Heptamethoxyflavone Reduces Phosphodiesterase Activity and T-Cell Growth in vitro

Hamada, Yui; Nakajima, Miki; Tsuzuki, Kazuna; Amakura, Yoshiaki; Yoshimura, Manabu; Okuyama, Satoshi; Furukawa, Yoshiko

doi:10.1159/000481094

Cited by 12 publications

(6 citation statements)

References 24 publications

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“…It has been shown that HMF inhibits the proliferation of splenocytes stimulated with anti-CD3/CD28 antibody. 12) The results of that study are consistent with our results that showing HMF inhibits Ag-specific T cell activation (Fig. 1).…”

Section: Discussionsupporting

confidence: 92%

Citrus Polymethoxyflavonoids, Nobiletin, Heptamethoxyflavone and Natsudaidain, Suppress T Cell Activation In Vitro and In Vivo

et al. 2020

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Regents NOB, NTD and HMF were provided by Ushio-Chemix Co. (Shizuoka, Japan). Ovalubumin (OVA) 323-339 peptide was synthesized by Sigma-Aldrich (MO, USA) and the purity was over 97%. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was obtained from Tokyo Chemical Ind. Co. Ltd. (Tokyo, Japan). Mice BALB/c mice (Japan SLC, Shizuoka, Japan) and DO11.10 mice on a BALB/c background (The Jackson Laboratory, ME, USA) were maintained under specific pathogenfree conditions with a 12-h light:dark cycle at 25 ± 2°C and 55 ± 10% relative humidity. The mice were given free access to water and food throughout the experiment. All studies were performed in accordance with the ethical guidelines for animal experimentation by the Institution of Biomedical Sciences, The University of Tokushima, Japan and were approved by the institution review board of the animal ethics committee. Proliferation Assay Splenocytes (5 x 10 5 cells/well) from DO.11.10 mice were treated with NOB, NTD or HMF for 24 h in 96-well flat-bottom plate and then further cultured with 5 µg/mL OVA 323-339 peptide for 48 h at 37°C in a total volume 100 μL. Twenty μL of MTT solution was added to the culture 4 h before the end of culture. Fifty μL of 10% SDS solution was added to the well and incubated overnight at 37°C. Absorbance at 550/630 nm was measured using a microplate reader. Cytokine Production Splenocytes (2.5×10 6 cells/well) from DO11.10 mice were pretreated with NOB, NTD or HMF in a 48-well flat-bottom plate at 37°C under 5% CO 2 for 24 h and then the cells were stimulated with 5 µg/mL OVA OVA 323-339 peptide for 48 h. After the culture, culture superna

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Section: Discussionsupporting

confidence: 92%

Citrus Polymethoxyflavonoids, Nobiletin, Heptamethoxyflavone and Natsudaidain, Suppress T Cell Activation In Vitro and In Vivo

et al. 2020

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“…Aibinu et al (2007) reported that the diluted form of the C. aurantifolia fruit juice is used for mouth wash to treat sore mouth and sore throat. The fruits of C. aurantium are sources of flavonoid-type compounds with diverse biological effects (Kang et al, 2011;Hamada et al, 2017;Liu et al, 2008) besides the essential oil and its components (Moraes et al, 2009;Barceloux, 2008). Additionally, Zhang et al (2017) reported the isolation of flavonoid glycosides from the plant and the determination of biogenic amine and flavanone contents by Pellati et al (2004) and Bagatela et al (2015).…”

Section: Introductionmentioning

confidence: 99%

Effect of Citrus aurantifolia juice on bodyweight and haematological indices of wistar rats

Ezeigwe¹,

Okpala²,

Enemali³

et al. 2022

Afr. J. Food Sci.

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Citrus aurantifolia (Lime) is a citrus fruit and an excellent source of vitamin C and flavonoids which have unique antioxidant properties. This study determined the effects of C. aurantifolia on the bodyweight and hematological indices of rats. A total of 20 rats of both sex weighing between 120 and 130 g were randomized into 4 groups of five rats each and used. Group A: Normal Control; Group B: 2 ml/kg of C. aurantifolia fruit juice; Group C: 4 ml/kg of C. aurantifolia fruit juice; Group D: 8 ml/kg of C. aurantifolia fruit juice. The C. aurantifolia fruit was freshly squeezed and the juice was administered to the test groups for a period of three months. The haematological parameters were analysed using standard methods. The results revealed that administration of C. aurantifolia juice caused a significant difference (p<0.05) in the bodyweights of the experimented groups from week 3 up to week 12 compared with the normal control group. After three months of the administration, only the platelet counts showed a significant difference (p>0.05) in group B compared with other groups throughout the three months of administration. Therefore, it is then concluded that bioactive substances present in lime like lycopene and vitamin C induces the proliferation of white blood cells in the blood circulation. Hence, it was proven that lime has a protective effect which may serve as an alternative treatment option in patients with leukopenia.

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“…C. aurantium acts as an appetite suppressant and was prioritized for the replacement of ephedra-containing ( Ephedra sinica L.) weight loss products [ 4 , 5 , 6 ]. Fruits of C. aurantium are a source of flavonoid-type compounds with diverse biological effects [ 7 , 8 , 9 ]. Additionally, the presence of flavonoid glycosides [ 10 ], biogenic amines, and flavanones has been reported [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model

et al. 2020

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The main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of Citrus aurantium essential oil (CAEO). The biofilm profile of Stenotrophonomonas maltophilia and Bacillus subtilis were assessed using the mass spectrometry MALDI-TOF MS Biotyper and the antibiofilm activity of Citrus aurantium (CAEO) was studied on wood and glass surfaces. A semi-quantitative composition using a modified version was applied for the CAEO characterization. The antioxidant activity of CAEO was determined using the DPPH method. The antimicrobial activity was analyzed by disc diffusion for two biofilm producing bacteria, while the vapor phase was used for three penicillia. The antibiofilm activity was observed with the agar microdilution method. The molecular differences of biofilm formation on different days were analyzed, and the genetic similarity was studied with dendrograms constructed from MSP spectra to illustrate the grouping profiles of S. maltophilia and B. subtilis. A differentiated branch was obtained for early growth variants of S. maltophilia for planktonic cells and all experimental groups. The time span can be reported for the grouping pattern of B. subtilis preferentially when comparing to the media matrix, but without clear differences among variants. Furthermore, the minimum inhibitory doses of the CAEO were investigated against microscopic fungi. The results showed that CAEO was most active against Penicillium crustosum, in the vapor phase, on bread and carrot in situ.

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Heptamethoxyflavone Reduces Phosphodiesterase Activity and T-Cell Growth in vitro

Cited by 12 publications

References 24 publications

Citrus Polymethoxyflavonoids, Nobiletin, Heptamethoxyflavone and Natsudaidain, Suppress T Cell Activation <i>In Vitro</i> and <i>In Vivo</i>

Citrus Polymethoxyflavonoids, Nobiletin, Heptamethoxyflavone and Natsudaidain, Suppress T Cell Activation <i>In Vitro</i> and <i>In Vivo</i>

Effect of Citrus aurantifolia juice on bodyweight and haematological indices of wistar rats

Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model

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