HER2 overexpression and correlation with other significant clinicopathologic parameters in Ivorian breast cancer women

Aman, Nguiessan Alphonse; Doukouré, Brahima; Koffi, K; Koui, Baumaney Sylvanus; Traore, Zié Cheick; Kouyaté, M.; Effi, Ahoua Benjamin

doi:10.1186/s12907-018-0081-4

Cited by 27 publications

(15 citation statements)

References 44 publications

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“…The quantitated HER2 levels in FFPE samples allows us to investigate its correlation with other clinicopathologic factors including age, histological grade by Nottingham grading system, tumor size and metastasis status. HER2 levels were found to be associated significantly with histological grade based on Nottingham grading system using Spearman’s rank correlation analysis (ρ = 0.195, p = 0.001), a conclusion consistent with previous studies based on IHC analysis 19 , 20 . In the same study, we found that age was negatively associated with HER2 with statistical significance based on IHC analysis (ρ = − 0.117, p < 0.05), but not based on the absolutely quantitated HER2 levels (ρ = − 0.084, p = 0.127) (Supplementary Table 2 ).…”

Section: Resultssupporting

confidence: 89%

Developing a routine lab test for absolute quantification of HER2 in FFPE breast cancer tissues using Quantitative Dot Blot (QDB) method

Zhang²,

Zhang³

et al. 2020

Sci Rep

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Developing immunoassay for absolute quantitation of protein biomarkers in Formalin Fixed Paraffin Embedded (FFPE) samples promises improved objectivity, consistency and accuracy in daily clinical practice. The feasibility of Quantitative Dot Blot (QDB) method for this purpose was explored in this study. We were able to measure HER2 protein levels using 0.5 µg/sample total protein lysate extracted from 2 × 5 µm FFPE slices absolutely and quantitatively using QDB method in 332 breast cancer FFPE samples. HER2 levels measured using two clinically validated antibodies for immunohistochemistry respectively were highly correlated (r = 0.963). We also achieved area under the curve (AUC) at 0.9998 ± 0.0002 (p < 0.0001, n = 224) with IHC analysis, and 0.9942 ± 0.0031 (p < 0.0001, n = 319) with combined results from IHC and Fluorescence in situ hybridization (FISH) analyses when analyzed with Receiver Operative Characteristics analysis (ROC) respectively. When the results were converted dichotomously with optimized cutoffs from ROC analyses, we achieved 99.5% concordance with IHC; and 96.9% with combined results from both IHC and FISH analyses. Therefore, we were able to demonstrate QDB method as the first immunoassay platform for absolute quantitation of protein biomarkers in FFPE samples to meet the need of daily clinical practice, especially for local laboratories or laboratories in developing countries.

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Section: Resultssupporting

confidence: 89%

Developing a routine lab test for absolute quantification of HER2 in FFPE breast cancer tissues using Quantitative Dot Blot (QDB) method

Zhang²,

Zhang³

et al. 2020

Sci Rep

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“…HER2 positive is related to a greater mitotic index, which is one of the components of the Nottingham grade that indicates the severity of BC (29,30) . The overexpression of HER2, the high tumor grade, and the young age of our study population were unfavourable clinicopathologic factors, which are in accordance with the literature data of some studies (31,32) . In contrast, these patients should receive anti-HER2 targeted therapy and chemotherapy, which likely improve their survival.…”

Section: Discussionsupporting

confidence: 90%

Frequency and Clinical Features of Over-Expressed HER2 in Egyptian Breast Cancer Women Patients

