Hepatoprotective effects of Curcumin-Mesoporous Silica Nanoparticles on CCl 4 -induced Hepatotoxicity Wistar rats

Abstract

It has been reported that curcumin has a hepatoprotective effect, but its low solubility limited its utilization. Recently there was so many emerging research of advanced curcumin formulation, such as nanoparticles curcumin. In our previous study, curcumin has been loaded into mesoporous silica nanoparticles (C-MSN). This study was performed to evaluate of C-MSN hepatoprotective effect in CCl4-induced rats. Sixteen rats were divided into four groups, namely normal and CCl4 control, curcumin, C-MSN group. Treatment was given according to its group for fourteen days consecutively. At day 14, three hours after the last administration, CCl4 (1.25mL/kgBB) were administered orally. Twelve hours later the rats were sacrificed, and blood samples were drawn from their hearts. Blood serum examination result revealed that C-MSN caused a significantly lower ALT and AST than CCl4 control group (851±271U/L vs 1734±275U/L; 295±155U/L vs 1348±235U/L; p<0.05). Its effect on hepatic serum level resembled curcumin group. However, the result was not supported by histology examination which showed a higher number of necrotic hepatic cells in C-MSN group than in the curcumin group (147±9 vs 80±16; p<0.05). From this study, it can be concluded that C-MSN revealed an excellent hepatoprotective property, but it was suspected that MSN itself has the toxic effect on the liver. A further study of MSN toxicity was needed to support its safety use.

Enhancement of curcumin level and hepatoprotective effect in rats through antioxidant activity following modification into nanosized particles

“…The results of scoring degeneration, leukocyte infiltration, and necrosis in this study were not in accordance with the research conducted by Hadisoewignyo et al [48], where the curcumin group had a lower score than the curcumin modified into nanosized particles group, although it was not statistically significant. In this study, it was concluded that nanocurcumin with mesoporous silica nanoparticles carriers had been widely reported to cause toxicity.…”

Curcumin-loaded mesoporous silica nanoparticles for drug delivery: synthesis, biological assays and therapeutic potential – a review

“…73 In several studies, 3-aminopropyltriethoxysilane (APTES) was employed as an amine functionalization agent on the surface of MSNs, and Cur was then loaded onto the amine functionalized-MSNs. 62,63 Hartono et al synthesized cubic MSNs (particle size and pore size around 100 and 10 nm, respectively) and mesoporous silica micronsized-particles (MSM) that were functionalized with 3-aminopropyltriethoxysilane (APTES) and then Cur was encapsulated within amine functionalized-MSN and MSM (Fig. 2).…”

“…The author of this study didn't explore the effect of MSNs in a separate group, thus it's unclear whether the increased number of necrotic hepatic cells in the Cur loaded-MSNs group was due to MSN toxicity or the high concentration of Cur. 63 Cur has been shown to have cardioprotective effects in rats with myocardial necrosis via inhibiting free radical production in rats with myocardial ischemia. 136 Yadav et al investigated the cardioprotective effects of free Cur and Cur loaded-MSNs against doxorubicin-induced myocardial toxicity in rats.…”

“…The author of this study didn't explore the effect of MSNs in a separate group, thus it's unclear whether the increased number of necrotic hepatic cells in the Cur loaded-MSNs group was due to MSN toxicity or the high concentration of Cur. 63 …”