Hepcidin expression in anemia of chronic disease and concomitant iron-deficiency anemia

Cheng, Panpan; Jiao, Xia; Wang, Xuehua; Lin, Jiang‐Jen; Cai, Yingmu

doi:10.1007/s10238-010-0102-9

Cited by 63 publications

(47 citation statements)

References 33 publications

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“…The average meanSD value recorded for ACD and normal group were 11.485.78ng/mL, and 8.152.22ng/mL respectively. The results well commensurate with the findings reported by chang et al 22 In this study, the serum ferritin measured in patients of normal group was 65.6716.76ng/mL while in case of ACD groups the serum ferritin level measured was elevated to 215.5015.9ng/mL which was significantly higher than other groups. The calculated P value suggested significant difference (P≤0.0001).…”

Section: Resultssupporting

confidence: 81%

Evaluation of serum hepcidin as a biochemical marker in diagnosis of anemia of chronic disease

et al. 2018

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Background: Anemia is a serious public health problem. It affects two billion people worldwide, particularly infants and young children mainly in developing countries where its etiology is multifactorial. Anemia in infancy is generally associated with impaired cognitive and behavioral development, impaired oxygen transport and a poorer prognosis in the context of many chronic diseases or chronic infections. The Present study aims to detect the serum hepcidin and ferritin levels in chronic disease anemia and correlate the values of serum hepcidin levels with their serum ferritin levels and IL-6 levels.Methods: A total of 86 individuals were enrolled in the study. Sample for hematological evaluations were collected and estimation was carried out for biomarker estimation by ELISA method(s) using specified kit(s) procured commercially. The statistical evaluation was done using SPSS version 24.0. Analysis of variance (ANOVA) and Pearson's correlation tests were used to compare the variables and to see the correlation between the different variables.Results: In present study, we observed statistically significant lower values of RBC count, Hbgm/dl, MCV, MCH, MCHC in ACD group than the normal group. For serum hepcidin when ROC curves and Pearson’s scattered plot were made in case of ACD group; the ROC was recorded to be maximum >0.869; with a sensitivity of 84.62% and specificity 94.12% while the confidence level was 95% with an interval of 0.779 to 0.932. Further, the cutoff point determined was >72.93. Thus, the hepcidin level > 72ng/mL and above is related to the ACD. These cut off points had strong confidence interval and valuable predictive potential.Conclusions: Serum Hepcidin can be used as a simple and cost effective diagnostic marker for identification of anemia.

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Section: Resultssupporting

confidence: 81%

Evaluation of serum hepcidin as a biochemical marker in diagnosis of anemia of chronic disease

et al. 2018

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“…Peripheral blood was collected under a Mayo Clinic protocol for patients with myeloid malignancies and standard procedures were followed to isolate plasma and store aliquots at 280 C. Plasma total hepcidin levels were measured in duplicate by ELISA (USCN Life Sciences, Wuhan, China) as per the manufacturer's instructions [13,14]. Samples were initially diluted 50-to 100-fold and measurements were performed on a SpectraMax 190 plate reader (Molecular Devices, Sunnyvale, CA); the resulting data were evaluated using SoftMax Pro version 5 (Molecular Devices) software.…”

Section: Plasma Cytokine and Molecular Profilingmentioning

confidence: 99%

Associations and prognostic interactions between circulating levels of hepcidin, ferritin and inflammatory cytokines in primary myelofibrosis

