). Boschniakia rossica polysaccharides was administered intragastrically once daily for 7 days. One hour after the final treatment, mice were treated intraperitoneally with 80 mg/kg of CCl4. CCl4-induced hepatotoxicity was manifested by increased levels of serum marker enzymes and hepatic lipid peroxidation, and by decreased potential of hepatic antioxidative defense system. CCl4 challenge also resulted in elevated serum tumor necrosis factor-α and hepatic nitric oxide level, and up-regulation of inducible nitric oxide synthase and cyclooxygenase-2 proteins of liver tissue. Pretreatment of mice with Boschniakia rossica polysaccharides reversed these altered parameters of mice with liver injury induced by CCl4. Furthermore, caspase-3 cleavage and activities, and DNA fragmentation of liver in mice treated with Boschniakia rossica polysaccharides were decreased than mice treated with CCl4 alone. Hepatoprotective effect of Boschniakia rossica polysaccharides was further demonstrated by histopathological examination of liver sections. The results indicate that Boschniakia rossica polysaccharides play a protective role in CCl4-induced acute liver injury and the hepatoprotective effect of Boschniakia rossica polysaccharides may be due to elevated antioxidative defense potentials, suppressed inflammatory responses and apoptosis of liver tissue.

BRP, a polysaccharide fraction isolated from Boschniakia rossica, protects against galactosamine and lipopolysaccharide induced hepatic failure in mice

“…The preparation of BRP was performed as previously described with a minor modification. ( 22 , 26 , 28 ) Briefly, the water-soluble crude polysaccharides were obtained from Boschniakia rossica by defatting with ethanol, hot water extraction, ethanol precipitation, deproteinization by Sevag method and repeated unfreezing process, dialysis against water and lyophilization. The yield of crude polysaccharide accounted for 3.3% of the whole plant.…”

“…( 18 – 23 ) In recent decades, polysaccharides isolated from botanical sources (mushrooms, algae, lichens and higher plants) have attracted a great deal of attention in the biomedical arena because of their relatively low toxicity and various biological properties. ( 22 – 26 ) Polysaccharides from Boschniakia rossica had been reported to have anti-oxidative and anti-inflammatory functions, and have characteristics of non-toxicity. ( 22 , 23 , 25 , 26 ) Furthermore, our previous experiment showed that oral administration of Boschniakia rossica polysaccharides play a protective role in CCl 4 -induced acute liver injury by inhibiting lipid peroxidation and suppressing inflammatory responses.…”

“…( 22 – 26 ) Polysaccharides from Boschniakia rossica had been reported to have anti-oxidative and anti-inflammatory functions, and have characteristics of non-toxicity. ( 22 , 23 , 25 , 26 ) Furthermore, our previous experiment showed that oral administration of Boschniakia rossica polysaccharides play a protective role in CCl 4 -induced acute liver injury by inhibiting lipid peroxidation and suppressing inflammatory responses. ( 26 )…”

“…( 22 , 23 , 25 , 26 ) Furthermore, our previous experiment showed that oral administration of Boschniakia rossica polysaccharides play a protective role in CCl 4 -induced acute liver injury by inhibiting lipid peroxidation and suppressing inflammatory responses. ( 26 )…”

“…The effect of BRP on acute liver failure was compared to that of silymarin, which is used clinically in Europe and Asia for the treatment of liver diseases. ( 26 )…”