HER2 status in advanced gastric carcinoma: A retrospective multicentric analysis from Sicily

Ieni, Antonio; Barresi,; Giuffrè, Giuseppe; Caruso, Rosario Alberto; Lanzafame, Salvatore; Villari, Loredana; Salomone, Edvige; Roz, Elena; Cabibi, Daniela; Franco, Vito; Certo, Giovanna; Labate, Agata; Nagar, C; Magliolo, Emilia; Broggi, B; Fazzari, C; Italia, Fabrizio; Tuccari, Giovanni

doi:10.3892/ol.2013.1611

Cited by 29 publications

(30 citation statements)

References 23 publications

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“…The value of Ki-67 becomes more marked if it is seen in conjunction with Her2-neu positivity. An association between Her2-neu overexpression, Ki-67 proliferation index and a high grade and stage has been reported [27]. In our study 83% of Her2-neu positive cases showed a high Ki-67 proliferation index (Figure 1(b,c)).…”

Section: Discussionsupporting

confidence: 74%

Incorporation of p-53 mutation status and Ki-67 proliferating index in classifying Her2-neu positive gastric adenocarcinoma

Ahmed

Al-Tamimi

2018

Libyan Journal of Medicine

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Her2-neu overexpression has a pathogenetic, therapeutic and a controversial prognostic role in gastric cancer. p-53 mutation status and Ki-67 proliferation index are established prognostic markers in many tumors. In this study we evaluated p-53 and Ki-67 in relation to Her2-neu positive and negative gastric adenocarcinoma (GA). This cross-sectional study was carried out at King Fahd Hospital of Imam Abdulrahman bin Faisal University. Fifty cases of GA were retrieved from pathology archives. Clinico-pathological parameters were evaluated. Immunohistochemical protein analysis for Her2-neu, Ki-67 and p-53 was carried out. Fluorescent in situ hybridization (FISH) analysis was done for Her2-neu positive cases showing 2+ immunoexpression. Frequency of Ki-67 and p-53 positivity in Her2-neu positive cases was calculated and compared with those in Her2-neu negative cases. Correlation of clinicopatological parameters with Her2 positive and negative cases, p-53 mutation status and Ki-67 proliferation index was carried out. Her2-neu overexpression was present in 12% (n = 6) cases. A high Ki-67 was seen predominantly in Her2-neu positive cases (83%, n = 5). Her2-neu negative cases (n = 44) showed moderate (31.88%, n = 14) to low (34%, n = 15) Ki-67. Diffuse p-53 positivity was seen predominantly in Her2-neu positive cases (33.33%, n = 2). Focal p-53 was seen mainly in Her2-neu negative cases 56.8% (n = 25). Negative p-53 was seen to be independent of Her2-neu status. Her2-neu positivity is strongly associated with diffuse p-53 mutation status and high Ki-67 proliferation. Her 2-neu negative status is associated with focal p-53 positivity and low to moderate Ki-67 proliferation index. Such stratifications in prognostic markers could not only be predictive in patient’s prognostics but could also form a basis of molecular classification of gastric cancer.

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Section: Discussionsupporting

confidence: 74%

Incorporation of p-53 mutation status and Ki-67 proliferating index in classifying Her2-neu positive gastric adenocarcinoma

Ahmed

Al-Tamimi

2018

Libyan Journal of Medicine

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“…The HER2 gene is frequently amplified and the protein overexpressed in several human epithelial malignancies, including breast, gastric, ovarian, and colon-rectal cancers [3,4,5,6,7,8,9]. In such tumors, HER2 amplification/overexpression has been linked to a poor overall outcome and a poorly differentiated phenotype [3,4,5,6,7].…”

Section: Introductionmentioning

confidence: 99%

“…In such tumors, HER2 amplification/overexpression has been linked to a poor overall outcome and a poorly differentiated phenotype [3,4,5,6,7]. It has also been considered a useful indicator of response to specifically targeted therapies, such as trastuzumab, that inhibit the extracellular domain of HER2 [8,9].…”

Section: Introductionmentioning

confidence: 99%

HER2 Analysis in Sporadic Thyroid Cancer of Follicular Cell Origin

Ruggeri

Campennì

Giuffrè

et al. 2016

IJMS

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The Epidermal Growth Factor Receoptor (EGFR) family member human epidermal growth factor receptor 2 (HER2) is overexpressed in many human epithelial malignancies, representing a molecular target for specific anti-neoplastic drugs. Few data are available on HER2 status in differentiated thyroid cancer (DTC). The present study was aimed to investigate HER2 status in sporadic cancers of follicular cell origin to better clarify the role of this receptor in the stratification of thyroid cancer. By immunohistochemistry and fluorescence in-situ hybridization, HER2 expression was investigated in formalin-fixed paraffin-embedded surgical specimens from 90 DTC patients, 45 follicular (FTC) and 45 papillary (PTC) histotypes. No HER2 immunostaining was recorded in background thyroid tissue. By contrast, overall HER2 overexpression was found in 20/45 (44%) FTC and 8/45 (18%) PTC, with a significant difference between the two histotypes (p = 0.046). Five of the six patients who developed metastatic disease during a median nine-year follow-up had a HER2-positive tumor. Therefore, we suggest that HER2 expression may represent an additional aid to identify a subset of patients who are characterized by a worse prognosis and are potentially eligible for targeted therapy.

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“…Despite its decreasing incidence and mortality, gastric cancer (GC) still represents the second leading cause of cancer death worldwide [1–3]. Approximately, 20% of cancer deaths worldwide are associated with unresolved infection or chronic inflammation; moreover, prolonged inflammation can lead to mucosal atrophy, which in some patients precedes neoplastic development [4].…”

Section: Introductionmentioning

confidence: 99%

Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations

Ieni

Barresi

Branca

et al. 2016

Analytical Cellular Pathology

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Aim. The role of mast cells in cell-cell immune interactions has received increasing attention, although their functional interaction with neutrophils still remains to be clarified in tumors. The aim of the present study was to investigate the association between mast cells and neutrophils in a series of gastric carcinomas (GC). Patients and Methods. 52 surgically resected GC specimens were routinely processed for both light and electron microscopy. Only cases showing both mast cells and neutrophils in the tumor stroma were considered in the analysis. Results. Only 9 GC (M : F = 5 : 4; age range: 50–82 years) showed both mast cells and neutrophils in the tumor stroma. At ultrathin sections, we identified heterotypic aggregation and intermingling of mast cells and neutrophils. Mast cells had mature phenotype and showed full complement of granules with homogeneous, scroll, particle, and mixed pattern. In addition, we found normal-appearing or early apoptosis showing neutrophils. Conclusion. Our histological findings showed the likely interaction between mast cells and neutrophils in GC. We hypothesize that the granular content of mast cells may be released in small quantity through a mechanism called “kiss-and-run fusion,” which is alternative to well-known massive anaphylactic or piecemeal degranulation.

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HER2 status in advanced gastric carcinoma: A retrospective multicentric analysis from Sicily

Cited by 29 publications

References 23 publications

Incorporation of p-53 mutation status and Ki-67 proliferating index in classifying Her2-neu positive gastric adenocarcinoma

Incorporation of p-53 mutation status and Ki-67 proliferating index in classifying Her2-neu positive gastric adenocarcinoma

HER2 Analysis in Sporadic Thyroid Cancer of Follicular Cell Origin

Mast Cell Interaction with Neutrophils in Human Gastric Carcinomas: Ultrastructural Observations

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