et al. 2013

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Iron homeostasis is dysregulated in primary myelofibrosis (PMF), given the high prevalence of anemia, need for red blood cell (RBC) transfusions, and disease-associated inflammatory state. We measured plasma hepcidin levels in 203 consecutive PMF patients at the time of first referral; hepcidin levels were significantly higher as compared to healthy controls (P < 0.0001), and were correlated with hemoglobin of <10 g/dL, RBC transfusion requirement, serum ferritin of >500 mg/L, higher dynamic international prognostic scoring system (DIPSS)-plus risk category, the presence of circulating blasts, age of >65 years, and leukocyte count of <4 3 10 9 /L. Increased hepcidin levels predicted for inferior survival independent of six out of the eight DIPSS-plus prognostic parameters (hazard ratio [HR] 5 1.8; P 5 0.02), but not when RBC transfusion requirement, hemoglobin of <10 g/dL, or increased serum ferritin were included in the Cox model. Multivariable analysis that considered the four overlapping prognostic variables revealed that increased hepcidin (HR 5 1.9; P 5 0.03) and increased ferritin (HR 5 2.3; P 5 0.04), but not hemoglobin of <10 g/dL or RBC transfusion requirement, independently retained their significance for predicting survival. Accordingly, increased levels of both hepcidin and serum ferritin (seen in 29% of patients) predicted inferior survival independent of DIPSS-plus or increased inflammatory cytokine levels (HR 5 2.4; P 5 0.002), and could be considered in future prognostic models for PMF. Am. J.

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“…The hepcidin immunoassay utilized in this study has been shown to detect inflammation, iron deficiency and hereditary hemochromatosis. 6 Hepcidin has been proposed as a potential marker of iron bioavailability for erythropoiesis in chronic kidney disease 8 and also for identifying coexisting iron deficiency in patients with concomitant anemia of chronic disease, [39][40][41] and has been proposed as an alternative to the sTfR-F index for discriminating between iron deficiency and anemia of chronic disease. 42 A comparative study of different assays for hepcidin analysis found that although the absolute value for results at each laboratory differed significantly, results for samples correlated well and analytical variance was generally low.…”

Section: S-r Pasricha Et Almentioning

confidence: 99%

Serum hepcidin as a diagnostic test of iron deficiency in premenopausal female blood donors

Pasricha¹,

McQuilten²,

Westerman³

et al. 2011

Haematologica

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BackgroundCurrently used indicators of iron status have limitations. Hepcidin, a key regulator of iron metabolism, is reduced in iron deficiency. We sought to determine the properties of hepcidin as a diagnostic test of iron deficiency. Design and MethodsSera from female, non-anemic, whole blood donors were analyzed for hepcidin (enzymelinked immunosorbent assay), ferritin, soluble transferrin receptor and C-reactive protein. Iron deficiency was defined as (i) serum ferritin less than 15 ng/mL or (ii) soluble transferrin receptor /log(ferritin) index greater than 3.2 if the C-reactive protein concentration was less than 10 mg/L, or greater than 2.2 if the C-reactive protein concentration was greater than 10 mg/L). Receiver operating characteristic curves were plotted to determine the overall utility and identify optimal cut-points of hepcidin as a test of iron deficiency. ResultsIn 261 blood donors the prevalence of iron deficiency defined by ferritin concentration was 59/261 [22.6% (17.5, 27.7)], whereas defined by soluble transferrin receptor/log(ferritin) index it was 53/261 [20. 4% (15.4, 25.2)]. The 95% reference range of hepcidin concentration in the iron-replete population was 8.2-199.7 ng/mL. The area under the receiver operating characteristic curve for hepcidin compared with ferritin concentration less than 15 ng/mL was 0.87 (0.82, 0.92), while that compared with the soluble transferrin receptor /log(ferritin) index was 0.89 (95% CI 0.84, 0.93). For a diagnosis of iron deficiency defined by the soluble transferrin receptor/log(ferritin) index, hepcidin less than 8 ng/mL had a sensitivity of 41.5% and a specificity of 97.6%, while hepcidin less than 18 ng/mL had a sensitivity of 79.2% and a specificity of 85.6%. ConclusionsSerum hepcidin concentration may be a useful indicator of deficient iron stores. Further studies are required to evaluate the role of hepcidin in the diagnosis of iron deficiency in other groups of patients.

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Hepcidin expression in anemia of chronic disease and concomitant iron-deficiency anemia

Cited by 63 publications

References 33 publications

Evaluation of serum hepcidin as a biochemical marker in diagnosis of anemia of chronic disease

Evaluation of serum hepcidin as a biochemical marker in diagnosis of anemia of chronic disease

Associations and prognostic interactions between circulating levels of hepcidin, ferritin and inflammatory cytokines in primary myelofibrosis

Serum hepcidin as a diagnostic test of iron deficiency in premenopausal female blood donors

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