Hepatoprotective Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone on CCl₄-Induced Acute Liver Injury in Mice

Abstract

In this paper, the hepatoprotective effects of 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC) on CCl(4)-induced acute liver injury in Kunming mice were investigated. DMC was administered intraperitoneally (ip) (5, 10, or 20 mg/kg of body weight) for 7 days prior to the administration of CCl(4) (0.1%, ip). Pretreatment with DMC significantly decreased activities of serum hepatic enzymes, namely alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase, γ-glutamyl transferase, and total bilirubin, and decreased the elevation of lipid peroxidation, malondialdehyde, reactive oxygen species, and protein carbonyl content. Pretreatment with DMC markedly increased activities of enzymatic antioxidants such as superoxide dismutase, catalase, glucose-6-phosphate dehydrogenase, glutathione peroxidase, glutathione S-transferase, and glutathione reductase and increased levels of nonenzymatic antioxidant markers such as reduced glutathione, total sulfhydryl groups, vitamin C, and vitamin E in liver. These results combined with liver histopathology demonstrate that DMC has potential hepatoprotective effects, which may be related to the attenuation of oxidative stress, accelerating the antioxidant cascade and inhibition of lipid peroxidation.

Evaluation of the Pharmacokinetics and Hepatoprotective Effects of Phillygenin in Mouse

“…Hepatotoxicity induced by CCl 4 is the most commonly used model system for the screening of hepatoprotective activity of plant extracts and drugs [10, 19]. Osmanthus fragrans ' petroleum ether extract showed a great hepatoprotective effect on CCl 4 -induced hepatic injury mice, and phillygenin is the main ingredient in Osmanthus fragrans ' petroleum ether extract.…”

Apoptosis induced in MCF-7 human breast cancer cells by 2′,4′-dihydroxy-6-methoxy-3,5-dimethylchalcone isolated from Eugenia aquea Burm f. leaves

“…This compound also exerts antitumor effects in vivo on a solid human tumor xenograft mouse model, using human liver cancer SMMC-7721 cells (13). Potential hepatoprotective effects, which may be associated with the attenuation of oxidative stress, accelerating the antioxidant cascade and inhibition of lipid peroxidation, have also been demonstrated by 2' ,4'-dihydroxy-6-methoxy-3,5-dimethylchalcone (14).…”

Hepatoprotective effect of phenylethanoid glycosides from Incarvillea compacta against CCl4-induced cytotoxicity in HepG2 cells

“…oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) into serum and eventually kills cells (Dai et al 2014;Yu et al 2011). Therefore, antioxidative property is claimed to be one of the mechanisms of hepatoprotective effect (Bhardwaj et al 2011).…”