Hepatoprotective effects of lactic acid-fermented garlic extract against acetaminophen-induced acute liver injury in rats

Abstract

The aim of the present study was to investigate the protective effect of fermented garlic extract by lactic acid bacteria (LAFGE) against acetaminophen (AAP)-induced acute liver injury in rats. Here we demonstrated that rats treated with LAFGE exhibit resistance to AAP-induced liver injury accompanied by lowered plasma alanine amino transferase levels and decreased proinflammatory responses. This function of LAFGE is linked to its capacity of suppressing AAP-induced apoptosis in the liver, partly via the inhibition of MAPK phosphorylation as well as down-regulation of p53. Our findings reveal that LAFGE modulates the signaling pathways involved in hepatic apoptosis through cellular redox control, as indicated by the inhibition of lipid peroxidation, glutathione and ATP depletion, and the elevation of antioxidant enzyme activities. Taken together, these findings indicate that LAFGE ameliorates AAP-induced liver injury by preventing oxidative stress-mediated apoptosis, thereby establishing LAFGE as a potential supplement in the treatment of AAP-induced liver injury.

Bioactive Compounds and Biological Functions of Garlic (Allium sativum L.)

“…Compared with unfermented garlic extract, the fermented garlic extract by Lactobacillus plantarum BL2 (LAFGE) was able to more effectively reduce liver lipid levels and ameliorate hepatic steatosis in mice [142]. Another study revealed that LAFGE inhibited liver cell apoptosis partly by suppressing MAPK phosphorylation and down-regulating p53, which protected the liver from acetaminophen-induced injury in rats [143]. Furthermore, LAFGE was considered as a potential treatment for mild hepatic dysfunction.…”

Hepatoprotective effect of a novel lactic acid‐fermented garlic extract functional food product against acute liver injury

“…Other study showed that the hepatic steatosis was blocked in rats treated with heat-extracted aged black garlic in a high fatinduced liver steatosis rat model (Shin et al, 2014). It was also reported that LAFGE attenuated acetaminophen-induced liver injury by preventing apoptosis, inhibiting lipid peroxidation and increasing antioxidant enzymes (Lee et al, 2016). In the present study, we evaluated whether the hepatoprotective effect of LAFGE can be The hepatoprotective function of LAFGE has been reported due to its antioxidative properties, mainly attributed to the presence of organosulfur compounds like S-allyl cysteine (SAC) and cycloalliin (Allison, Lowe, & Rahman, 2006).…”

“…Other study showed that the hepatic steatosis was blocked in rats treated with heat‐extracted aged black garlic in a high fat‐induced liver steatosis rat model (Shin et al, ). It was also reported that LAFGE attenuated acetaminophen‐induced liver injury by preventing apoptosis, inhibiting lipid peroxidation and increasing antioxidant enzymes (Lee et al, ). In the present study, we evaluated whether the hepatoprotective effect of LAFGE can be enhanced by adding l ‐arginine, l ‐ornithine, and the leaf extract of licorice and artichoke, using a rat model of GalN/LPS‐induced acute liver injury.…”

“…Depending on the lactic acid bacteria, LAFGE has been reported to contain varying amount of OSCs including alliin, cycloalliin, S-ethyl cysteine (SEC), SAC, and S-methylcysteine (SMC) (Jung et al, 2013). Indeed, the hepatoprotective effect of LAFGE has been well-reported in several liver disease models (Guan et al, 2018;Lee et al, 2016;Shin et al, 2014).…”

Garlic and its Active Compounds: A Novel Strategy to Fight Diseases Through Modulating Biological Activities

“…45 Another study results indicated that garlic extract had gastroprotective effects by noteworthy healing the damage of gastric mucosa and reduction in the total microbiome. 46 Garlic extract by lactic acid bacteria ameliorates liver injury through preventing oxidative stress-mediated 47 and garlic extract has hepatoprotective effects and advocates that garlic supplementation might be a one of the good adjuvant therapy for the management of liver injury. 48 Naji and colleagues have reported that administration of clove garlic had a significant reductive effect on liver function enzymes including serum alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase levels and it also had been reported to play role in the improvement of some histological parameters.…”