Hepatoprotective Effects of Hovenia dulcis Extract on Acute and Chronic Liver Injuries induced by Alcohol and Carbon Tetrachloride

Abstract

Objectives : The purpose of this study was to evaluate the hepatoprotective effect of Hovenia dulcis extract on acute and chronic liver injuries induced by alcohol and CCl4 in mice and rats.Methods : In acute alcohol-induced liver injury, mice were administered Hovenia dulcis extracts (60 and 200 mg/kg) orally before and after alcohol administration. In chronic alcohol-induced liver injury, mice were administered alcohol containing liquid diet for 4 weeks. The mice were administered H. dulcis extracts (60 and 200 mg/kg) mixed with the liquid diet. In acute CCl4-induced liver injury, rats received a single dose of CCl4 (2 mL/kg in olive oil, intraperitoneally). Rats were administered H. dulcis extracts (30, 100 and 300 mg/kg) before and after CCl4 administrations. After the ends of the administrations, the serum levels of AST and ALT were measured using chemical analyzer, and γ-GTP levels were measured using spectrophotometer.Results : In acute alcohol-induced liver injury, H. dulcis extracts treated group showed significant reduction in ALT levels compared to those of control group. In chronic alcohol-induced liver injury, it inhibited weight-loss compared to normal group and showed significant reduction in AST, ALT and γ-GTP levels compared to control group. In acute CCl4-induced liver injury, it also showed significant reduction in AST, ALT levels compared to control group.Conclusions : The results show that H. dulcis extract has hepatoprotective effect in acute and chronic alcohol-induced liver injury and acute CCl4-induced liver injury. These findings suggest that H. dulcis could be a potent hepatoprotective agent.

Hovenia dulcis Thumberg: Phytochemistry, Pharmacology, Toxicology and Regulatory Framework for Its Use in the European Union

“…Several in vivo studies have been conducted to evaluate the hepatoprotective effect of H. Dulcis extract in various models of liver injuries induced by chemicals toxins [ 14 , 59 , 60 ].…”

A Randomized, Double-blind, Placebo-controlled Study to the efficacy and Safety of NMED-01 and NMED-02 in Mild Alcoholic Liver Subjects

“…NMED-01군에서 혈중 γ -GTP가 유의하게 낮아진 결과는 알코올로 인하여 손상된 간세포가 수복되었다는 것을 의미한 다. 이는 알코올로 유발한 만성 간손상 모델에서 NMED-01 을 먹은 흰쥐가 AST, ALT 증가가 억제되었다는 동물실험 결 과 46…”

“…枳椇子 에탄올추출물은 사염화탄소(CCl4) 또 는 알코올 유도 급성 간손상 동물모델에서 혈청의 AST, ALT 증가를 억제하고 [26][27][28] , MDA, hydroxyproline 증가를 억제함 이 보고되어 있다 27,29) . 枳椇子 열수추출물은 CCl4 또는 D-galactosamine/LPS 유도 간손상 모델에서 혈장 내 AST, ALT, LDH, ALP 수준을 감소시키며 6,12,24) , 알코올 유도 간손 상 동물모델에서도 혈중 알코올 농도를 저하시키고 혈장 내 AST 수준을 감소시켜 간보호 효과를 나타냄이 보고되었다 6,46) . 葛根 열수추출물은 사염화탄소 유도 급성 간손상 동물모델 에서 AST, ALP를 유의하게 감소시키고 39,47) 에탄올 유도 간 손상 동물모델에서도 ALT, AST를 유의하게 감소시켰다 48) .…”

Hepatoprotective effects of gamma-aminobutyric acid-enriched fermented Hovenia dulcis extract on ethanol-induced liver injury in mice

“…In vitro and in vivo studies have demonstrated the hepatoprotective [3,9] and anti-adipogenic [5] activities of the crude extracts of H. duclis. Kim et al [10] illustrated that H. duclis extracts significantly prevented alcohol-induced acute and chronic liver injury in mice. The extract also reduced the carbon tetrachloride (CCl 4 )-induced rise in the plasma levels of liver enzymes [10,11].…”

“…Kim et al [10] illustrated that H. duclis extracts significantly prevented alcohol-induced acute and chronic liver injury in mice. The extract also reduced the carbon tetrachloride (CCl 4 )-induced rise in the plasma levels of liver enzymes [10,11].…”