Herbal formula Yangyinjiedu induces lung cancer cell apoptosis via activation of early growth response 1

Yang, Wenxiao; Kang, Yani; Zhao, Qiang; Bi, Ling; Jiao, Lijing; Gu, Yunzhao; Lü, Jun; Yao, Jialin; Zhou, Di; Sun, Jingyong; Zhao, Xiaodong; Xu, Ling

doi:10.1111/jcmm.14501

Cited by 18 publications

(17 citation statements)

References 39 publications

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“…Total RNA was extracted from NSCLC cells using the TRIzol reagent 27 . Briefly, 2 µg of extracted RNA were reverse transcribed into complementary DNA (cDNA) with the application of reverse transcriptase (Thermo Fisher, USA).…”

Section: Methodsmentioning

confidence: 99%

MiR-182-5p promotes the Metastasis and Epithelial-mesenchymal Transition in Non-small Cell Lung Cancer by Targeting EPAS1

Yang¹,

Yin²,

Bi³

et al. 2021

J. Cancer

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Background: Dysregulation of microRNAs (miRNAs) is associated with the pathogenesis of non-small cell lung cancer (NSCLC). However, the mechanisms through which miR-182-5p regulate NSCLC progression have not been established. This study aimed at evaluating the expression levels of miR-182-5p in human NSCLC and its function in lung cancer cells. Endothelial PAS Domain-containing protein 1 (EPAS1; also referred to as hypoxia-inducing factor 2A, HIF-2α) is a transcription factor that is responsible for induction of genes related to cell survival under hypoxia conditions. Hypoxia, an inherent feature of solid tumors, is associated with aggressive phenotypes, as well as resistance to radiotherapy and chemotherapy, which predict metastasis and poor prognosis. Methods: The Cancer Genome Atlas (TCGA) dataset was used to investigate the association between miR-182-5p expression and clinicopathological characteristics as well as prognosis of NSCLC patients. Target genes of miR-182-5p were identified using the PITA, miRmap, microT, miRanda, PicTar, and TargetScan prediction tools. Transwell assays were performed to determine the potential functions of miR-182-5p in lung cancer cells. Luciferase reporter assays were performed to analyze regulation of the putative target of miR-182-5p while western blot assays were used to validate the luciferase results. Results: miR-182-5p was found to be upregulated in NSCLC tissues and acted as an independent prognostic factor for tumor recurrence in NSCLC patients. Functionally, overexpression of miR-182-5p promoted lung cancer cell migration and invasion. Genome-wide gene expression analysis and luciferase report assays revealed that EPAS1 is a direct target of miR-182-5p. EPAS1 was negatively correlated with miR-182-5p expression in NSCLC tissues. Univariate and multivariate survival analyses identified EPAS1 as an independent prognostic factor for overall survival (OS) in NSCLC. Conclusions: These findings imply that miR-182-5p promotes NSCLC progression by targeting EPAS1 and is, therefore, a potential indicator of tumor recurrence in NSCLC patients.

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Section: Methodsmentioning

confidence: 99%

MiR-182-5p promotes the Metastasis and Epithelial-mesenchymal Transition in Non-small Cell Lung Cancer by Targeting EPAS1

Yang¹,

Yin²,

Bi³

et al. 2021

J. Cancer

Self Cite

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“…The qRT-PCR reactions were performed with Power SYBR Green PCR Master Mix (Kapa biosystems, USA), and GAPDH was used as an internal control. Then miRNAs were isolated from the total RNA using a High Pure miRNA isolation kit (TIANGEN, KR211-01, China) followed by transcribing them through RT [26]. The protein extracts were boiled in a loading buffer followed by separation on a 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis platform (SDS-PAGE).…”

Section: Clinical Datamentioning

confidence: 99%

“…The mycoplasma test of all cells was negative. miRNA and mRNA quantitative real-time PCR Total RNA of NSCLC cells was extracted using the TRIzol reagent [26]. Brie y, 2 μg of extracted RNA was reverse transcribed to cDNA with the application of reverse transcriptase (Thermo Fisher, USA).…”

Section: Clinical Datamentioning

confidence: 99%

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MiR-182-5p Promotes The Metastasis and Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer By Targeting EPAS1

