Hepeviridae: An expanding family of vertebrate viruses

Johne, Reimar; Dremsek, Paul; Reetz, Jochen; Heckel, Gerald; Heß, Michael; Ulrich, Rainer G.

doi:10.1016/j.meegid.2014.06.024

Cited by 128 publications

(143 citation statements)

References 194 publications

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“…Genom HEV stanowi jednoniciowy (+) RNA o dłu-gości około 6,6 do 7,3 kpz (22,70). Zawiera on trzy ramki odczytu (Open Reading Frame, ORF), których sekwencje kodują szereg białek strukturalnych i niestrukturalnych powstających w procesie translacji i obróbki potranslacyjnej.…”

Section: Organizacja Genomu Wirusaunclassified

Hepatitis E virus in humans, farm animals and animals from the sylvatic environment

Bigoraj¹,

Rzeżutka²

2017

Medycyna Weterynaryjna

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Section: Organizacja Genomu Wirusaunclassified

Hepatitis E virus in humans, farm animals and animals from the sylvatic environment

Bigoraj¹,

Rzeżutka²

2017

Medycyna Weterynaryjna

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“…Tab. 1) [10][11][12][13][14][15][16][17][18][19][20][21]. Kürzlich wurde in zwei Publikationen vorgeschlagen, die bisher molekular charakterisierten HEV taxonomisch neu einzuordnen [14,15].…”

Section: Erregereigenschaftenunclassified

“…In beiden Publikationen werden vergleichbare Verwandtschaftsbeziehungen der verschiedenen Isolate durch phylogenetische Analysen belegt. Wäh-rend Smith und Koautoren [14] die Hepeviridae in zwei Genera mit vier Spezies im Genus Orthohepevirus einteilen, wird von Johne und Koautoren [15] die Einteilung in fünf Genera bevorzugt (. Tab.…”

Section: Erregereigenschaftenunclassified

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Hepatitis-E-Virus

Gesundheit¹

2015

Bundesgesundheitsbl.

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“…Currently, HEV is classified in the family Hepeviridae consisting of a single genus Hepevirus and a floating species avian hepatitis E virus (avian HEV), and the virus is a non-enveloped, positive-sense single-strand RNA virus . Within the Hepevirus genus, there exist at least four well-characterized and recognized genotypes (Johne et al, 2014;Meng et al, 2012): genotype 1 and 2 HEVs infect humans while genotype 3 and 4 HEVs are zoonotic and infect humans and several other animal species (Cossaboom et al, 2011;Meng, 2010a;Pavio et al, 2010;Yazaki et al, 2003). More recently, the Hepeviridae Study Group of the International Committee on Taxonomy of Viruses (ICTV) proposed a new classification system that divides the Hepeviridae family into two separate genera, Orthohepevirus and Piscihepevirus.…”

Section: Introductionmentioning

confidence: 99%

Construction of an infectious cDNA clone of genotype 1 avian hepatitis E virus: characterization of its pathogenicity in broiler breeders and demonstration of its utility in studying the role of the hypervariable region in virus replication

Park

Lee

Moon

et al. 2015

Journal of General Virology

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A full-length infectious cDNA clone of the genotype 1 Korean avian hepatitis E virus (avian HEV) (pT11-aHEV-K) was constructed and its infectivity and pathogenicity were investigated in leghorn male hepatoma (LMH) chicken cells and broiler breeders. We demonstrated that capped RNA transcripts from the pT11-aHEV-K clone were translation competent when transfected into LMH cells and infectious when injected intrahepatically into the livers of chickens. Gross and microscopic pathological lesions underpinned the avian HEV infection and helped characterize its pathogenicity in broiler breeder chickens. The avian HEV genome contains a hypervariable region (HVR) in ORF1. To demonstrate the utility of the avian HEV infectious clone, several mutants with various deletions in and beyond the known HVR were derived from the pT11-aHEV-K clone. The HVR-deletion mutants were replication competent in LMH cells, although the deletion mutants extending beyond the known HVR were non-viable. By using the pT11-aHEV-K infectious clone as the backbone, an avian HEV luciferase reporter replicon and HVR-deletion mutant replicons were also generated. The luciferase assay results of the reporter replicon and its mutants support the data obtained from the infectious clone and its derived mutants. To further determine the effect of HVR deletion on virus replication, the capped RNA transcripts from the wild-type pT11-aHEV-K clone and its mutants were injected intrahepatically into chickens. The HVR-deletion mutants that were translation competent in LMH cells displayed in chickens an attenuation phenotype of avian HEV infectivity, suggesting that the avian HEV HVR is important in modulating the virus infectivity and pathogenicity.

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Hepeviridae: An expanding family of vertebrate viruses

Cited by 128 publications

References 194 publications

Hepatitis E virus in humans, farm animals and animals from the sylvatic environment

Hepatitis E virus in humans, farm animals and animals from the sylvatic environment

Hepatitis-E-Virus

Construction of an infectious cDNA clone of genotype 1 avian hepatitis E virus: characterization of its pathogenicity in broiler breeders and demonstration of its utility in studying the role of the hypervariable region in virus replication

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