Herbal Medicine and False-Positive Results on Lymphocyte Transformation Test

Mantani, Naoki; Sakai, Shinya; Kogure, Toshiaki; Goto, Hirozo; Shibahara, Naotoshi; Kita, Toshiaki; Shimada, Yutaka; Terasawa, Katsutoshi

doi:10.1248/yakushi.122.399

Cited by 11 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in the DLST, mitogenic activity is assessed by measuring 3 H-thymidine incorporation. Because it has been reported that some herbal medicines have in vitro mitogenic activity, false-positive results on the DLST could be a problem [8]. Thus, it should be emphasized that we did not rely solely on DLST results to make the diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Tubulointerstitial nephritis and uveitis (TINU) syndrome caused by the Chinese herb “Goreisan”

et al. 2009

View full text Add to dashboard Cite

A 58-year-old man was admitted to our hospital complaining of fever and arthralgia. His clinical course and marked ciliary hyperemia led us to suspect tubulointestinal nephritis and uveitis (TINU) syndrome, which was confirmed ophthalmologically and by renal biopsy. Results of a drug-induced lymphocyte-stimulating test were positive for the Chinese herb "Goreisan." This is the first case in which the use of "Goreisan" was causally related to TINU syndrome.

show abstract

Section: Discussionmentioning

confidence: 99%

Tubulointerstitial nephritis and uveitis (TINU) syndrome caused by the Chinese herb “Goreisan”

et al. 2009

View full text Add to dashboard Cite

show abstract

“…7 To exclude the possibility for nonspecific lymphoproliferative activity, we first conducted an inquiry survey for LTT with any commercial dietary products containing Euglena in three major medical laboratory services in Japan. 7 To exclude the possibility for nonspecific lymphoproliferative activity, we first conducted an inquiry survey for LTT with any commercial dietary products containing Euglena in three major medical laboratory services in Japan.…”

Section: Characterization Of High Ltt Positivity With the Natural Diementioning

confidence: 99%

“…To date, increasing evidence has suggested that plant materials, such as herbal medicines, may account for the false positivity on the standard LTT. 7 To exclude the possibility for nonspecific lymphoproliferative activity, we first conducted an inquiry survey for LTT with any commercial dietary products containing Euglena in three major medical laboratory services in Japan. This survey allowed us to access a total of 22 subjects who underwent LTT with suspected Euglena-containing products during the previous year.…”

Section: Characterization Of High Ltt Positivity With the Natural Diementioning

confidence: 99%

“…Besides, irrespective of pre-intake history, toki (Angelicae Radix) and ogon (Scutellariae Radix), major components of Japanese herbal medicine, yielded a high false positivity on LTT (~85.7%). 6,7 The unique T-cell activity responsible for the LTT false positivity has yet to be fully understood. Practical difficulty underlying proper standardization and validation for all the suspected drugs thus remains inconclusive and requires a better understanding of drug-and its component-specific reaction on LTT.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dietary supplement product composed of natural ingredients as a suspected cause of erythema multiforme: A case report and identification for the confident false positivity of lymphocyte transformation test

Utsunomiya

Oyama

Chino

et al. 2019

The Journal of Dermatology

View full text Add to dashboard Cite

Growing and sustainable consumption of health‐care products raises a controversial issue underlying the reliability of an in vitro diagnostic approach for adverse skin reaction. This report aimed to: (i) discuss the causative nature of a commercial dietary supplement composed of natural ingredients, particularly an Euglena‐containing product, suspicious for erythema multiforme in our exemplified case; and (ii) to address the assay suitability of the lymphocyte transformation test (LTT) for identifying allergic reaction to any ingredient(s) of the product. A Japanese elderly man developed erythema multiforme after intake of a commercially available natural dietary product, whose LTT was positive. His clinical course and positive LTT suggested a provisional diagnosis of natural dietary product‐induced eruption. We conducted an inquiry survey for the standard LTT with any commercial products containing Euglena in three major Japanese laboratory services and identified 22 subjects, almost all of whom (21/22, 95.6%) showed a positive LTT for any Euglena‐containing products as a suspected causative. Seven normal healthy volunteers who had no intake history of Euglena‐containing products showed an equivalent LTT positivity rate with the same product taken by our case; culprit components of the product included Euglena, Angelica keiskei, Barley grass and Chlorella. A cell‐free culture system and enzyme‐linked immunoassay suggest that the high LTT positivity relies on the non‐specific lymphoproliferative activity, and not contamination of uncharacterized microorganisms and endotoxins. Because of the constitutive false positivity of LTT, this assay is unreliable for in vitro supportive diagnosis of adverse skin events caused by dietary products containing particular natural ingredients, as well as herbal materials.

show abstract

“…In addition to those reports, we have encountered false-positive results in lymphocyte transformation tests (LTTs) of 17 herbs contained in Kampo formulae (9). In the LTT, mitogenic activity is quantified by measuring [ 3 H]thymidine incorporation (2).…”

mentioning

confidence: 99%

Lymphocyte Transformation Test for Medicinal Herbs Yields False-Positive Results for First-Visit Patients

Mantani

Kogure

Tamura

et al. 2003

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

Herbal Medicine and False-Positive Results on Lymphocyte Transformation Test

Cited by 11 publications

References 19 publications

Tubulointerstitial nephritis and uveitis (TINU) syndrome caused by the Chinese herb “Goreisan”

Tubulointerstitial nephritis and uveitis (TINU) syndrome caused by the Chinese herb “Goreisan”

Dietary supplement product composed of natural ingredients as a suspected cause of erythema multiforme: A case report and identification for the confident false positivity of lymphocyte transformation test

Lymphocyte Transformation Test for Medicinal Herbs Yields False-Positive Results for First-Visit Patients

Contact Info

Product

Resources

About