Yang

Yin

et al. 2021

Preprint

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BackgroundThe dysregulation of microRNAs (miRNAs) is associated with the pathogenesis of non-small cell lung cancer (NSCLC). However, the mechanism underlying miR-182-5p regulation of NSCLC progression is largely unknown. The purpose of this study was to examine the expression levels of miR-182-5p in human NSCLC and its funvtion in lung cancer cells. MethodsThe Cancer Genome Atlas (TCGA) data set was utilized to investigate the association between miR-182-5p expression and the clinicopathological characteristics and prognosis of NSCLC patients. The target genes of miR-182-5p were identified using the prediction tools PITA, miRmap, microT, miRanda, PicTar, and TargetScan. Transwell assays was used to explore the potential function of miR-182-5p in lung cancer cells. Luciferase reporter assay were performed to analyze the regulation of putative target of miR-182-5p and western blot assays were used to validate the luciferase results.ResultsmiR-182-5p was upregulated in NSCLC tissues and acted as an independent prognostic factor of tumor recurrence in NSCLC patients. Functionally, overexpression of miR-182-5p promoted lung cancer cell migration and invasion. Genome-wide gene expression analysis and luciferase report assay revealed that Endothelial PAS Domain-containing protein 1 (EPAS1) was a direct target of miR-182-5p. EPAS1 exhibited a negative correlation with miR-182-5p expression in NSCLC tissues. Univariate and multivariate survival analysis identified EPAS1 as an independent prognostic factor for overall survival and tumor recurrence in NSCLCConclusionsThese findings demonstrate that miR-182-5p functions as a tumor promoter in NSCLC progression by targeting EPAS1. It is, therefore, a potential indicator of tumor recurrence in NSCLC patients.

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“…In the meantime, the potential of drug resistance and side effects cannot be ignored. Therefore, the needs for alternative therapeutic approaches have become more and more pressing [4] .…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology Revealed the mechanism of Platycodon grandiflorum in Lung Cancer Treatment

Chen

Zhang

Wang

et al. 2020

Preprint

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Background: Traditional Chinese Medicine has demonstrated increasingly unique advantages in the treatment of lung cancer. Through literature review, it was found out that Platycodon grandiflorum had immunomodulatory and anti-inflammatory effects, and it had a special targeting effect on the lung. Purpose：In order to determine the molecular mechanism of Platycodon grandiflorum in lung cancer treatment. Network pharmacology theory was used to do a systematic study.Methods: The active compounds of Platycodon grandiflorum were screened from TCMSP database. Then, compounds targets were predicted with the assistance of Swiss Target Prediction and STITCH. The targets of lung cancer were screened form TTD and DisGeNET database. The common targets of compounds and lung cancer were screened out for following analysis. A Protein-Protein Interaction (PPI) Network was constructed by STRING. Subsequent to topological analysis, the hub targets were screened out for KEGG pathway and GO Enrichment. Molecular docking by AutoVina was performed to investigate the binding ability between the hub targets and compounds. Results: There were 4 active compounds screened out, including Acacetin, Spinasterol, cis-Dihydroquercetin and Luteolin. There were 80 targets screened as the common target of compounds and lung cancer. After topological analysis TP53, AKT1, VEGFA, CASP3, IL6, EGFR and MAPK1 were identified as hub targets. The 7 hub targets might be involved in 81 biological annotation and in the regulation of 23 pathways to intervene lung cancer. The main functional annotation was negative regulation of apoptotic process. Almost all of the pathways were directly or indirectly associated with the PI3K-Akt signal pathway and MAPK signal pathway. According to Affinity score of molecular docking the best binding protein for Luteolin was EGFR, and the best binding protein for Acacetin was CASP3. This meant that the Platycodon grandiflorum was easier to combine these two targets than other targets.Conclusion: Our study affirmed the effectiveness of Platycodon grandiflorum in treatment of lung cancer from molecular level. And we found that EGFR and CASP3 were the most likely targets for the direct action of Platycodon grandiflorum. The most important pathways that Platycodon grandiflori might interfere with were PI3K-Akt signaling pathway and MAPK signaling pathway.

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Herbal formula Yangyinjiedu induces lung cancer cell apoptosis via activation of early growth response 1

Cited by 18 publications

References 39 publications

MiR-182-5p promotes the Metastasis and Epithelial-mesenchymal Transition in Non-small Cell Lung Cancer by Targeting EPAS1

MiR-182-5p promotes the Metastasis and Epithelial-mesenchymal Transition in Non-small Cell Lung Cancer by Targeting EPAS1

MiR-182-5p Promotes The Metastasis and Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer By Targeting EPAS1

Network Pharmacology Revealed the mechanism of Platycodon grandiflorum in Lung Cancer Treatment

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