Hepatoprotective potentials of methanol extract of T. conophorum seeds of carbon tetrachloride induced liver damage in Wistar rats

Abstract

Background: Tetracarpidium conophorum (TC) is a tropical plant used in ethno medicine for treating various diseases including hepatic ailments. The present study investigated the effect of methanol extract of T. conophorum seeds in rats intoxicated with CCl 4 24 h and 48 h after intoxication respectively. Methods: Thirty-five male Wistar rats were distributed equally into seven groups. Group IA (control) received distilled water and olive oil (i.p), group IIA rats were intoxicated with CCl 4 in olive oil (600 mg/kg, i.p.) only on the 8th day, while groups IIIA, IVA and VA were given 100 mg/kg of sylimarin, 250 mg/kg and 500 mg/kg of methanol extract respectively for 7 days, thereafter they were intoxicated with CCl 4 on the 8th day. Groups VIA and VIIA were intoxicated with CCl 4 on the 8th day and administered 250 mg/kg and 500 mg/kg of methanol extract of T. conophorum seeds at 1 h, 6 h, 12 h, 18 h and 24 h and the animals were sacrificed 24 h after intoxication with CCl 4. This procedure was repeated for a different set of thirty-five (groups IB-VIIB) male rats but the animals were sacrificed 48 h after intoxication with CCl 4. Fasting blood sample was collected by cardiac puncture for biochemical analyses. Results: There were significant increases (p˂0.05) in serum hepatic enzyme markers (ALT, AST, ALP, and γ-GT) activities, as well as bilirubin and significant reduction in antioxidant enzymes (P˂0.05) in rats intoxicated with CCl 4 when compared to control group, but administration (pre-treatment and post-treatment) of methanol extract of T. conophorum seeds at doses of 250 and 500 mg/kg body weight and standard sylimarin drug attenuated the toxic insult of CCl 4 in a dose-dependent manner at 24 h and 48 h after intoxication respectively. Conclusions: Our findings confirm that methanol extract of TC exhibited hepatoprotective activity against CCl 4 induced liver damage.

Hepatoprotective, antioxidant and, anti-inflammatory potentials of gallic acid in carbon tetrachloride-induced hepatic damage in Wistar rats

“…In this study, a single dose of carbon tetrachloride administered to rats caused significant elevations of AST, ALT, ALP, and GGT activities when compared to the control group. Our results correlated with a previous report [ 31 ]. Conversely, treatment with GA at both the lower and higher doses attenuated the increased serum enzymes activities in CCl 4 -induced hepatic damage in Wistar rats ( Fig.…”

Sub-acute and protective effect of Cymbopogon citratus against carbon tetrachloride-induced liver damage

“…Under normal circumstances, these enzymes (AST and ALT) reside in the hepatocytes. However, these enzymes will leak into the blood stream if the liver is injured, thus raising their levels in the blood (Oriakhi et al, 2018). In a research work carried out by Eraj et al (2016) aqueous extract of C. citratus was administered at a dose of 200 mg/kg body weight orally for 15 days to healthy rabbit.…”

Phytochemical Investigation and In Vitro Antioxidant Activities of Tetracarpidium conophorum (African Walnut) Seeds

“…The seeds of T. conophorum used in this study were obtained according to method described by Oriakhi et al (13).…”