Hepcidin gene expression induced in the developmental stages of fish upon exposure to Benzo[a]pyrene (BaP)

Abstract

Hepcidin is known to be expressed in fish with bacterial challenge and iron overload. Here we first report the hepcidin expression induced in the developmental stages from embryo to fry of red sea bream (Pagarus major) and in juvenile black porgy (Acanthopagrus schlegelii B.) upon continuous waterborne exposure to BaP. The gene expression of CYP1A1 and IgL (immunoglobulin light chain) were both measured. Expression of the Pagarus major hepcidin gene (PM-hepc) was increased in post hatch fry at 24 h and 120 h exposure to BaP at concentrations of 0.1, 0.5 and 1.0 mu g/l, respectively. The gene expression pattern was comparable to that of CYP1A1 but different from that of IgL. In addition, a high number of AS-hepc2 transcripts (Acanthopagrus schlegelii B. hepcidin gene) were detected in the liver upon exposure to 1.0 mu g/l BaP. This study demonstrates that hepcidin gene expression is significantly induced in BaP-exposed red sea bream and black porgy. (C) 2008 Elsevier Ltd. All rights reserved.Fujian Science and Technology Department [20031005, 200710022]; National High Technology Research and Development Program of China (863 Program) [2007AA091406

Molecular, biochemical and histopathological studies on the ameliorative effect of vitamin C on the renal and muscle tissues of Nile tilapia fish ( Oreochromis niloticus ) affected by the usage of engine oil

“…Oil exposure strongly upregulated the expression of CYP1A1 in kidney tissue (Figure ). This result is in accordance with that reported by Wang et al (). Crude petroleum oil has been reported as a mediator in oxidative stress (Achuba et al, ).The use of antioxidants to ameliorate the deleterious effect of free radicals has been the subject of previous investigations (Morgan et al, ).…”

Measures of immune system status in young‐of‐the‐year winter flounderPseudopleuronectes americanus

“…Robertson et al (2009) found that the induction of hepcidin-2 by the bacterium (Edwardsiella ictaluri) in 1 to 2 year-old largemouth bass Micropterus dolomieu Lacépède 1802 was abolished by 17 oestradiol. Furthermore, Wang et al (2009) reported that hepcidin transcripts in juvenile black porgy Acanthopagrus schlegelii (Bleeker 1854) were significantly induced by exposure from 1⋅0 μg l −1 benzo[a]pyrene after 4 h of continuous exposure. While this study assessed P. americanus that are probably chronically exposed to contaminants, the studies that were done under acute conditions show that antimicrobial peptides can either be inhibited or induced by different compounds.…”

Benzo[a]pyrene modulation of acute immunologic responses in red Sea bream pretreated with lipopolysaccharide

“…Total RNA was extracted from the livers of fish under different treatments using the TRIzol method (Invitrogen), and then reversely transcribed into cDNA using the One‐Step TaKaRa Primescript™ RT Reagent Kit (Perfect Real‐time, TaKaRa). The 18S rRNA was used as the reference gene, and the genes specific primers (18S rRNA and hepcidin) of P. major were the same as the previous report (Wang et al, ). The amplification efficiency of the PM‐hepc and the endogenous control 18S rRNA gene were verified, which were approximately equal in qPCR assay.…”

“…Some immune‐associated genes are downregulated after exposure to 2,3,7,8‐tetrachlorodibenzo‐ p ‐dioxin (TCDD) or oil, but conversely some genes (such as interleukin‐8 and lysozyme), which are involved in proinflammatory processes, are upregulated upon oil exposure (Volz et al, ; Nakayama et al, ). Similarly, an antimicrobial peptide hepcidin gene is downregulated in largemouth bass ( Micropterus salmoides ) exposed to an endocrine disrupting compound 17‐β estradiol (Robertson et al, ), but it is upregulated in the developmental stages of red sea bream Pagrus major upon exposure to BaP (Wang et al, ). Understanding of the interaction between innate immunity and toxicant effects should improve the ecotoxicological goal of well safeguarding fish populations and even the aquatic ecosystem health (Bols et al, ).…”

“…Our previous study revealed that the short‐term exposure of BaP significantly induced hepcidin genes expression (PM‐hepc and AS‐hepc2) in the developmental stages of P. major and in juvenile Acanthopagrus schlegelii B. (Wang et al, ). To further understand the interaction between the toxic effect of BaP and the immunomodulation of fish hepcidin expression in the juvenile stage of P. major , the present work continued to investigate the prolonged exposure of BaP on the mRNA expression pattern of PM‐hepc gene after lipopolysaccharide (LPS) challenge.…”