Background and aims Systemic Epichloë endophytes produce alkaloids that protect their grass hosts against pathogens and herbivores. These alkaloids, together with other endophyte induced changes in litter quality, may decelerate the decomposition of infected grass litter, but so far no study has tested whether the effects on decomposition rate translate into changes in N cycling in infected grasslands. Here we test if the Epichloë uncinata infection of meadow fescue, Schedonorus pratensis decelerates litter decomposition and N release, increases soil C and N accumulation and lowers the availability of mineral N in the soil under infected grass. Methods To analyze grass litter and soil attributes, samples were collected from endophyte infected (E+) and non-infected (E-) field plots, established seven years earlier. At the time of the study, the frequency of E+ plants was 80-90 % and 0-3 % in the E+ and E-plots, respectively. Litter decomposition rate and litter N release were examined using litter mesh bags, placed on field ground. Soil mineral N availability was estimated using ion exchange resin bags that were buried in the soil.Results Epichloë uncinata infection did not affect meadow fescue litter N%, litter mass loss or litter N release. Neither did soil C and N content and resin NH 4 and NO 3 contents differ between the E+ and E-grass plots. E+ litter did not decompose faster in E+ than Eplots, i.e. no home-field advantage was observed. Conclusions We did not find evidence that Epichloë uncinata infection would decelerate N cycling and reduce N mineralization in meadow fescue grasslands. This suggests that the infection may not decrease the benefit of the endophyte-grass symbiosis by reducing soil fertility.