Hepatoprotective Effects of Rosmarinic Acid on Ovalbumin-Induced Intestinal Food Allergy Mouse Model

Abstract

Rosmarinic acid (RA) has been proven to exert antianaphylaxis in atopic dermatitis, asthma, and allergic rhinitis. The aim of this study was to determine the hepatoprotective effects of RA on ovalbumin (OVA) challenge-induced intestinal allergy. The results exhibited that RA could relieve anaphylactic symptoms, decrease diarrhea, and prevent hypothermia in allergic mice. Moreover, the elevation of OVA specific IgE (OVA-sIgE), histamine, and mouse mast cell proteinases (mMCP-1) in the serum of OVA challenged mice were remarkably inhibited by RA. OVA challenge resulted in notable increases in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities, liver malondialdehyde (MDA) and nitic oxide (NO) levels, and a remarkable decrease in liver superoxide dismutase (SOD) activity and glutathione (GSH) level. RA treatments succeeded in improving these biochemical parameters and promote the redox homeostasis. Cytokine expression evaluation showed that RA effectively enhanced the expression of anti-inflammatory cytokines (IL-10 and FOXP-3) in the liver of OVA-challenged mice. Meanwhile, the elevation of pro-inflammatory cytokines (TNF-α, IL-4, IL-6, mMCP-1, and iNOS) were remarkably inhibited by RA. These findings suggest that RA possesses hepatoprotective effects on OVA challenge-induced liver injury. The anti-oxidative and anti-inflammatory activities of RA potentially play vital roles in this process.

Investigating the Mechanism of Microwave-Assisted Enzymolysis Synergized with Magnetic Bead Adsorption for Reducing Ovalbumin Allergenicity through Biomass Spectrometry Analysis

“…The study evaluated the IgG and IgE binding capacity of OVA and its corresponding enzymatic products through an indirect competitive ELISA. 22 A total of 100 μL of natural OVA diluted in carbonate buffer solution (CBS) was added to a 96-well microplate (Synergy H1, BioTek Instruments, Inc.) and incubated at 37 °C for 2 h. Subsequently, the plate was washed 3 times with phosphate-buffered saline with Tween 20 (PBST). A total of 250 μL of 1% fish gelatin solution was added, and the mixture was incubated at 37 °C for 2 h. The plate washing was repeated 3 times.…”

Microbiota-Derived Natural Products Targeting Cancer Stem Cells: Inside the Gut Pharma Factory

“…The health-promoting potential of plant extracts and plant-derived secondary metabolites is widely recognised [186][187][188]. Numerous beneficial effects of polyphenols on human health, such as antioxidant [189][190][191][192][193], anti-inflammatory [194][195][196], immunomodulatory [197][198][199], cardioprotective [200][201][202], neuroprotective [203][204][205], anti-carcinogenic [206][207][208], and prebiotic properties [209], have been reported. Thanks to the plethora of chemical structures they exhibit, natural anticancer compounds may act as cytotoxic agents [210][211][212], anti-mitotic agents [213], angiogenesis inhibitors [214,215], topoisomerase inhibitors [216], apoptosis inducers [217] and cancer invasion [218], migration [219] and proliferation inhibitors [220][221][222].…”