Herbals Used in Western Iran as Food and for Health Treatments

Younessi-Hamzekhanlu, Mehdi; Abdipour, Moslem; Dejahang, Ata; Sabzi-Nojadeh, Mohsen; Amani, Mina

doi:10.1007/978-3-030-59928-7_21

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…Plastid genetic engineering in plants has significantly progressed during the past decades and has become a promising method for the production of high-value metabolites and proteins, e.g., industrial enzymes, biopharmaceuticals, and diagnostic reagents (Clarke et al, 2013 ; De Marchis et al, 2012 ; van Eerde et al, 2019 ; Younessi-Hamzekhanlu et al, 2021 ). The diverse features of chloroplast transformation include the dynamic concept of site-specific integration of the transgene into the plastid genome (Limaye et al, 2006 ), lack of position effects and epigenetic gene silencing (Daniell et al, 2001 ; Rigano et al, 2013 ; Ruhlman et al, 2010 ), insertion of transgenes in operons (Lu et al, 2013 ), and the biosafety profile of transplastomic plants.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the recombinant human interferon-beta (rhIFN-β) gene in tobacco chloroplasts

Feizi¹,

BaghbanKohnehrouz²

2021

bta

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Chloroplast genetic engineering is a convenient method for the production of recombinant proteins by increasing the expression level of transgenes. Interferon-beta (IFN- β ) is a member of type I interferons that possess some pharmaceutical properties. The present study aimed to investigate the overexpression and production of the recombinant human IFN- β gene ( rhIFN-β ) in the tobacco chloroplast genome. For this purpose, a codon-optimized rhIFN-β was transferred to the pVSR326 plastid vector containing the aadA gene as a selectable marker. The rhIFN-β gene was then successfully introduced into the tobacco chloroplast genome by using a gene gun. The integration of the rhIFN-β gene into the chloroplast genome and the homoplasmy of the T1 progeny were confirmed by PCR and Southern blot analysis, respectively. RT-PCR and western blot analyses confirmed the transcription and translation of the rhIFN - β gene, respectively. An enzyme-linked immunosorbent assay (ELISA) showed that the rhIFN-β protein in transplastomic plants comprised approximately 2.4% of total soluble protein (TSPs). The bioassay confirmed that the rhIFN-β protein expressed in the tobacco chloroplast had a relatively high biological activity (2.9 × 10 4 IU/ml) and protected human amnionic cells against the vesicular stomatitis virus (VSV). On the basis of these findings, it can be concluded that plastid transformation can serve as an operative method for the production of pharmaceutical recombinant proteins.

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