Hepatoprotective effect of royal jelly, grape seed extract, and Lycium barbarum against diethylnitrosamine-induced liver toxicity in rats

Abstract

We aimed to investigate, the effects of royal jelly (RJ), grape seed extract (GSE), and Lycium barbarum extract (LBAE) against diethylnitrosamine (DEN) induced hepatotoxicity, in experimental animal model. Material and Methods: Fifty female Sprague Dawley rats were divided into five groups (n=10): Control, DEN, DEN+RJ, DEN+GSE, DEN+LBAE. DEN administrated groups were intraperitoneally (i.p.) injected with three separate administration of DEN (200 mg/kg), on the zero, fifteenth and thirtieth treatment day. Then 100 mg/kg of RJ was given to DEN+RJ group, 100 mg/kg of GSE was given to DEN+GSE group, and 400 mg/kg LBAE was given to DEN+LBAE group with the daily drinking water from day 0 for 16 weeks. Histopathologic alterations including apoptotic changes of liver were evaluated. Results: RJ, GSE, and LBAE treatments significantly reduced weight loss induced by DEN. DEN administrated rats significantly increases malondialdehyde (MDA) level. It also efficiently decreases glutathione (GSH) level and catalase (CAT), superoxide dismutase (SOD) activity. These results were significantly ameliorated by dietary supplements (p<0.05). In addition, they increased the total antioxidant status (TAS) level and decreased serum oxidative stress index (OSI), total oxidant status (TOS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transpeptidase (GGT) levels significantly (p<0.05). TUNEL positive cells were extremly pervasive in the livers of DEN group. Conclusion: Improvements were prominent in case of RJ > GSE > LBAE. Our results indicated that RJ, GSE and LBAE might be useful for prevention of hepatotoxicity induced by DEN via ameliorative effects on biochemical and oxidative stress indices.

Effect Royal Jelly against Low Protein Diet Induced Hepatic Damage in Male Rats

“…This effect is due to the following features as the polyphenol capacity, inhibitory effect on stellate cells activity, disorganization of the signal transduction pathways and expression of the cell cycle proteins. 23 Stellate cells play a crucial role in the improvement of hepatic fibrosis and oxidative stress. 2 It seems that RJ with its capability can inhibit the P38MAPK phosphorylation in activated LPS of microglia.…”

Biochemical and Histopathological Investigation of the Protective Effect of Lutein in Rat Kidney Exposed to Cisplatin

“…In addition, it has been proven in many studies that antioxidant compounds prevent oxidative stress and inflammation in Cis-induced kidney damage (35)(36)(37)(38). In this study, increased serum BUN and Cr concentrations, increased MDA levels, decreased antioxidant enzyme activities and GSH levels show that kidney damage occurs as a result of Cis administration.…”

HPLC Analysis and Protective Effect of Fractions from Ethanolic Extract of Calotropis procera (Ait.) R. Br Root Bark Against Diethylnitrosamine Induced-Hepatic Damage in Wistar Rats

“…23 The different fractions also lead to a significant drop in the level of MDA in the liver, suggesting their ability to reduce the lipid peroxidation of hepatocyte membranes, which increases with the toxic effect of DEN. 23 Phytochemical screening shows that all fractions contain triterpenes, sterols, saponosides, and reducing compounds (Table 3). The butanol fraction and residual aqueous fraction also contain flavonoids and phenolic acids (Table 3).…”