Hepatoprotective flavonoids in Opuntia ficus-indica fruits by reducing oxidative stress in primary rat hepatocytes

Abstract

Background:Liver disorder was associated with alcohol consumption caused by hepatic cellular damages. Opuntia ficus-indica fruit extracts (OFIEs), which contain betalain pigments and polyphenols including flavonoids, have been introduced as reducing hangover symptoms and liver protective activity.Objective:To evaluate hepatoprotective activity of OFIEs and isolated compounds by high-speed countercurrent chromatography (HSCCC).Materials and Methods:The extract of O. ficus-indica fruits was fractionated into methylene chloride and n-butanol. The n-butanol fraction was isolated by HSCCC separation (methylene chloride-methanol-n-butanol-water, 5:4:3:5, v/v/v/v). The hepatoprotective activity of OFIEs and isolated compounds was evaluated on rat primary hepatocytes against ethanol-induced toxicity. Antioxidative parameters such as glutathione reductase and glutathione peroxidase (GSH-Px) enzymes and the GSH content were measured.Results:Two flavonoids, quercetin 3-O-methyl ester (1) and (+)-taxifolin, and two flavonoid glycosides, isorhamnetin 3-O-β-d-glucoside (3) and narcissin (4), were isolated from the n-butanol fraction by HSCCC separation. Among them, compound 2 significantly protected rat primary hepatocytes against ethanol exposure by preserving antioxidative properties of GR and GSH-Px.Conclusions:OFIEs and (+)-taxifolin were suggested to reduce hepatic damage by alcoholic oxidative stress.SUMMARY Hepatoprotective Flavonoids were isolated from Opuntia ficus-indica by high -speed countercurrent chromatography (HSCCC).

The Effect of Taxifolin on Cisplatin-Induced Pulmonary Damage in Rats: A Biochemical and Histopathological Evaluation

“…No previous studies have investigated the effect of taxifolin on tGSH in the lung tissue or its effect on DNA oxidative damage. One study reported that GSH protected the liver tissue against ethanol-induced oxidative damage by preventing its reduction [27]. The results of the present study showed that taxifolin protected DNA from oxidative damage.…”

Opuntia ficus-indica Fruit: A Systematic Review of Its Phytochemicals and Pharmacological Activities

“…Jordanian fruit juice at 2 mL/kg bd administration reduced liver damage induced by Cyclophosphamide (CP) in mice [ 143 ]. N-butanol fractioned flavonoids at 10 and 100 µg/mL protected Sprague-Dawley rat hepatocytes against alcoholic oxidative stress, increasing cell viability up to 40% [ 144 ].…”