HepG2 spheroids as a biosensor-like cell-based system for (geno)toxicity assessment

Štampar, Martina; Žabkar, Sonja; Filipič, Metka; Žegura, Bojana

doi:10.1016/j.chemosphere.2021.132805

Cited by 14 publications

(12 citation statements)

References 46 publications

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“…Cells were grown in a three-dimensional (3D) shape called spheroids, as 3D cell models have recently been shown to better reflect in vivo conditions than traditional 2D cell models. Important advantages of culturing cells in 3D form include enhanced cell–cell and cell–matrix interactions and higher expression of liver-specific functions such as urea synthesis, albumin content, and expression of phase I and II enzymes, resulting in a more physiologically relevant model for human exposure ( Štampar et al, 2022 , Štampar et al, 2019 , Conway et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Adverse (geno)toxic effects of bisphenol A and its analogues in hepatic 3D cell model

Sendra

Štampar

Fras

et al. 2023

Environment International

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Section: Introductionmentioning

confidence: 99%

Adverse (geno)toxic effects of bisphenol A and its analogues in hepatic 3D cell model

Sendra

Štampar

Fras

et al. 2023

Environment International

Self Cite

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“…Considerable progress has been made in research on biological adverse effects with the exposure of cooking emission species through cell incubation method over the last 20 years mainly focused on the high concentrations of PhIP, MeIQx and TDA solution. Most of the past studies focused on the cell cytotoxic effects through adding the PhIP compound dissolved in DMSO 35 . Poudel firstly reported that there existed remarkable differences between the dissolved and particulate forms of PhIP in cytotoxic profiles for SHSY5Y, MRC5, and HDF cells 30 .…”

Section: Discussionmentioning

confidence: 99%

“…In the literature, PhIP and MeIQx were evaluated as potent DNA damaging agents through MTT cell viability assay and the single cell gel electrophoretic assay in HepG-2 cells extraction from home cooked and commercially available food sources 36 . Depending on the exposure concentration/time, Stampar showed that PhIP’s DMSO solution at 400 μM (89.7 μg/mL) after 72 h exposure significantly decreased cell viability in HepG-2 spheroids by approximately 24% due to the DNA damage 35 . Cooking areosol contained significant amounts of MeIQx, but with unclear association between MeIQx solution and lung cancer by culture of TA98 24 .…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo low-dose exposure of simulated cooking oil fumes to assess adverse biological effects

Wang

Guan

et al. 2022

Sci Rep

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Cooking oil fumes (COFs) represent a major indoor environmental pollutant and exhibit potent mutagenic or carcinogenic health effects caused by containing various heterocyclic aromatic amines (HAAs) and long-chain aldehydes. Despite some evaluation of the cumulative exposure of COFs to cancer cells under high concentration were evaluated, their biological adverse effects with low-dose exposure to healthy cells had been inadequately investigated. Herein, we firstly scrutinized the three selected typically toxic compounds of heterocyclic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 3,8-dimethylammidazo[4,5-f]quinoxalin-2-amine (MeIQx) and trans, trans-2,4-decadienal (TDA)) emitted from COFs. In vitro studies revealed that the PhIP, MeIQx and TDA aerosol particles were negligible toxicity to cancer cells (A549 and HepG-2) but strong cytotoxicity to normal healthy cells (HelF and L02) under 0.5–4 μg/mL low dose exposure based on the reactive oxygen species (ROS) mechanism. In vivo studies demonstrated that PhIP caused significant lung and liver damage after exposure to PhIP for 30 days with mice. These results indicated the direct proof of healthy cell damage even at low-dose exposure to HAAs and aldehydes.

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“…In the literature, PhIP and MeIQx are evalated as potent DNA damaging agents through MTT cell viability assay and the single cell gel electrophoretic assay in HepG-2 cells extraction from home cooked and commercially available food sources 34 . Depending on the exposure concentration/time, Stampar showed that PhIP's DMSO solution at 400 µM (89.7 µg/mL) after 72 h exposure signi cantly decreased cell viability in HepG-2 spheroids by approximately 24% due to the DNA damage 35 . Cooking areosol contains signi cant amounts of MeIQx, but with unclear association between MeIQx solution and lung cancer by culture of TA98 24 .…”

Section: Discussionmentioning

confidence: 99%

Low-dose biological adverse effects assessment in vitro and in vivo to heterocyclic aromatic amines and aldehydes from cooking oil fumes

Wang

Guan

et al. 2022

Preprint

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Cooking oil fumes (COFs) represent a major indoor environmental pollutant and exhibit potent mutagenic or carcinogenic health effects caused by containing various heterocyclic aromatic amines (HAAs) and long-chain aldehydes. Despite some evaluation of the relationship between cumulative exposure to COFs and lung cancer, mainly focused on high concentration exposure to cancer cells, and cell damage of HAAs and aldehydes with low dose exposure to healthy cells has been unadequately investigated. Herein, we have firstly scrutinized the toxicity of these three typical compounds of HAAs and aldehyde emission from COFs, which includes heterocyclic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 3,8-dimethylammidazo[4,5-f]quinoxalin-2-amine (MeIQx) and trans, trans-2,4-decadienal (TDA)) to both in vitro and in vivo. In vitro experiments revealed that the PhIP, MeIQx and TDA aerosol particles had negligible toxicity to cancer cells (A549 and HepG-2) but strong cytotoxicity to normal healthy cells (HelF and L02) under 0.5-4 µg/mL low dose exposure based on the reactive oxygen species (ROS) mechanism. Furthermore, the in vivo data demonstrate that PhIP causes significant damage to the lung and liver after monitoring mice after 30 days of exposure to a larger amount of PhIP. These results indicate direct proof of COFs damage even at low exposure to HAAs and aldehydes.

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HepG2 spheroids as a biosensor-like cell-based system for (geno)toxicity assessment

Cited by 14 publications

References 46 publications

Adverse (geno)toxic effects of bisphenol A and its analogues in hepatic 3D cell model

Adverse (geno)toxic effects of bisphenol A and its analogues in hepatic 3D cell model

In vitro and in vivo low-dose exposure of simulated cooking oil fumes to assess adverse biological effects

Low-dose biological adverse effects assessment in vitro and in vivo to heterocyclic aromatic amines and aldehydes from cooking oil fumes

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