Mohmmed¹,

Ramadan²,

El-Saiid³

et al. 2021

The Egyptian Journal of Hospital Medicine

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Background: Overexpression of human epidermal receptor protein-2 (HER2) is correlated to a poor prognosis in breast cancer (BC) patients who respond well to anti-HER2 therapy. Objective: Therapeutic procedures for treating HER2-positive breast cancer (BC) patient address HER2 protein.According to reports, the expression of HER2 oncogene and its relationship to clinicopathological factors in BC patients is yet unknown. Patients and methods: The present study involved 50 patients who were diagnosed histologically with invasive primary breast carcinoma. The PR, ER, and HER2 immunohistochemistry testing was conducted on the formalin fixed paraffinembedded blocks of patient's breast tissue. The relationship between HER2 status and clinicopathologic diagnostic characteristics was investigated using analysis of variance and the Chi-Square Test. Results: Invasive lobular carcinoma (ILC) was the most common histological type, accounting for 86% of cases. The highest percentage of patients was grade II (66.7 %), tumour size (2-5) cm accounted for 73.3 % of cases, and lymph node metastases were present in 84 %. The majority of the individuals were diagnosed at stage II (66.7 %). The majority of patients had moderate Nottingham prognosis index (66.7 %). 60 % and 33.3 % of women tested positive for the estrogen and progesterone receptors, respectively. 53.3 % of the women tested positive for HER2. Histological type (p = 0.049*) and histopathology grade (p = 0.002**) were both significantly associated with HER2 overexpression. Conclusion: HER2 positive expression could produce further evidence about the inadequate diagnosis of BC and could be utilized for pre-choice of BC patients with HER2-overexpressing who demonstrated resistance to hormonal treatment.

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“…The overexpression of HER2 is a poor prognostic factor associated with inadequate differentiation, high cell proliferation, metastasis to lymph nodes, and often resistance to specific chemotherapy. HER2 overexpression is also associated with a higher risk of recurrence compared with that in HER2-negative mammary tumor [30]. The HER group plays an important role in the signaling pathway controlling cell growth and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Effects of ethanol extract of curry leaves (Murraya koenigii) on HER2 and caspase-3 expression in rat model mammary carcinoma

et al. 2021

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Background and Aim: Human epidermal growth factor receptor 2 (HER2/erbB2/neu) is a prognostic factor and biomarker for detecting mammary tumor malignancy. Leaves of curry (Murraya koenigii) contain alkaloid, flavonoid, and phenolic compounds that can be cytotoxic to tumor cells. Caspase-3 is an indicator of apoptosis in tumor cells. This study aimed to evaluate the effect of curry leaf extract on the expression of HER2 and caspase-3 in mammary tumor through immunohistochemical analyses. Materials and Methods: Thirty five Sprague-Dawley rats were divided into seven groups: negative control of tumor (P1), positive control of tumor (P2), tumor therapy with methotrexate (P3), and curry leaf extract doses of 300 and 400 mg/ kg body weight/BW after tumor formation (P4, P5), and before tumor formation (P6, P7). Thirty rats of six groups were injected subcutaneously into the mammary glands with 7,12-dimethylbenz(α)-anthracene DMBA) twice within 2 weeks for mammary tumor formation. At the end of the treatments, the rats were euthanized, and their mammary glands were analyzed histopathologically and immunohistochemically using HER2 and caspase-3 antibodies. Results: Regarding the expression of HER2 detected in the epithelial cell membrane of the mammary gland, P2, P3, P4, and P5 revealed positive expression, P6 and P7 showed equivocal expression, while P1 showed negative expression. Regarding caspase-3 expression in the cytoplasm of epithelial cells, it was low in P1, moderate in P2, P5, P6, and P7, and high in P3 and P4. These findings suggest that DMBA injection produced mammary tumors with HER2 as a biomarker of mammary tumor, and high caspase-3 expression in P4 was the effect of curry leaves extract. Conclusion: The extract of curry leaves at a dose of 300 mg/kg BW with preventive and curative effects can potentially be used as an anti-tumor agent, which effectively induces the apoptosis of tumor cells.

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HER2 overexpression and correlation with other significant clinicopathologic parameters in Ivorian breast cancer women

Cited by 27 publications

References 44 publications

Developing a routine lab test for absolute quantification of HER2 in FFPE breast cancer tissues using Quantitative Dot Blot (QDB) method

Developing a routine lab test for absolute quantification of HER2 in FFPE breast cancer tissues using Quantitative Dot Blot (QDB) method

Frequency and Clinical Features of Over-Expressed HER2 in Egyptian Breast Cancer Women Patients

Effects of ethanol extract of curry leaves (Murraya koenigii) on HER2 and caspase-3 expression in rat model mammary carcinoma

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