HER1-based vaccine: Simultaneous activation of humoral and cellular immune response

Báez, Gretchen Bergado; Fernández, Diana; Herrera, Zaima Mazorra; Ramírez, Belinda Sánchez

doi:10.1053/j.seminoncol.2018.05.002

Cited by 6 publications

(2 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results with AddaVax suggest that the promotion of CD4 + T cell positioning to Th1-inducing niches may be a shared property among effective oil adjuvants, though a more comprehensive study directly comparing available oil adjuvants is needed. Nevertheless, Improvements to the formulation of oil emulsions have led to a renewed Interest In their clinical application and reopened their potential as a key component of protective vaccines against viruses and cancer ( Bergado Baez et al, 2018 ; Del Giudice et al, 2018 ). Here, we identify an Important mechanism by which oil emulsion can enhance the immune response that highlights how microenvironments within the LN can shape the immune response.…”

Section: Discussionmentioning

confidence: 99%

Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization

et al. 2020

View full text Add to dashboard Cite

SUMMARY Generating robust CD4 + T-helper cell type 1 (Th1) responses is essential for protective vaccine-induced type 1 immunity. Here, we examine whether immunization formulation associated with enhanced vaccine efficacy promotes antigen targeting and cell recruitment into lymph node (LN) niches associated with optimal type 1 responses. Immunization with antigen and Toll-like receptor agonist emulsified in oil leads to an increased differentiation of IFNγ/TNF-α + polyfunctional Th1 cells compared to an identical immunization in saline. Oil immunization results in a rapid delivery and persistence of antigen in interfollicular regions (IFRs) of the LN, whereas without oil, antigen is distributed in the medullary region. Following oil immunization, CXCL10-producing inflammatory monocytes accumulate in the IFR, which mobilizes antigen-specific CD4 + T cells into this niche. In this microenvironment, CD4 + T cells are advantageously positioned to encounter arriving IL-12-producing inflammatory dendritic cells (DCs). These data suggest that formulations delivering antigen to the LN IFR create an inflammatory niche that can improve vaccine efficacy.

show abstract

Section: Discussionmentioning

confidence: 99%

Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization

et al. 2020

View full text Add to dashboard Cite

show abstract

“…On the other hand, the protein ECD-Her1 is one of the four extracellular domains of the epidermal growth factor receptor (EGFR) pivotal in anti-cancer vaccine development León et al (2009) ; Duardo et al (2015) ; Caballero et al (2017) ; Enrico Bena (2019) . Its potential as a therapeutic vaccine has been demonstrated in preclinical studies conducted in mice with lung cancer, highlighting its antimetastatic properties and pro-apoptotic response ( Ramírez et al, 2008 ; Ramírez et al, 2006 ; Báez et al, 2018 ). Moreover, it has shown safety and immunogenicity in patients with hormonal castration-resistant prostate carcinoma ( Caballero et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Sodium acetate increases the productivity of HEK293 cells expressing the ECD-Her1 protein in batch cultures: experimental results and metabolic flux analysis

Pérez-Fernández,

Calzadilla,

Enrico Bena

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Human Embryonic Kidney cells (HEK293) are a popular host for recombinant protein expression and production in the biotechnological industry. This has driven within both, the scientific and the engineering communities, the search for strategies to increase their protein productivity. The present work is inserted into this search exploring the impact of adding sodium acetate (NaAc) into a batch culture of HEK293 cells. We monitored, as a function of time, the cell density, many external metabolites, and the supernatant concentration of the heterologous extra-cellular domain ECD-Her1 protein, a protein used to produce a candidate prostate cancer vaccine. We observed that by adding different concentrations of NaAc (0, 4, 6 and 8 mM), the production of ECD-Her1 protein increases consistently with increasing concentration, whereas the carrying capacity of the medium decreases. To understand these results we exploited a combination of experimental and computational techniques. Metabolic Flux Analysis (MFA) was used to infer intracellular metabolic fluxes from the concentration of external metabolites. Moreover, we measured independently the extracellular acidification rate and oxygen consumption rate of the cells. Both approaches support the idea that the addition of NaAc to the culture has a significant impact on the metabolism of the HEK293 cells and that, if properly tuned, enhances the productivity of the heterologous ECD-Her1 protein.

show abstract

Immunotherapy and immunoengineering for breast cancer; a comprehensive insight into CAR-T cell therapy advancements, challenges and prospects

Bozorgi

Khazaei

2022

Cell Oncol.

View full text Add to dashboard Cite

HER1-based vaccine: Simultaneous activation of humoral and cellular immune response

Cited by 6 publications

References 95 publications

Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization

Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization

Sodium acetate increases the productivity of HEK293 cells expressing the ECD-Her1 protein in batch cultures: experimental results and metabolic flux analysis

Immunotherapy and immunoengineering for breast cancer; a comprehensive insight into CAR-T cell therapy advancements, challenges and prospects

Contact Info

Product

Resources

About