Heracleum persicum Essential Oil Nanoemulsion: A Nanocarrier System for the Delivery of Promising Anticancer and Antioxidant Bioactive Agents

Abstract

Essential oils are important compounds for the prevention and/or treatment of various diseases in which solubility and bio-accessibility can be improved by nanoemulsion systems. Heracleum persicum oil nanoemulsion (HAE-NE) was prepared and biological properties were investigated against human breast cancer cells and normal human fibroblasts foreskin. Particle size, zeta potential and poly dispersity index were 153 nm, −47.9 mV and 0.35, respectively. (E)anethole (57.9%), terpinolene (13.8%), ɣ-terpinene (8.1%), myrcene (6.8%), hexyl butyrate (5.2%), octyl butanoate (4.5%) and octyl acetate (3.7%) was detected in nanoemulsion. Proliferation of cancer cells at IC50 = 2.32 μg/mL was significantly (p < 0.05) inhibited, and cell migration occurred at 1.5 μL/mL. The HAE-NE at 1.5, 2.5 and 3.5 µg/concentration up-regulated caspase 3 and enhanced sub-G1 peak of cell cycle with nil cytotoxic effects in the liver, kidney and jejunum of mice. Villus height, villus width, crypt depth and goblet cells in mice group fed with 10 and 20 mg/kg body weight of HAE-NE improved. Cellular redox state in the liver indicated 10 and 20 mg/kg body weight of nanoemulsion significantly up-regulated the expression of SOD, CAT and GPx genes. Heracleum persicum oil nanoemulsion could be an eco-friendly nanotherapeutic option for pharmaceutical, cosmetological and food applications.

Fabrication of nano‐encapsulated angelica (Heracleum persicum) essential oil for enriching dairy dessert: Physicochemical, rheological and sensorial properties

“…Also, PDI also reported below 0.2 for majority of produced nanoemulsions which it may be due to application of suitable sonication and high ratio of surfactant to dispersed phase (essential oil) (Table 1). The droplet size of 153 nm and polydispersity of 0.35 also reported by Bashlouei et al [5] for Heracleum persicum essential oil nanoemulsion.…”

“…At the similar attempt, Bashlouei et al [5] concluded that H. Persicum Essential Oil Nanoemulsion could considered as a promising antioxidant and anticancer natural drug in breast cancer therapy. They explained that such antioxidant properties were mainly attributed to (E)-anethole present in H. persicum essential oil.…”

“…bioactive components of this plant are volatiles, ethyl esters, n-alkenes, phenolics, flavonoids, alkaloids, terpenoids and triterpenes [2]. Also, Hexyl butyrate, anethole, octyl acetate, hexyl-2-methylbutanoate and hexyl isobutyrate were found to be the main natural phytochemicals of H. persicum essential oil [5].…”

Fabrication of a Polysaccharide-Protein/Protein Complex Stabilized Oral Nanoemulsion to Facilitate the Therapeutic Effects of 1,8-Cineole on Atherosclerosis

“…However, the low oral bioavailability and high volatility of essential oils require ODDS to achieve pharmacological actions in vivo. 8,9 Numerous nanocarriers have been developed to facilitate the oral delivery of essential oils, including various nanoemulsions, 14 Pickering emulsions, 58 inclusion compounds 59 and solid lipid nanoparticles. 60 However, limited studies indicated a comprehensive strategy for encapsulating essential oils in the interior region of nanocarriers without content loss and consolidating the outer layer of nanocarriers to prevent volatilization.…”

“…Nanoemulsions exhibit considerable potential for the encapsulation of essential oils, thereby improving the hydrophilicity and stability of essential oils. − Essential oils are loaded in the internal oil phase of nanoemulsions, which reduces their volatility and degradation in vitro and in vivo . − The emulsifier is the key factor in the preparation of nanoemulsions. Accumulating evidence proved that bovine serum albumin (BSA) is an important emulsifier for preparing nanoemulsions that possess good biocompatibility and biodegradability. , Specifically, BSA can spread on the oil–water interface via reconfiguration.…”