2013
DOI: 10.1186/1472-6882-13-89
|View full text |Cite
|
Sign up to set email alerts
|

Herbal compound “Songyou Yin” attenuates hepatoma cell invasiveness and metastasis through downregulation of cytokines secreted by activated hepatic stellate cells

Abstract: BackgroundActivated hepatic stellate cells (aHSCs) play an important role in the progression of hepatocellular carcinoma (HCC). Here, we determined if cytokines secreted in response to the herbal compound “Songyou Yin” (SYY) treatment of aHSCs could influence invasiveness and metastatic capabilities of hepatoma cells.MethodsPrimary rat hepatic stellate cells (HSCs) were isolated, activated, divided into SYY treated and untreated (nSYY) groups, and conditioned media (CM-SYY and CM-nSYY, respectively) were colle… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

2
27
0

Year Published

2015
2015
2020
2020

Publication Types

Select...
8
1

Relationship

4
5

Authors

Journals

citations
Cited by 18 publications
(29 citation statements)
references
References 43 publications
2
27
0
Order By: Relevance
“…Chen [20,21] et al has shown that prostate cancer-derived CCN3 can induce M2 macrophage infiltration, relating to the construction of the prospective environment conducive to prostate cancer metastasis. Previously, we had reported that HSCs could interact with hepatoma cells via secretion of cytokines, which play a critical role in modulating the malignant phenotypic changes of HCC [22,23]. In the present study, we proved the infiltration of HSCs into the HCC milieu relating to CCN3 paracrine of HCC, and CCN3 could elevate the cytokines paracrined by HSCs relating to enhanced HCC malignancy, all of which indicate the important role of CCN3 in the crosstalk between HCC and HSCs.…”
Section: Discussionsupporting
confidence: 69%
See 1 more Smart Citation
“…Chen [20,21] et al has shown that prostate cancer-derived CCN3 can induce M2 macrophage infiltration, relating to the construction of the prospective environment conducive to prostate cancer metastasis. Previously, we had reported that HSCs could interact with hepatoma cells via secretion of cytokines, which play a critical role in modulating the malignant phenotypic changes of HCC [22,23]. In the present study, we proved the infiltration of HSCs into the HCC milieu relating to CCN3 paracrine of HCC, and CCN3 could elevate the cytokines paracrined by HSCs relating to enhanced HCC malignancy, all of which indicate the important role of CCN3 in the crosstalk between HCC and HSCs.…”
Section: Discussionsupporting
confidence: 69%
“…LX2 cells were cultured in different conditioned media, and cell migration was performed as previously described [22], (Boyden chambers, Corning, Flintshire, UK) using the Conditioned Medium (CM) of MHCC97H-Con-CM, and MHCC97H-Oxa-CM. Then, 5 × 10 4 cells in serum free DMEM were seeded into the upper chamber of each well on the membrane (8.0 μm pore size) of a 24-well plate.…”
Section: Cell Migration Ability Assaysmentioning
confidence: 99%
“…Thus, there is evidence to support the concept that aHSCs contribute to the development and progression of different types of malignancies. Similarly, aHSCs enhance hepatoma cell invasiveness and metastasis through paracrine signaling mechanisms involving cytokine secretion [ 11 , 12 ]. Furthermore, the transforming growth factor-β (TGF-β) receptor inhibitor, LY2109761, was shown to inhibit the production of TGF-β secretion by CAFs, which blocked the cross-talk between CAFs and HCC and inhibited progression of HCC [ 13 ].…”
Section: Introductionmentioning
confidence: 99%
“…Some herbal formulas have been confirmed to be effective against hepatocarcinoma metastasis. In addition to inhibiting liver cancer growth, Songyou Yin also could downregulate activated hepatic stellate cells secreted IL6, TGFβ1 and VEGF to reduce MMP2 expression and reverse epithelialmesenchymal transition, and thus inhibit invasion and metastasis in hepatocarcinoma [167] . Biejiajian pill, a classical herbal formula, could effectively inhibit HepG2 cell proliferation, adhesion and invasion [168] .…”
Section: Inhibition Of Metastasismentioning
confidence: 99%