Hepatoprotective Effect of Triphala and Its Combinations - In Hepg2 Cell Lines.

Abstract

Introduction:From ancient period onwards Triphala and its combinations were used in liver disorders in Ayurveda, but its mechanism of action are not still evaluated. Triphala is the combination in equi-proportion 1:1:1, of dried fruits of Amalaki (Phyllanthus emblica Linn), Bibhitaki (Terminalia bellerica Roxb) and Haritaki (Terminalia chebula Retz). The present study evaluated the hepatoprotective effect of methanolic extract of Triphala (T) and its different combinations such as Triphala with honey [TM], Triphala with Pippali (dried fruit of Piper longum Linn) [TP] and Triphala with Yashtimadhu (rhizhomes of Glycyrrhiza glabra Linn) [TY] in HepG 2 cell lines using MTT assay against acetaminophen induced hepato toxicity. Flow cytometry was used to count the viable cells. Methodology:HepG 2 (Human hepatic carcinoma) cell line was purchased from NCCS Pune and maintained in Dulbecco's modified eagle's media. Then T, TM, TP and TY were added in to acetaminophen induced hepatotoxicity cells at five concentrations; 6.25µg/ml, 12.5 µg/ml, 25 µg/ml, 50 µg/ml and 100 µg/ml using MTT assay [(3-(4,5 dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide assay]; untreated cells and acetaminophen treated cells kept as controls. Results:The acetaminophen treated cells showed 34.15% viability in MTT assay. The T, TM, TP and TY treated groups showed increase in the percentage cell viability at 6.25µg/ml, 12.5 µg/ml, 25 µg/ml, 50 µg/ml and 100 µg/ml concentrations and the result were significant (p<0.05), when compared to acetaminophen treated group. In flow cytometry total apoptosis was 28.88% for T,39.04% for TM ,42.04% for TP and 44.12% for TY. Conclusion:The results validate that Ayurveda combination T has more hepato protective effect in HepG 2 cell line; in the increasing order TY< TP < TM < T.